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Tumor-inducing (Ti) plasmids, carried by Agrobacterium tumefaciens have been shown to be responsible for crown gall formation in plants. Ti plasmids are natural gene vectors with which Agrobacteria achieve the transfer and stable maintenance of a defined DNA segment (called T­region) into the nucleus of transformed plant cells. Using site-specific mutagenesis, it was possible to introduce mutations in different parts of the T-region. The transcription of the T-DNA in wild-type and mutant crown galls was compared, and it was found that the induction of specific developmental patterns could be correlated with the absence of specific T-DNA transcripts. Double mutants were obtained in which the expres­sion of all the "onc" genes was abolished. Tobacco, potato, and petunia plant cells harboring such inactivated T-DNAs were shown to regenerate normal, fertile plants that transmit the T-DNA segment as a single Mendelian locus. Structural genes, coding for opine synthase enzymes, were shown to be fully active. Several T-DNA genes were sequenced, and transcription promoter and termination signals were identified.

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MLA
Schell, Jeff, Marc Van Montagu, Jean-Pierre Hernalsteens, et al. “Gene Vectors for Higher Plants.” Beltsville Symposia in Agricultural Research. Ed. Lowell D Owens. Vol. 7. Totowa, NJ, USA: Rowman & Allanheld, 1983. 197–213. Print.
APA
Schell, J., Van Montagu, M., Hernalsteens, J.-P., De Greve, H., Leemans, J., Koncz, C., Willmitzer, L., et al. (1983). Gene vectors for higher plants. In L. D. Owens (Ed.), Beltsville Symposia in Agricultural Research (Vol. 7, pp. 197–213). Presented at the Beltsville symposium in Agricultural Research, Totowa, NJ, USA: Rowman & Allanheld.
Chicago author-date
Schell, Jeff, Marc Van Montagu, Jean-Pierre Hernalsteens, Henri De Greve, Jan Leemans, Csaba Koncz, Lothar Willmitzer, Leon Otten, Joachim Schröder, and Gudrun Schröder. 1983. “Gene Vectors for Higher Plants.” In Beltsville Symposia in Agricultural Research, ed. Lowell D Owens, 7:197–213. Totowa, NJ, USA: Rowman & Allanheld.
Chicago author-date (all authors)
Schell, Jeff, Marc Van Montagu, Jean-Pierre Hernalsteens, Henri De Greve, Jan Leemans, Csaba Koncz, Lothar Willmitzer, Leon Otten, Joachim Schröder, and Gudrun Schröder. 1983. “Gene Vectors for Higher Plants.” In Beltsville Symposia in Agricultural Research, ed. Lowell D Owens, 7:197–213. Totowa, NJ, USA: Rowman & Allanheld.
Vancouver
1.
Schell J, Van Montagu M, Hernalsteens J-P, De Greve H, Leemans J, Koncz C, et al. Gene vectors for higher plants. In: Owens LD, editor. Beltsville Symposia in Agricultural Research. Totowa, NJ, USA: Rowman & Allanheld; 1983. p. 197–213.
IEEE
[1]
J. Schell et al., “Gene vectors for higher plants,” in Beltsville Symposia in Agricultural Research, Beltsville, MD, USA, 1983, vol. 7, pp. 197–213.
@inproceedings{6918474,
  abstract     = {Tumor-inducing (Ti) plasmids, carried by Agrobacterium tumefaciens have been shown to be responsible for crown gall formation in plants. Ti plasmids are natural gene vectors with which Agrobacteria achieve the transfer and stable maintenance of a defined DNA segment (called T­region) into the nucleus of transformed  plant cells. Using site-specific mutagenesis, it was possible to introduce mutations in different parts of the T-region. The transcription of the T-DNA in wild-type and mutant crown galls was compared, and it was found that the induction of specific developmental patterns could be correlated with the absence of specific T-DNA transcripts.  Double mutants were obtained in which the expres­sion of all the "onc" genes was abolished. Tobacco, potato, and petunia plant cells harboring such inactivated T-DNAs were shown to regenerate normal, fertile plants that transmit the T-DNA segment as a single Mendelian locus. Structural genes, coding for opine synthase enzymes, were shown to be fully active. Several T-DNA genes were sequenced, and transcription  promoter and termination signals were identified.},
  author       = {Schell, Jeff and Van Montagu, Marc and Hernalsteens, Jean-Pierre and De Greve, Henri and Leemans, Jan and Koncz, Csaba and Willmitzer, Lothar and Otten, Leon and Schröder, Joachim and Schröder, Gudrun},
  booktitle    = {Beltsville Symposia in Agricultural Research},
  editor       = {Owens, Lowell D},
  isbn         = {9780865981126},
  language     = {eng},
  location     = {Beltsville, MD, USA},
  pages        = {197--213},
  publisher    = {Rowman & Allanheld},
  title        = {Gene vectors for higher plants},
  volume       = {7},
  year         = {1983},
}