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Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells

Henk Joos, Benedikt Timmerman, Marc Van Montagu UGent and Jeff Schell (1983) EMBO JOURNAL. 2(12). p.2151-2160
abstract
In an attempt to elucidate the transfer and integration mechanism of Agrobacterium DNA upon crown gall induction, we translocated a borderless T-DNA to different sites of the C58 Ti plasmid. As a result of the physical linkage of the T-DNA onc genes with other Ti plasmid functions, the concerned strain retained tumor-inducing capacity. However, when the borderless T-DNA is separated on an independent replicon while all other pTi functions are provided in trans, the strain can no longer induce tumors on plants. We provide evidence that the right T-DNA border region harbors one or more in cis active functions essential in the transfer and/or stabilization of the T-DNA into plant cells. The strains used in these experiments allowed us to conclude that some function(s) of the Ti plasmid can induce plant cell proliferations independently of the T-DNA transformation event. The results described here indicate that other Ti plasmid sequences than solely the T-region can be transferred to plant cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
EMBO JOURNAL
EMBO J.
volume
2
issue
12
pages
2151 - 2160
Web of Science type
Article
ISSN
0261-4189
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6889675
handle
http://hdl.handle.net/1854/LU-6889675
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC555427/
date created
2015-07-29 17:21:37
date last changed
2016-12-19 15:38:56
@article{6889675,
  abstract     = {In an attempt to elucidate the transfer and integration mechanism of Agrobacterium DNA upon crown gall induction, we translocated a borderless T-DNA to different sites of the C58 Ti plasmid. As a result of the physical linkage of the T-DNA onc genes with other Ti plasmid functions, the concerned strain retained tumor-inducing capacity. However, when the borderless T-DNA is separated on an independent replicon while all other pTi functions are provided in trans, the strain can no longer induce tumors on plants. We provide evidence that the right T-DNA border region harbors one or more in cis active functions essential in the transfer and/or stabilization of the T-DNA into plant cells. The strains used in these experiments allowed us to conclude that some function(s) of the Ti plasmid can induce plant cell proliferations independently of the T-DNA transformation event. The results described here indicate that other Ti plasmid sequences than solely the T-region can be transferred to plant cells.},
  author       = {Joos, Henk and Timmerman, Benedikt and Van Montagu, Marc and Schell, Jeff},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  language     = {eng},
  number       = {12},
  pages        = {2151--2160},
  title        = {Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells},
  url          = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC555427/},
  volume       = {2},
  year         = {1983},
}

Chicago
Joos, Henk, Benedikt Timmerman, Marc Van Montagu, and Jeff Schell. 1983. “Genetic Analysis of Transfer and Stabilization of Agrobacterium DNA in Plant Cells.” Embo Journal 2 (12): 2151–2160.
APA
Joos, H., Timmerman, B., Van Montagu, M., & Schell, J. (1983). Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells. EMBO JOURNAL, 2(12), 2151–2160.
Vancouver
1.
Joos H, Timmerman B, Van Montagu M, Schell J. Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells. EMBO JOURNAL. 1983;2(12):2151–60.
MLA
Joos, Henk, Benedikt Timmerman, Marc Van Montagu, et al. “Genetic Analysis of Transfer and Stabilization of Agrobacterium DNA in Plant Cells.” EMBO JOURNAL 2.12 (1983): 2151–2160. Print.