Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 5.51 MB
Add to list
  1. Search results
  2. PRC2 represses dedifferentiation of m...

PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis

Momoko Ikeuchi, Akira Iwase, Bart Rymen (UGent) , Hirofumi Harashima, Michitaro Shibata, Mariko Ohnuma, Christian Breuer, Ana Karina Morao, Miguel de Lucas, Lieven De Veylder (UGent) , et al.
(2015) NATURE PLANTS. 1(7).
Author
Organization
Abstract
Plant somatic cells are generally acknowledged to retain totipotency, the potential to develop into any cell type within an organism. This astonishing plasticity may contribute to a high regenerative capacity on severe damage, but how plants control this potential during normal post-embryonic development remains largely unknown1,2. Here we show that POLYCOMB REPRESSIVE COMPLEX 2 (PRC2), a chromatin regulator that maintains gene repression through histone modification, prevents dedifferentiation of mature somatic cells in Arabidopsis thaliana roots. Loss-of-function mutants in PRC2 subunits initially develop unicellular root hairs indistinguishable from those in wild type but fail to retain the differentiated state, ultimately resulting in the generation of an unorganized cell mass and somatic embryos from a single root hair. Strikingly, mutant root hairs complete the normal endoreduplication programme, increasing their nuclear ploidy, but subsequently reinitiate mitotic division coupled with successive DNA replication. Our data show that the WOUND INDUCED DEDIFFERENTIATION3 (WIND3) and LEAFY COTYLEDON2 (LEC2) genes are among the PRC2 targets involved in this reprogramming, as their ectopic overexpression partly phenocopies the dedifferentiation phenotype of PRC2 mutants. These findings unveil the pivotal role of PRC2-mediated gene repression in preventing unscheduled reprogramming of fully differentiated plant cells.
Keywords
MERISTEM, THALIANA, REGENERATION, ROOT, GROWTH, GENE-EXPRESSION, TRANSCRIPTION FACTOR, POLYCOMB-GROUP PROTEINS, TOPOISOMERASE-VI COMPLEX, PLANTS

Downloads

  • Download
    (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 5.51 MB

Citation

Please use this url to cite or link to this publication:

MLA
Ikeuchi, Momoko, et al. “PRC2 Represses Dedifferentiation of Mature Somatic Cells in Arabidopsis.” NATURE PLANTS, vol. 1, no. 7, 2015, doi:10.1038/nplants.2015.89.
APA
Ikeuchi, M., Iwase, A., Rymen, B., Harashima, H., Shibata, M., Ohnuma, M., … Sugimoto, K. (2015). PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis. NATURE PLANTS, 1(7). https://doi.org/10.1038/nplants.2015.89
Chicago author-date
Ikeuchi, Momoko, Akira Iwase, Bart Rymen, Hirofumi Harashima, Michitaro Shibata, Mariko Ohnuma, Christian Breuer, et al. 2015. “PRC2 Represses Dedifferentiation of Mature Somatic Cells in Arabidopsis.” NATURE PLANTS 1 (7). https://doi.org/10.1038/nplants.2015.89.
Chicago author-date (all authors)
Ikeuchi, Momoko, Akira Iwase, Bart Rymen, Hirofumi Harashima, Michitaro Shibata, Mariko Ohnuma, Christian Breuer, Ana Karina Morao, Miguel de Lucas, Lieven De Veylder, Justin Goodrich, Siobhan M Brady, François Roudier, and Keiko Sugimoto. 2015. “PRC2 Represses Dedifferentiation of Mature Somatic Cells in Arabidopsis.” NATURE PLANTS 1 (7). doi:10.1038/nplants.2015.89.
Vancouver
1.
Ikeuchi M, Iwase A, Rymen B, Harashima H, Shibata M, Ohnuma M, et al. PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis. NATURE PLANTS. 2015;1(7).
IEEE
[1]
M. Ikeuchi et al., “PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis,” NATURE PLANTS, vol. 1, no. 7, 2015.
@article{6917962,
  abstract     = {{Plant somatic cells are generally acknowledged to retain totipotency, the potential to develop into any cell type within an organism. This astonishing plasticity may contribute to a high regenerative capacity on severe damage, but how plants control this potential during normal post-embryonic development remains largely unknown1,2. Here we show that POLYCOMB REPRESSIVE COMPLEX 2 (PRC2), a chromatin regulator that maintains gene repression through histone modification, prevents dedifferentiation of mature somatic cells in Arabidopsis thaliana roots. Loss-of-function mutants in PRC2 subunits initially develop unicellular root hairs indistinguishable from those in wild type but fail to retain the differentiated state, ultimately resulting in the generation of an unorganized cell mass and somatic embryos from a single root hair. Strikingly, mutant root hairs complete the normal endoreduplication programme, increasing their nuclear ploidy, but subsequently reinitiate mitotic division coupled with successive DNA replication. Our data show that the WOUND INDUCED DEDIFFERENTIATION3 (WIND3) and LEAFY COTYLEDON2 (LEC2) genes are among the PRC2 targets involved in this reprogramming, as their ectopic overexpression partly phenocopies the dedifferentiation phenotype of PRC2 mutants. These findings unveil the pivotal role of PRC2-mediated gene repression in preventing unscheduled reprogramming of fully differentiated plant cells.}},
  articleno    = {{15089}},
  author       = {{Ikeuchi, Momoko and Iwase, Akira and Rymen, Bart and Harashima, Hirofumi and Shibata, Michitaro and Ohnuma, Mariko and Breuer, Christian and Morao, Ana Karina and de Lucas, Miguel and De Veylder, Lieven and Goodrich, Justin and Brady, Siobhan M and Roudier, François and Sugimoto, Keiko}},
  issn         = {{2055-026X}},
  journal      = {{NATURE PLANTS}},
  keywords     = {{MERISTEM,THALIANA,REGENERATION,ROOT,GROWTH,GENE-EXPRESSION,TRANSCRIPTION FACTOR,POLYCOMB-GROUP PROTEINS,TOPOISOMERASE-VI COMPLEX,PLANTS}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{7}},
  title        = {{PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis}},
  url          = {{http://dx.doi.org/10.1038/nplants.2015.89}},
  volume       = {{1}},
  year         = {{2015}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: