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Improved anti-inflammatory effects in rabbit eye model using biodegradable poly beta-amino ester nanoparticles of triamcinolone acetonide

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Abstract
PURPOSE. Results of previous studies on the benefits of ocular drug delivery using polymeric mucoadhesive nanoparticles suggested longer presence and better penetration of nanoparticles, and, thus, increased effect and bioavailability of drugs entrapped in nanoparticles. In this study, a novel polymer, poly beta-amino ester, was used for the preparation of triamcinolone acetonide-loaded nanoparticles using a modified emulsification/solvent diffusion method. METHODS. Mucoadhesiveness studies, in vitro drug release, x-ray powder diffraction, differential scanning calorimetry, and scanning electron microscopy were used for physicochemical characterization of nanoparticles. Thirty-six hours after inducing uveitis by intravitreal injection of a lipopolysaccharide, sampling from the aqueous humor was done and inflammatory factors, such as cell, protein, nitric oxide, and prostaglandin E2, were compared. RESULTS. Nanoparticles with a mean size of 178 nm and drug loading of 5.3% were prepared and used for in vivo studies in rabbits with uveitis. Higher anti-inflammatory effect was observed for polymeric nanoparticles of triamcinolone acetonide compared with microparticles of prednisolone acetate and triamcinolone acetonide, and an equal effect compared with subconjunctival injection of triamcinolone acetonide in terms of inhibiting inflammation and inflammatory mediators. CONCLUSIONS. It can be concluded that polymeric nanoparticles of triamcinolone acetonide will provide as good an anti-inflammatory effect as the subconjunctival injection method and are better compared with other drug delivery systems.
Keywords
uveitis, nitric oxide, mucoadhesive, poly beta-amino ester, nanoparticles, triamcinolone acetonide, ocular drug delivery, ENDOTOXIN-INDUCED UVEITIS, OCULAR DRUG-DELIVERY, NITRIC-OXIDE, POLY(BETA-AMINO ESTERS), IN-VITRO, CHITOSAN, SYSTEM, GENE, RATS, VIVO

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Chicago
Sabzevari, Araz, Khosro Adibkia, Hassan Hashemi, Bruno De Geest, Navid Mohsenzadeh, Fatemeh Atyabi, Mohammad Hossein Ghahremani, Mohammad-Reza Khoshayand, and Rassoul Dinarvand. 2013. “Improved Anti-inflammatory Effects in Rabbit Eye Model Using Biodegradable Poly Beta-amino Ester Nanoparticles of Triamcinolone Acetonide.” Investigative Ophthalmology & Visual Science 54 (8): 5520–5526.
APA
Sabzevari, A., Adibkia, K., Hashemi, H., De Geest, B., Mohsenzadeh, N., Atyabi, F., Ghahremani, M. H., et al. (2013). Improved anti-inflammatory effects in rabbit eye model using biodegradable poly beta-amino ester nanoparticles of triamcinolone acetonide. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 54(8), 5520–5526.
Vancouver
1.
Sabzevari A, Adibkia K, Hashemi H, De Geest B, Mohsenzadeh N, Atyabi F, et al. Improved anti-inflammatory effects in rabbit eye model using biodegradable poly beta-amino ester nanoparticles of triamcinolone acetonide. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 2013;54(8):5520–6.
MLA
Sabzevari, Araz, Khosro Adibkia, Hassan Hashemi, et al. “Improved Anti-inflammatory Effects in Rabbit Eye Model Using Biodegradable Poly Beta-amino Ester Nanoparticles of Triamcinolone Acetonide.” INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 54.8 (2013): 5520–5526. Print.
@article{6912937,
  abstract     = {PURPOSE. Results of previous studies on the benefits of ocular drug delivery using polymeric mucoadhesive nanoparticles suggested longer presence and better penetration of nanoparticles, and, thus, increased effect and bioavailability of drugs entrapped in nanoparticles. In this study, a novel polymer, poly beta-amino ester, was used for the preparation of triamcinolone acetonide-loaded nanoparticles using a modified emulsification/solvent diffusion method. 
METHODS. Mucoadhesiveness studies, in vitro drug release, x-ray powder diffraction, differential scanning calorimetry, and scanning electron microscopy were used for physicochemical characterization of nanoparticles. Thirty-six hours after inducing uveitis by intravitreal injection of a lipopolysaccharide, sampling from the aqueous humor was done and inflammatory factors, such as cell, protein, nitric oxide, and prostaglandin E2, were compared. 
RESULTS. Nanoparticles with a mean size of 178 nm and drug loading of 5.3\% were prepared and used for in vivo studies in rabbits with uveitis. Higher anti-inflammatory effect was observed for polymeric nanoparticles of triamcinolone acetonide compared with microparticles of prednisolone acetate and triamcinolone acetonide, and an equal effect compared with subconjunctival injection of triamcinolone acetonide in terms of inhibiting inflammation and inflammatory mediators. 
CONCLUSIONS. It can be concluded that polymeric nanoparticles of triamcinolone acetonide will provide as good an anti-inflammatory effect as the subconjunctival injection method and are better compared with other drug delivery systems.},
  author       = {Sabzevari, Araz and Adibkia, Khosro and Hashemi, Hassan and De Geest, Bruno and Mohsenzadeh, Navid and Atyabi, Fatemeh and Ghahremani, Mohammad Hossein and Khoshayand, Mohammad-Reza and Dinarvand, Rassoul},
  issn         = {0146-0404},
  journal      = {INVESTIGATIVE OPHTHALMOLOGY \& VISUAL SCIENCE},
  language     = {eng},
  number       = {8},
  pages        = {5520--5526},
  title        = {Improved anti-inflammatory effects in rabbit eye model using biodegradable poly beta-amino ester nanoparticles of triamcinolone acetonide},
  url          = {http://dx.doi.org/10.1167/iovs.13-12296},
  volume       = {54},
  year         = {2013},
}

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