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Improved analyte detectability of proteins and peptide lysates by means of multiple large-volume injection in LC-MS

(2009) JOURNAL OF SEPARATION SCIENCE. 32(14). p.2346-2352
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Abstract
A 'multiple (trapping) large-volume injection' approach was developed for the analysis of peptides and proteins. In this way, a maximally 10-fold gain in sensitivity could be achieved. The system involves the use of an automated 10-port switching valve in combination with a 1 mm i.d. trapping/guard column and a 1 mm i.d. x 150 mm analytical column. The optimized multiple injection/loading procedure allows quantitative measurements of peptides and protein lysates. Linear calibration curves (R(2) >= 0.988) over a minimum of two orders of magnitude were generated for a range of peptide and protein standards with sensitivities equal to or even exceeding, those generally achieved only through increasing miniaturization (quantification limit >= 0.5 pmol/mL).
Keywords
LIQUID-CHROMATOGRAPHY, COLUMN, TANDEM MASS-SPECTROMETRY, Large-volume injection, Peptides, Sensitivity, LC-MS(/MS), (Absolute) Protein quantitation, QUANTIFICATION

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Chicago
Storme, Michael, Ruben T’Kindt, and Jan Van Bocxlaer. 2009. “Improved Analyte Detectability of Proteins and Peptide Lysates by Means of Multiple Large-volume Injection in LC-MS.” Journal of Separation Science 32 (14): 2346–2352.
APA
Storme, Michael, T’Kindt, R., & Van Bocxlaer, J. (2009). Improved analyte detectability of proteins and peptide lysates by means of multiple large-volume injection in LC-MS. JOURNAL OF SEPARATION SCIENCE, 32(14), 2346–2352.
Vancouver
1.
Storme M, T’Kindt R, Van Bocxlaer J. Improved analyte detectability of proteins and peptide lysates by means of multiple large-volume injection in LC-MS. JOURNAL OF SEPARATION SCIENCE. 2009;32(14):2346–52.
MLA
Storme, Michael, Ruben T’Kindt, and Jan Van Bocxlaer. “Improved Analyte Detectability of Proteins and Peptide Lysates by Means of Multiple Large-volume Injection in LC-MS.” JOURNAL OF SEPARATION SCIENCE 32.14 (2009): 2346–2352. Print.
@article{6914691,
  abstract     = {A 'multiple (trapping) large-volume injection' approach was developed for the analysis of peptides and proteins. In this way, a maximally 10-fold gain in sensitivity could be achieved. The system involves the use of an automated 10-port switching valve in combination with a 1 mm i.d. trapping/guard column and a 1 mm i.d. x 150 mm analytical column. The optimized multiple injection/loading procedure allows quantitative measurements of peptides and protein lysates. Linear calibration curves (R(2) {\textrangle}= 0.988) over a minimum of two orders of magnitude were generated for a range of peptide and protein standards with sensitivities equal to or even exceeding, those generally achieved only through increasing miniaturization (quantification limit {\textrangle}= 0.5 pmol/mL).},
  author       = {Storme, Michael and T'Kindt, Ruben and Van Bocxlaer, Jan},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {LIQUID-CHROMATOGRAPHY,COLUMN,TANDEM MASS-SPECTROMETRY,Large-volume injection,Peptides,Sensitivity,LC-MS(/MS),(Absolute) Protein quantitation,QUANTIFICATION},
  language     = {eng},
  number       = {14},
  pages        = {2346--2352},
  title        = {Improved analyte detectability of proteins and peptide lysates by means of multiple large-volume injection in LC-MS},
  url          = {http://dx.doi.org/10.1002/jssc.200900131},
  volume       = {32},
  year         = {2009},
}

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