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The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia

Simon Devos (UGent) , Laurence Van Oudenhove (UGent) , Stephan Stremersch (UGent) , Wouter Van Putte (UGent) , Riet De Rycke (UGent) , Gonzalez Van Driessche (UGent) , Jolien Vitse (UGent) , Koen Raemdonck (UGent) and Bart Devreese (UGent)
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Abstract
Outer membrane vesicles (OMVs) are small nanoscale structures that are secreted by bacteria and that can carry nucleic acids, proteins, and small metabolites. They can mediate intracellular communication and play a role in virulence. In this study, we show that treatment with the -lactam antibiotic imipenem leads to a dramatic increase in the secretion of outer membrane vesicles in the nosocomial pathogen Stenotrophomonas maltophilia. Proteomic analysis of their protein content demonstrated that the OMVs contain the chromosomal encoded Li metallo-Hactamase and L2 serine-Hactamase. Moreover, the secreted OMVs contain large amounts of two Ax21 homologs, i.e., outer membrane proteins known to be involved in virulence and biofilm formation. We show that OMV secretion and the levels of Ax21 in the OMVs are dependent on the quorum sensing diffusible signal system (DSF). More specific, we demonstrate that the S. maltophilia DSF cis-42-11 -methyl-dodecenoic acid and, to a lesser extent, the Burkholderia cenocepacia DSF cis-42-dodecenoic acid, stimulate OMV secretion. By a targeted proteomic analysis, we confirmed that DSF-induced OMVs contain large amounts of the Ax21 homologs, but not the Hactamases. This work illustrates that both quorum sensing and disturbance of the peptidoglycan biosynthesis provoke the release of OMVs and that OMV content is context dependent.
Keywords
Stenotrophomonas maltophilia, outer membrane vesicles, antibiotic resistance, proteomics, quorum sensing, PSEUDOMONAS-AERUGINOSA, PROTEOMIC ANALYSIS, VIRULENCE, PATHOGEN, PRECURSOR, DATABASE, ORYZAE

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Chicago
Devos, Simon, Laurence Van Oudenhove, Stephan Stremersch, Wouter Van Putte, Riet De Rycke, Gonzalez Van Driessche, Jolien Vitse, Koen Raemdonck, and Bart Devreese. 2015. “The Effect of Imipenem and Diffusible Signaling Factors on the Secretion of Outer Membrane Vesicles and Associated Ax21 Proteins in Stenotrophomonas Maltophilia.” Frontiers in Microbiology 6.
APA
Devos, Simon, Van Oudenhove, L., Stremersch, S., Van Putte, W., De Rycke, R., Van Driessche, G., Vitse, J., et al. (2015). The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia. FRONTIERS IN MICROBIOLOGY, 6.
Vancouver
1.
Devos S, Van Oudenhove L, Stremersch S, Van Putte W, De Rycke R, Van Driessche G, et al. The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia. FRONTIERS IN MICROBIOLOGY. 2015;6.
MLA
Devos, Simon, Laurence Van Oudenhove, Stephan Stremersch, et al. “The Effect of Imipenem and Diffusible Signaling Factors on the Secretion of Outer Membrane Vesicles and Associated Ax21 Proteins in Stenotrophomonas Maltophilia.” FRONTIERS IN MICROBIOLOGY 6 (2015): n. pag. Print.
@article{6920527,
  abstract     = {Outer membrane vesicles (OMVs) are small nanoscale structures that are secreted by bacteria and that can carry nucleic acids, proteins, and small metabolites. They can mediate intracellular communication and play a role in virulence. In this study, we show that treatment with the -lactam antibiotic imipenem leads to a dramatic increase in the secretion of outer membrane vesicles in the nosocomial pathogen Stenotrophomonas maltophilia. Proteomic analysis of their protein content demonstrated that the OMVs contain the chromosomal encoded Li metallo-Hactamase and L2 serine-Hactamase. Moreover, the secreted OMVs contain large amounts of two Ax21 homologs, i.e., outer membrane proteins known to be involved in virulence and biofilm formation. We show that OMV secretion and the levels of Ax21 in the OMVs are dependent on the quorum sensing diffusible signal system (DSF). More specific, we demonstrate that the S. maltophilia DSF cis-42-11 -methyl-dodecenoic acid and, to a lesser extent, the Burkholderia cenocepacia DSF cis-42-dodecenoic acid, stimulate OMV secretion. By a targeted proteomic analysis, we confirmed that DSF-induced OMVs contain large amounts of the Ax21 homologs, but not the Hactamases. This work illustrates that both quorum sensing and disturbance of the peptidoglycan biosynthesis provoke the release of OMVs and that OMV content is context dependent.},
  articleno    = {298},
  author       = {Devos, Simon and Van Oudenhove, Laurence and Stremersch, Stephan and Van Putte, Wouter and De Rycke, Riet and Van Driessche, Gonzalez and Vitse, Jolien and Raemdonck, Koen and Devreese, Bart},
  issn         = {1664-302X},
  journal      = {FRONTIERS IN MICROBIOLOGY},
  keyword      = {Stenotrophomonas maltophilia,outer membrane vesicles,antibiotic resistance,proteomics,quorum sensing,PSEUDOMONAS-AERUGINOSA,PROTEOMIC ANALYSIS,VIRULENCE,PATHOGEN,PRECURSOR,DATABASE,ORYZAE},
  language     = {eng},
  pages        = {9},
  title        = {The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia},
  url          = {http://dx.doi.org/10.3389/fmicb.2015.00298},
  volume       = {6},
  year         = {2015},
}

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