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Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources

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Abstract
Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Boo L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9%, respectively) and towards established Bcc species (4.0 and 3.9%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156(T) (=CCUG 65686(T)) and Burkholderia territorii sp. nov. with type strain LMG 28158(T) (=CCUG 65687(T)) are proposed for Bcc B and Bcc L bacteria, respectively.
Keywords
TIME, TAXONOMY, EPIDEMIOLOGY, DNA

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Chicago
De Smet, Birgit, Mark Mayo, Charlotte Peeters, James EA Zlosnik, Theodore Spilker, Trevor J Hird, John J LiPuma, et al. 2015. “Burkholderia Stagnalis Sp. Nov. and Burkholderia Territorii Sp. Nov., Two Novel Burkholderia Cepacia Complex Species from Environmental and Human Sources.” International Journal of Systematic and Evolutionary Microbiology 65 (7): 2265–2271.
APA
De Smet, Birgit, Mayo, M., Peeters, C., Zlosnik, J. E., Spilker, T., Hird, T. J., LiPuma, J. J., et al. (2015). Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 65(7), 2265–2271.
Vancouver
1.
De Smet B, Mayo M, Peeters C, Zlosnik JE, Spilker T, Hird TJ, et al. Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2015;65(7):2265–71.
MLA
De Smet, Birgit, Mark Mayo, Charlotte Peeters, et al. “Burkholderia Stagnalis Sp. Nov. and Burkholderia Territorii Sp. Nov., Two Novel Burkholderia Cepacia Complex Species from Environmental and Human Sources.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65.7 (2015): 2265–2271. Print.
@article{6912054,
  abstract     = {Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Boo L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9\%, respectively) and towards established Bcc species (4.0 and 3.9\%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156(T) (=CCUG 65686(T)) and Burkholderia territorii sp. nov. with type strain LMG 28158(T) (=CCUG 65687(T)) are proposed for Bcc B and Bcc L bacteria, respectively.},
  author       = {De Smet, Birgit and Mayo, Mark and Peeters, Charlotte and Zlosnik, James EA and Spilker, Theodore and Hird, Trevor J and LiPuma, John J and Kidd, Timothy J and Kaestli, Mirjam and Ginther, Jennifer L and Wagner, David M and Keim, Paul and Bell, Scott C and Jacobs, Jan A and Currie, Bart J and Vandamme, Peter},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {TIME,TAXONOMY,EPIDEMIOLOGY,DNA},
  language     = {eng},
  number       = {7},
  pages        = {2265--2271},
  title        = {Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources},
  url          = {http://dx.doi.org/10.1099/ijs.0.000251},
  volume       = {65},
  year         = {2015},
}

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