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PtaRHE1, a Populus tremula x Populus alba RING-H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development

(2015) PLANT JOURNAL. 82(6). p.978-990
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01MR05
Abstract
REALLY INTERESTING NEW GENE (RING) proteins play important roles in the regulation of many processes by recognizing target proteins for ubiquitination. Previously, we have shown that the expression of PtaRHE1, encoding a Populus tremulaxPopulus alba RING-H2 protein with E3 ubiquitin ligase activity, is associated with tissues undergoing secondary growth. To further elucidate the role of PtaRHE1 in vascular tissues, we have undertaken a reverse genetic analysis in poplar. Within stem secondary vascular tissues, PtaRHE1 and its corresponding protein are expressed predominantly in the phloem. The downregulation of PtaRHE1 in poplar by artificial miRNA triggers alterations in phloem fibre patterning, characterized by an increased portion of secondary phloem fibres that have a reduced cell wall thickness and a change in lignin composition, with lower levels of syringyl units as compared with wild-type plants. Following an RNA-seq analysis, a biological network involving hormone stress signalling, as well as developmental processes, could be delineated. Several candidate genes possibly associated with the altered phloem fibre phenotype observed in amiRPtaRHE1 poplar were identified. Altogether, our data suggest a regulatory role for PtaRHE1 in secondary phloem fibre development. Significance Statement In poplar, as secondary growth proceeds in stem, PtaRHE1 expression is mainly restricted to secondary phloem tissue where the corresponding protein may function as an E3 ligase to control differentiation of parenchyma cells in secondary phloem fibers within the bark.
Keywords
IDENTIFICATION, LIGNIN, GROWTH, EXPRESSION, GENE, ARABIDOPSIS-THALIANA, TRANSCRIPTION FACTOR, UBIQUITIN LIGASES, CARBOHYDRATE-ACTIVE ENZYMES, Populus tremulaxPopulus alba, phloem fibre development, TREES, vascular tissues, PtaRHE1, RING-H2

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Citation

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Chicago
Baldacci-Cresp, Fabien, Jihad Moussawi, Jean-Charles Leplé, Rebecca Van Acker, Annegret Kohler, Julie Candiracci, Laure Twyffels, et al. 2015. “PtaRHE1, a Populus Tremula x Populus Alba RING-H2 Protein of the ATL Family, Has a Regulatory Role in Secondary Phloem Fibre Development.” Plant Journal 82 (6): 978–990.
APA
Baldacci-Cresp, F., Moussawi, J., Leplé, J.-C., Van Acker, R., Kohler, A., Candiracci, J., Twyffels, L., et al. (2015). PtaRHE1, a Populus tremula x Populus alba RING-H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development. PLANT JOURNAL, 82(6), 978–990.
Vancouver
1.
Baldacci-Cresp F, Moussawi J, Leplé J-C, Van Acker R, Kohler A, Candiracci J, et al. PtaRHE1, a Populus tremula x Populus alba RING-H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development. PLANT JOURNAL. 2015;82(6):978–90.
MLA
Baldacci-Cresp, Fabien, Jihad Moussawi, Jean-Charles Leplé, et al. “PtaRHE1, a Populus Tremula x Populus Alba RING-H2 Protein of the ATL Family, Has a Regulatory Role in Secondary Phloem Fibre Development.” PLANT JOURNAL 82.6 (2015): 978–990. Print.
@article{6908199,
  abstract     = {REALLY INTERESTING NEW GENE (RING) proteins play important roles in the regulation of many processes by recognizing target proteins for ubiquitination. Previously, we have shown that the expression of PtaRHE1, encoding a Populus tremulaxPopulus alba RING-H2 protein with E3 ubiquitin ligase activity, is associated with tissues undergoing secondary growth. To further elucidate the role of PtaRHE1 in vascular tissues, we have undertaken a reverse genetic analysis in poplar. Within stem secondary vascular tissues, PtaRHE1 and its corresponding protein are expressed predominantly in the phloem. The downregulation of PtaRHE1 in poplar by artificial miRNA triggers alterations in phloem fibre patterning, characterized by an increased portion of secondary phloem fibres that have a reduced cell wall thickness and a change in lignin composition, with lower levels of syringyl units as compared with wild-type plants. Following an RNA-seq analysis, a biological network involving hormone stress signalling, as well as developmental processes, could be delineated. Several candidate genes possibly associated with the altered phloem fibre phenotype observed in amiRPtaRHE1 poplar were identified. Altogether, our data suggest a regulatory role for PtaRHE1 in secondary phloem fibre development. 
Significance Statement In poplar, as secondary growth proceeds in stem, PtaRHE1 expression is mainly restricted to secondary phloem tissue where the corresponding protein may function as an E3 ligase to control differentiation of parenchyma cells in secondary phloem fibers within the bark.},
  author       = {Baldacci-Cresp, Fabien and Moussawi, Jihad and Lepl{\'e}, Jean-Charles and Van Acker, Rebecca and Kohler, Annegret and Candiracci, Julie and Twyffels, Laure and Spokevicius, Antanas V and Bossinger, Gerd and Laurans, Fran\c{c}oise and Brunel, Nicole and Vermeersch, Marjorie and Boerjan, Wout and El Jaziri, Mondher and Baucher, Maria},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  language     = {eng},
  number       = {6},
  pages        = {978--990},
  title        = {PtaRHE1, a Populus tremula x Populus alba RING-H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development},
  url          = {http://dx.doi.org/10.1111/tpj.12867},
  volume       = {82},
  year         = {2015},
}

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