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Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture

(2014) PLOS ONE. 9(11).
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Abstract
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/ progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34(+)-enriched populations of PB and BM, CD34(+)-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34(+)-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.
Keywords
IN-VITRO CULTURE, RED-BLOOD-CELLS, STEM-CELLS, BONE-MARROW, CORD-BLOOD, PERIPHERAL-BLOOD, MALARIA, INFECTION, INVASION

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MLA
Noulin, Florian et al. “Hematopoietic Stem/progenitor Cell Sources to Generate Reticulocytes for Plasmodium Vivax Culture.” PLOS ONE 9.11 (2014): n. pag. Print.
APA
Noulin, F., Manesia, J. K., Rosanas-Urgell, A., Erhart, A., Borlon, C., Van Den Abbeele, J., d’ Alessandro, U., et al. (2014). Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. PLOS ONE, 9(11).
Chicago author-date
Noulin, Florian, Javed Karim Manesia, Anna Rosanas-Urgell, Annette Erhart, Céline Borlon, Jan Van Den Abbeele, Umberto d’ Alessandro, and Catherine M Verfaillie. 2014. “Hematopoietic Stem/progenitor Cell Sources to Generate Reticulocytes for Plasmodium Vivax Culture.” Plos One 9 (11).
Chicago author-date (all authors)
Noulin, Florian, Javed Karim Manesia, Anna Rosanas-Urgell, Annette Erhart, Céline Borlon, Jan Van Den Abbeele, Umberto d’ Alessandro, and Catherine M Verfaillie. 2014. “Hematopoietic Stem/progenitor Cell Sources to Generate Reticulocytes for Plasmodium Vivax Culture.” Plos One 9 (11).
Vancouver
1.
Noulin F, Manesia JK, Rosanas-Urgell A, Erhart A, Borlon C, Van Den Abbeele J, et al. Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. PLOS ONE. 2014;9(11).
IEEE
[1]
F. Noulin et al., “Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture,” PLOS ONE, vol. 9, no. 11, 2014.
@article{6905576,
  abstract     = {{The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/ progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34(+)-enriched populations of PB and BM, CD34(+)-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34(+)-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.}},
  articleno    = {{e112496}},
  author       = {{Noulin, Florian and Manesia, Javed Karim and Rosanas-Urgell, Anna and Erhart, Annette and Borlon, Céline and Van Den Abbeele, Jan and d'Alessandro, Umberto and Verfaillie, Catherine M}},
  issn         = {{1932-6203}},
  journal      = {{PLOS ONE}},
  keywords     = {{IN-VITRO CULTURE,RED-BLOOD-CELLS,STEM-CELLS,BONE-MARROW,CORD-BLOOD,PERIPHERAL-BLOOD,MALARIA,INFECTION,INVASION}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{7}},
  title        = {{Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0112496}},
  volume       = {{9}},
  year         = {{2014}},
}

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