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Serological diagnosis of bovine neosporosis: a Bayesian evaluation of two antibody ELISA tests for in vivo diagnosis in purchased and abortion cattle

(2015) VETERINARY RECORD. 176(23).
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Abstract
Currently, there are no perfect reference tests for the in vivo detection of Neospora caninum infection. Two commercial N caninum ELISA tests are currently used in Belgium for bovine sera (TEST A and TEST B). The goal of this study is to evaluate these tests used at their current cut-offs, with a no gold standard approach, for the test purpose of (1) demonstration of freedom of infection at purchase and (2) diagnosis in aborting cattle. Sera of two study populations, Abortion population (n=196) and Purchase population (n=514), were selected and tested with both ELISA's. Test results were entered in a Bayesian model with informative priors on population prevalences only (Scenario 1). As sensitivity analysis, two more models were used: one with informative priors on test diagnostic accuracy (Scenario 2) and one with all priors uninformative (Scenario 3). The accuracy parameters were estimated from the first model: diagnostic sensitivity (Test A: 93.54 per cent-Test B: 86.99 per cent) and specificity (Test A: 90.22 per cent-Test B: 90.15 per cent) were high and comparable (Bayesian P values > 0.05). Based on predictive values in the two study populations, both tests were fit for purpose, despite an expected false negative fraction of +/- 0.5 per cent in the Purchase population and +/- 5 per cent in the Abortion population. In addition, a false positive fraction of +/- 3 per cent in the overall Purchase population and +/- 4 per cent in the overall Abortion population was found.
Keywords
SPECIFICITY, PARATUBERCULOSIS, SENSITIVITY, VALIDATION, ACCURACY, PCR, DAIRY, BLOOD, CONDITIONAL DEPENDENCE, CANINUM INFECTION

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Chicago
Roelandt, S, Yves Van der Stede, G Czaplicki, H Van Loo, E Van Driessche, Jeroen Dewulf, J Hooyberghs, and C Faes. 2015. “Serological Diagnosis of Bovine Neosporosis: a Bayesian Evaluation of Two Antibody ELISA Tests for in Vivo Diagnosis in Purchased and Abortion Cattle.” Veterinary Record 176 (23).
APA
Roelandt, S, Van der Stede, Y., Czaplicki, G., Van Loo, H., Van Driessche, E., Dewulf, J., Hooyberghs, J., et al. (2015). Serological diagnosis of bovine neosporosis: a Bayesian evaluation of two antibody ELISA tests for in vivo diagnosis in purchased and abortion cattle. VETERINARY RECORD, 176(23).
Vancouver
1.
Roelandt S, Van der Stede Y, Czaplicki G, Van Loo H, Van Driessche E, Dewulf J, et al. Serological diagnosis of bovine neosporosis: a Bayesian evaluation of two antibody ELISA tests for in vivo diagnosis in purchased and abortion cattle. VETERINARY RECORD. 2015;176(23).
MLA
Roelandt, S et al. “Serological Diagnosis of Bovine Neosporosis: a Bayesian Evaluation of Two Antibody ELISA Tests for in Vivo Diagnosis in Purchased and Abortion Cattle.” VETERINARY RECORD 176.23 (2015): n. pag. Print.
@article{6900986,
  abstract     = {Currently, there are no perfect reference tests for the in vivo detection of Neospora caninum infection. Two commercial N caninum ELISA tests are currently used in Belgium for bovine sera (TEST A and TEST B). The goal of this study is to evaluate these tests used at their current cut-offs, with a no gold standard approach, for the test purpose of (1) demonstration of freedom of infection at purchase and (2) diagnosis in aborting cattle. Sera of two study populations, Abortion population (n=196) and Purchase population (n=514), were selected and tested with both ELISA's. Test results were entered in a Bayesian model with informative priors on population prevalences only (Scenario 1). As sensitivity analysis, two more models were used: one with informative priors on test diagnostic accuracy (Scenario 2) and one with all priors uninformative (Scenario 3). The accuracy parameters were estimated from the first model: diagnostic sensitivity (Test A: 93.54 per cent-Test B: 86.99 per cent) and specificity (Test A: 90.22 per cent-Test B: 90.15 per cent) were high and comparable (Bayesian P values > 0.05). Based on predictive values in the two study populations, both tests were fit for purpose, despite an expected false negative fraction of +/- 0.5 per cent in the Purchase population and +/- 5 per cent in the Abortion population. In addition, a false positive fraction of +/- 3 per cent in the overall Purchase population and +/- 4 per cent in the overall Abortion population was found.},
  articleno    = {598},
  author       = {Roelandt, S and Van der Stede, Yves and Czaplicki, G and Van Loo, H and Van Driessche, E and Dewulf, Jeroen and Hooyberghs, J and Faes, C},
  issn         = {0042-4900},
  journal      = {VETERINARY RECORD},
  keywords     = {SPECIFICITY,PARATUBERCULOSIS,SENSITIVITY,VALIDATION,ACCURACY,PCR,DAIRY,BLOOD,CONDITIONAL DEPENDENCE,CANINUM INFECTION},
  language     = {eng},
  number       = {23},
  pages        = {9},
  title        = {Serological diagnosis of bovine neosporosis: a Bayesian evaluation of two antibody ELISA tests for in vivo diagnosis in purchased and abortion cattle},
  url          = {http://dx.doi.org/10.1136/vr.102872},
  volume       = {176},
  year         = {2015},
}

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