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Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25–3T studied in an in vitro gut model

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Abstract
Butyrate-producing bacteria are promising probiotic candidates to target microbial dysbiosis in gastrointestinal disorders like inflammatory bowel diseases. Butyricicoccus pullicaecorum 25-3(T), a butyrate-producing clostridial cluster IV strain, is such a candidate. Little is known about its abundance in the colon microbiota and its butyrogenic properties. We used the M-SHIME (R), an in vitro simulator for the human intestinal microbial ecosystem, to study the effect of supplementing a single dose of B. pullicaecorum 25-3(T) on lumen-and mucus-associated microbiota of eight individuals. Butyricicoccus pullicaecorum was more abundant in mucus-associated microbiota compared with lumen microbiota. Supplementation with a single dose of B. pullicaecorum 25-3(T) resulted in a temporary increase in B. pullicaecorum bacteria in lumen compartment of all individuals. In two cases, the responders, an increased butyrate production was observed as compared with the control. 16S rRNA gene amplicon sequencing revealed the microbiota of responders to be different as compared to non-responder microbiota. We can conclude that B. pullicaecorum 25-3(T) is a mucus-associated bacterium whose potency to stimulate butyrate production is characterized by a large interindividual variability in terms of composition of the receiving microbial community.
Keywords
SULFATE-INDUCED COLITIS, BUTYRIVIBRIO-FIBRISOLVENS, INFLAMMATORY-BOWEL-DISEASE, anaerobe, ULCERATIVE-COLITIS, DIVERSITY, COLONIZATION, INTESTINE, GEN. NOV., probiotic, colonization, gastrointestinal microbial ecology, in vitro, RIBOSOMAL-RNA, butyric acid, FECAL MICROBIOTA

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Chicago
Geirnaert, Annelies, Jun Wang, Magali Tinck, Alix Steyaert, Pieter Van den Abbeele, Venessa Eeckhaut, Ramiro Vilchez Vargas, et al. 2015. “Interindividual Differences in Response to Treatment with Butyrate-producing Butyricicoccus Pullicaecorum 25–3T Studied in an in Vitro Gut Model.” Fems Microbiology Ecology 91 (6).
APA
Geirnaert, A., Wang, J., Tinck, M., Steyaert, A., Van den Abbeele, P., Eeckhaut, V., Vilchez Vargas, R., et al. (2015). Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25–3T studied in an in vitro gut model. FEMS MICROBIOLOGY ECOLOGY, 91(6).
Vancouver
1.
Geirnaert A, Wang J, Tinck M, Steyaert A, Van den Abbeele P, Eeckhaut V, et al. Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25–3T studied in an in vitro gut model. FEMS MICROBIOLOGY ECOLOGY. 2015;91(6).
MLA
Geirnaert, Annelies, Jun Wang, Magali Tinck, et al. “Interindividual Differences in Response to Treatment with Butyrate-producing Butyricicoccus Pullicaecorum 25–3T Studied in an in Vitro Gut Model.” FEMS MICROBIOLOGY ECOLOGY 91.6 (2015): n. pag. Print.
@article{6906372,
  abstract     = {Butyrate-producing bacteria are promising probiotic candidates to target microbial dysbiosis in gastrointestinal disorders like inflammatory bowel diseases. Butyricicoccus pullicaecorum 25-3(T), a butyrate-producing clostridial cluster IV strain, is such a candidate. Little is known about its abundance in the colon microbiota and its butyrogenic properties. We used the M-SHIME (R), an in vitro simulator for the human intestinal microbial ecosystem, to study the effect of supplementing a single dose of B. pullicaecorum 25-3(T) on lumen-and mucus-associated microbiota of eight individuals. Butyricicoccus pullicaecorum was more abundant in mucus-associated microbiota compared with lumen microbiota. Supplementation with a single dose of B. pullicaecorum 25-3(T) resulted in a temporary increase in B. pullicaecorum bacteria in lumen compartment of all individuals. In two cases, the responders, an increased butyrate production was observed as compared with the control. 16S rRNA gene amplicon sequencing revealed the microbiota of responders to be different as compared to non-responder microbiota. We can conclude that B. pullicaecorum 25-3(T) is a mucus-associated bacterium whose potency to stimulate butyrate production is characterized by a large interindividual variability in terms of composition of the receiving microbial community.},
  articleno    = {fiv054},
  author       = {Geirnaert, Annelies and Wang, Jun and Tinck, Magali and Steyaert, Alix and Van den Abbeele, Pieter and Eeckhaut, Venessa and Vilchez Vargas, Ramiro and Falony, Gwen and Laukens, Debby and De Vos, Martine and Van Immerseel, Filip and Raes, Jeroen and Boon, Nico and Van de Wiele, Tom},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  language     = {eng},
  number       = {6},
  pages        = {12},
  title        = {Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25--3T studied in an in vitro gut model},
  url          = {http://dx.doi.org/10.1093/femsec/fiv054},
  volume       = {91},
  year         = {2015},
}

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