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An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana

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Abstract
Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin) is essential for plant development. Auxin gradient establishment is mediated by polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their localization and abundance at the plasma membrane are tightly regulated by endo-membrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF-defective mutants gnom-like 1 (gnl1-1) and gnom (van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. Our data confirm a role for GNOM in endoplasmic reticulum (ER)-Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential for normal plant development.
Keywords
CELL POLARITY, MEMBRANE TRAFFICKING, TRANSPORT INHIBITORS, PLANT DEVELOPMENT, ARF-GEF, NUCLEOTIDE-EXCHANGE FACTORS, AUXIN EFFLUX CARRIER, plasma membrane protein polarity, PIN auxin transporters, endomembrane trafficking, chemical genomics, ACTIN CYTOSKELETON, ENDOCYTIC PATHWAY, CHEMICAL GENOMICS

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MLA
Doyle, Siamsa M., et al. “An Early Secretory Pathway Mediated by GNOM-LIKE 1 and GNOM Is Essential for Basal Polarity Establishment in Arabidopsis Thaliana.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 112, no. 7, 2015, pp. E806–15, doi:10.1073/pnas.1424856112.
APA
Doyle, S. M., Haeger, A., Vain, T., Rigal, A., Viotti, C., Łangowska, M., … Robert, S. (2015). An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(7), E806–E815. https://doi.org/10.1073/pnas.1424856112
Chicago author-date
Doyle, Siamsa M, Ash Haeger, Thomas Vain, Adeline Rigal, Corrado Viotti, Malgorzata Łangowska, Qian Ma, et al. 2015. “An Early Secretory Pathway Mediated by GNOM-LIKE 1 and GNOM Is Essential for Basal Polarity Establishment in Arabidopsis Thaliana.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112 (7): E806–15. https://doi.org/10.1073/pnas.1424856112.
Chicago author-date (all authors)
Doyle, Siamsa M, Ash Haeger, Thomas Vain, Adeline Rigal, Corrado Viotti, Malgorzata Łangowska, Qian Ma, Jiri Friml, Natasha V Raikhel, Glenn R Hicks, and Stéphanie Robert. 2015. “An Early Secretory Pathway Mediated by GNOM-LIKE 1 and GNOM Is Essential for Basal Polarity Establishment in Arabidopsis Thaliana.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112 (7): E806–E815. doi:10.1073/pnas.1424856112.
Vancouver
1.
Doyle SM, Haeger A, Vain T, Rigal A, Viotti C, Łangowska M, et al. An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2015;112(7):E806–15.
IEEE
[1]
S. M. Doyle et al., “An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 112, no. 7, pp. E806–E815, 2015.
@article{6916502,
  abstract     = {{Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin) is essential for plant development. Auxin gradient establishment is mediated by polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their localization and abundance at the plasma membrane are tightly regulated by endo-membrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF-defective mutants gnom-like 1 (gnl1-1) and gnom (van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. Our data confirm a role for GNOM in endoplasmic reticulum (ER)-Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential for normal plant development.}},
  author       = {{Doyle, Siamsa M and Haeger, Ash and Vain, Thomas and Rigal, Adeline and Viotti, Corrado and Łangowska, Malgorzata and Ma, Qian and Friml, Jiri and Raikhel, Natasha V and Hicks, Glenn R and Robert, Stéphanie}},
  issn         = {{0027-8424}},
  journal      = {{PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}},
  keywords     = {{CELL POLARITY,MEMBRANE TRAFFICKING,TRANSPORT INHIBITORS,PLANT DEVELOPMENT,ARF-GEF,NUCLEOTIDE-EXCHANGE FACTORS,AUXIN EFFLUX CARRIER,plasma membrane protein polarity,PIN auxin transporters,endomembrane trafficking,chemical genomics,ACTIN CYTOSKELETON,ENDOCYTIC PATHWAY,CHEMICAL GENOMICS}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{E806--E815}},
  title        = {{An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana}},
  url          = {{http://dx.doi.org/10.1073/pnas.1424856112}},
  volume       = {{112}},
  year         = {{2015}},
}

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