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V-ATPase-activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis

(2015) NATURE PLANTS. 1(7).
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Abstract
In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.
Keywords
CELLULOSE SYNTHASE COMPLEXES, VACUOLAR H+-ATPASE, ENDOMEMBRANE SYSTEM, PLASMA-MEMBRANE, GOLGI-APPARATUS, GENE-EXPRESSION, PLANT GOLGI, CELL, TRAFFICKING, REVEALS

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Chicago
Luo, Yu, Stefan Scholl, Anett Doering, Yi Zhang, Niloufer Irani, Simone Di Rubbo, Lutz Neumetzler, et al. 2015. “V-ATPase-activity in the TGN/EE Is Required for Exocytosis and Recycling in Arabidopsis.” Nature Plants 1 (7).
APA
Luo, Y., Scholl, S., Doering, A., Zhang, Y., Irani, N., Di Rubbo, S., Neumetzler, L., et al. (2015). V-ATPase-activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. NATURE PLANTS, 1(7).
Vancouver
1.
Luo Y, Scholl S, Doering A, Zhang Y, Irani N, Di Rubbo S, et al. V-ATPase-activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. NATURE PLANTS. 2015;1(7).
MLA
Luo, Yu, Stefan Scholl, Anett Doering, et al. “V-ATPase-activity in the TGN/EE Is Required for Exocytosis and Recycling in Arabidopsis.” NATURE PLANTS 1.7 (2015): n. pag. Print.
@article{6917932,
  abstract     = {In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.},
  articleno    = {15094},
  author       = {Luo, Yu and Scholl, Stefan and Doering, Anett and Zhang, Yi and Irani, Niloufer and Di Rubbo, Simone and Neumetzler, Lutz and Krisknamoorthy, Praveen and Vanhoutte, Isabelle and Mylle, Evelien and Bischoff, Volker and Vernhettes, Samantha and Winne, Johan and Friml, Jiri and Stierhof, York-Dieter and Schumacher, Karin and Persson, Staffan and Russinova, Eugenia},
  issn         = {2055-026X},
  journal      = {NATURE PLANTS},
  keyword      = {CELLULOSE SYNTHASE COMPLEXES,VACUOLAR H+-ATPASE,ENDOMEMBRANE SYSTEM,PLASMA-MEMBRANE,GOLGI-APPARATUS,GENE-EXPRESSION,PLANT GOLGI,CELL,TRAFFICKING,REVEALS},
  language     = {eng},
  number       = {7},
  pages        = {10},
  title        = {V-ATPase-activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis},
  url          = {http://dx.doi.org/10.1038/nplants.2015.94},
  volume       = {1},
  year         = {2015},
}

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