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Dynamic changes in ANGUSTIFOLIA3 complex composition reveal a growth regulatory mechanism in the maize leaf

Hilde Nelissen (UGent) , Dominique Eeckhout (UGent) , Kirin Demuynck (UGent) , Geert Persiau (UGent) , Alan Walton (UGent) , Michiel Van Bel (UGent) , Marieke Vervoort, Jasper Candaele (UGent) , Jolien De Block (UGent) , Stijn Aesaert (UGent) , et al.
(2015) PLANT CELL. 27(6). p.1605-1619
Author
Organization
Project
Bioinformatics: from nucleotids to networks (N2N)
Project
Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Most molecular processes during plant development occur with a particular spatio-temporal specificity. Thus far, it has remained technically challenging to capture dynamic protein-protein interactions within a growing organ, where the interplay between cell division and cell expansion is instrumental. Here, we combined high-resolution sampling of the growing maize (Zea mays) leaf with tandem affinity purification followed by mass spectrometry. Our results indicate that the growth-regulating SWI/SNF chromatin remodeling complex associated with ANGUSTIFOLIA3 (AN3) was conserved within growing organs and between dicots and monocots. Moreover, we were able to demonstrate the dynamics of the AN3-interacting proteins within the growing leaf, since copurified GROWTH-REGULATING FACTORs (GRFs) varied throughout the growing leaf. Indeed, GRF1, GRF6, GRF7, GRF12, GRF15, and GRF17 were significantly enriched in the division zone of the growing leaf, while GRF4 and GRF10 levels were comparable between division zone and expansion zone in the growing leaf. These dynamics were also reflected at the mRNA and protein levels, indicating tight developmental regulation of the AN3-associated chromatin remodeling complex. In addition, the phenotypes of maize plants overexpressing miRNA396a-resistant GRF1 support a model proposing that distinct associations of the chromatin remodeling complex with specific GRFs tightly regulate the transition between cell division and cell expansion. Together, our data demonstrate that advancing from static to dynamic protein-protein interaction analysis in a growing organ adds insights in how developmental switches are regulated.
Keywords
GRF TRANSCRIPTION FACTORS, ARABIDOPSIS-THALIANA, CELL-DIVISION, PROTEIN COMPLEXES, MICRORNA MIR396, GENE, DIFFERENTIATION, FAMILY, TANDEM AFFINITY PURIFICATION, CHROMATIN REMODELING COMPLEXES

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Citation

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MLA
Nelissen, Hilde et al. “Dynamic Changes in ANGUSTIFOLIA3 Complex Composition Reveal a Growth Regulatory Mechanism in the Maize Leaf.” PLANT CELL 27.6 (2015): 1605–1619. Print.
APA
Nelissen, H., Eeckhout, D., Demuynck, K., Persiau, G., Walton, A., Van Bel, M., Vervoort, M., et al. (2015). Dynamic changes in ANGUSTIFOLIA3 complex composition reveal a growth regulatory mechanism in the maize leaf. PLANT CELL, 27(6), 1605–1619.
Chicago author-date
Nelissen, Hilde, Dominique Eeckhout, Kirin Demuynck, Geert Persiau, Alan Walton, Michiel Van Bel, Marieke Vervoort, et al. 2015. “Dynamic Changes in ANGUSTIFOLIA3 Complex Composition Reveal a Growth Regulatory Mechanism in the Maize Leaf.” Plant Cell 27 (6): 1605–1619.
Chicago author-date (all authors)
Nelissen, Hilde, Dominique Eeckhout, Kirin Demuynck, Geert Persiau, Alan Walton, Michiel Van Bel, Marieke Vervoort, Jasper Candaele, Jolien De Block, Stijn Aesaert, Maria Van Lijsebettens, Sofie Goormachtig, Klaas Vandepoele, Jelle Van Leene, Michael Muszynski, Kris Gevaert, Dirk Inzé, and Geert De Jaeger. 2015. “Dynamic Changes in ANGUSTIFOLIA3 Complex Composition Reveal a Growth Regulatory Mechanism in the Maize Leaf.” Plant Cell 27 (6): 1605–1619.
Vancouver
1.
Nelissen H, Eeckhout D, Demuynck K, Persiau G, Walton A, Van Bel M, et al. Dynamic changes in ANGUSTIFOLIA3 complex composition reveal a growth regulatory mechanism in the maize leaf. PLANT CELL. 2015;27(6):1605–19.
IEEE
[1]
H. Nelissen et al., “Dynamic changes in ANGUSTIFOLIA3 complex composition reveal a growth regulatory mechanism in the maize leaf,” PLANT CELL, vol. 27, no. 6, pp. 1605–1619, 2015.
@article{6890910,
  abstract     = {Most molecular processes during plant development occur with a particular spatio-temporal specificity. Thus far, it has remained technically challenging to capture dynamic protein-protein interactions within a growing organ, where the interplay between cell division and cell expansion is instrumental. Here, we combined high-resolution sampling of the growing maize (Zea mays) leaf with tandem affinity purification followed by mass spectrometry. Our results indicate that the growth-regulating SWI/SNF chromatin remodeling complex associated with ANGUSTIFOLIA3 (AN3) was conserved within growing organs and between dicots and monocots. Moreover, we were able to demonstrate the dynamics of the AN3-interacting proteins within the growing leaf, since copurified GROWTH-REGULATING FACTORs (GRFs) varied throughout the growing leaf. Indeed, GRF1, GRF6, GRF7, GRF12, GRF15, and GRF17 were significantly enriched in the division zone of the growing leaf, while GRF4 and GRF10 levels were comparable between division zone and expansion zone in the growing leaf. These dynamics were also reflected at the mRNA and protein levels, indicating tight developmental regulation of the AN3-associated chromatin remodeling complex. In addition, the phenotypes of maize plants overexpressing miRNA396a-resistant GRF1 support a model proposing that distinct associations of the chromatin remodeling complex with specific GRFs tightly regulate the transition between cell division and cell expansion. Together, our data demonstrate that advancing from static to dynamic protein-protein interaction analysis in a growing organ adds insights in how developmental switches are regulated.},
  author       = {Nelissen, Hilde and Eeckhout, Dominique and Demuynck, Kirin and Persiau, Geert and Walton, Alan and Van Bel, Michiel and Vervoort, Marieke and Candaele, Jasper and De Block, Jolien and Aesaert, Stijn and Van Lijsebettens, Maria and Goormachtig, Sofie and Vandepoele, Klaas and Van Leene, Jelle and Muszynski, Michael and Gevaert, Kris and Inzé, Dirk and De Jaeger, Geert},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keywords     = {GRF TRANSCRIPTION FACTORS,ARABIDOPSIS-THALIANA,CELL-DIVISION,PROTEIN COMPLEXES,MICRORNA MIR396,GENE,DIFFERENTIATION,FAMILY,TANDEM AFFINITY PURIFICATION,CHROMATIN REMODELING COMPLEXES},
  language     = {eng},
  number       = {6},
  pages        = {1605--1619},
  title        = {Dynamic changes in ANGUSTIFOLIA3 complex composition reveal a growth regulatory mechanism in the maize leaf},
  url          = {http://dx.doi.org/10.1105/tpc.15.00269},
  volume       = {27},
  year         = {2015},
}

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