Advanced search
1 file | 433.80 KB Add to list

Milk prolactin response and quarter milk yield after experimental infection with coagulase-negative staphylococci in dairy heifers

(2015) JOURNAL OF DAIRY SCIENCE. 98(7). p.4593-4600
Author
Organization
Abstract
Coagulase-negative staphylococci (CNS) are the most common bacteria involved in subclinical mastitis in dairy cows. Remarkably, CNS-infected dairy heifers produce more milk than uninfected heifers. Because the lactation hormone prolactin (PRL) is also involved in mammary gland immunity, we investigated the milk PRL response and the mammary quarter milk yield following experimental CNS challenge. Eight healthy Holstein-Friesian heifers in mid-lactation were experimentally infected using a split-udder design with 3 different CNS strains: one Staphylococcus fleurettii (from sawdust bedding) and 2 Staphylococcus chromogenes strains (one isolate from a teat apex, the other isolate from a chronic intramammary infection). Three mammary quarters per heifer were simultaneously inoculated with 1.0 x 10(6) cfu, whereas the remaining mammary quarter was infused with sterile phosphate-buffered saline, serving as a control. An existing radioimmunoassay was modified, validated, and used to measure PRL frozen-thawed milk at various time points until 78 h after challenge. The mean milk PRL level tended to be higher in the CNS-challenged mammary quarters compared with the control mammary quarters (7.56 and 6.85 ng/mL, respectively). The increase in PRL over time was significantly greater in the CNS-challenged mammary quarters than in the control mammary quarters. However, no difference was found in the PRL response when comparing each individual CNS strain with the control mammary quarters. The mean mammary quarter milk yield tended to be lower in the CNS-infected mammary quarters than in the control mammary quarters (1.73 and 1.98 kg per milking, respectively). The greatest milk loss occurred in the mammary quarters challenged with the intramammary strain of S. chromogenes. Future observational studies are needed to elucidate the relation between PRL, the milk yield, and the inflammatory condition, or infection status, of the mammary gland.
Keywords
dairy heifer, experimental mastitis, coagulase-negative staphylococci, CLINICAL MASTITIS, EARLY LACTATION, GROWTH-HORMONE, 1ST LACTATION, BOVINE MASTITIS, prolactin, MAMMARY EPITHELIAL-CELLS, MAJOR MASTITIS PATHOGENS, RECEPTOR, COWS, EPIDEMIOLOGY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 433.80 KB

Citation

Please use this url to cite or link to this publication:

MLA
Piccart, Kristine et al. “Milk Prolactin Response and Quarter Milk Yield After Experimental Infection with Coagulase-negative Staphylococci in Dairy Heifers.” JOURNAL OF DAIRY SCIENCE 98.7 (2015): 4593–4600. Print.
APA
Piccart, K., Piepers, S., Verbeke, J., de Sousa, N., Beckers, J., & De Vliegher, S. (2015). Milk prolactin response and quarter milk yield after experimental infection with coagulase-negative staphylococci in dairy heifers. JOURNAL OF DAIRY SCIENCE, 98(7), 4593–4600.
Chicago author-date
Piccart, Kristine, Sofie Piepers, Joren Verbeke, NM de Sousa, JF Beckers, and Sarne De Vliegher. 2015. “Milk Prolactin Response and Quarter Milk Yield After Experimental Infection with Coagulase-negative Staphylococci in Dairy Heifers.” Journal of Dairy Science 98 (7): 4593–4600.
Chicago author-date (all authors)
Piccart, Kristine, Sofie Piepers, Joren Verbeke, NM de Sousa, JF Beckers, and Sarne De Vliegher. 2015. “Milk Prolactin Response and Quarter Milk Yield After Experimental Infection with Coagulase-negative Staphylococci in Dairy Heifers.” Journal of Dairy Science 98 (7): 4593–4600.
Vancouver
1.
Piccart K, Piepers S, Verbeke J, de Sousa N, Beckers J, De Vliegher S. Milk prolactin response and quarter milk yield after experimental infection with coagulase-negative staphylococci in dairy heifers. JOURNAL OF DAIRY SCIENCE. 2015;98(7):4593–600.
IEEE
[1]
K. Piccart, S. Piepers, J. Verbeke, N. de Sousa, J. Beckers, and S. De Vliegher, “Milk prolactin response and quarter milk yield after experimental infection with coagulase-negative staphylococci in dairy heifers,” JOURNAL OF DAIRY SCIENCE, vol. 98, no. 7, pp. 4593–4600, 2015.
@article{6900517,
  abstract     = {Coagulase-negative staphylococci (CNS) are the most common bacteria involved in subclinical mastitis in dairy cows. Remarkably, CNS-infected dairy heifers produce more milk than uninfected heifers. Because the lactation hormone prolactin (PRL) is also involved in mammary gland immunity, we investigated the milk PRL response and the mammary quarter milk yield following experimental CNS challenge. Eight healthy Holstein-Friesian heifers in mid-lactation were experimentally infected using a split-udder design with 3 different CNS strains: one Staphylococcus fleurettii (from sawdust bedding) and 2 Staphylococcus chromogenes strains (one isolate from a teat apex, the other isolate from a chronic intramammary infection). Three mammary quarters per heifer were simultaneously inoculated with 1.0 x 10(6) cfu, whereas the remaining mammary quarter was infused with sterile phosphate-buffered saline, serving as a control. An existing radioimmunoassay was modified, validated, and used to measure PRL frozen-thawed milk at various time points until 78 h after challenge. The mean milk PRL level tended to be higher in the CNS-challenged mammary quarters compared with the control mammary quarters (7.56 and 6.85 ng/mL, respectively). The increase in PRL over time was significantly greater in the CNS-challenged mammary quarters than in the control mammary quarters. However, no difference was found in the PRL response when comparing each individual CNS strain with the control mammary quarters. The mean mammary quarter milk yield tended to be lower in the CNS-infected mammary quarters than in the control mammary quarters (1.73 and 1.98 kg per milking, respectively). The greatest milk loss occurred in the mammary quarters challenged with the intramammary strain of S. chromogenes. Future observational studies are needed to elucidate the relation between PRL, the milk yield, and the inflammatory condition, or infection status, of the mammary gland.},
  author       = {Piccart, Kristine and Piepers, Sofie and Verbeke, Joren and de Sousa, NM and Beckers, JF and De Vliegher, Sarne},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {dairy heifer,experimental mastitis,coagulase-negative staphylococci,CLINICAL MASTITIS,EARLY LACTATION,GROWTH-HORMONE,1ST LACTATION,BOVINE MASTITIS,prolactin,MAMMARY EPITHELIAL-CELLS,MAJOR MASTITIS PATHOGENS,RECEPTOR,COWS,EPIDEMIOLOGY},
  language     = {eng},
  number       = {7},
  pages        = {4593--4600},
  title        = {Milk prolactin response and quarter milk yield after experimental infection with coagulase-negative staphylococci in dairy heifers},
  url          = {http://dx.doi.org/10.3168/jds.2015-9349},
  volume       = {98},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: