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Protection against influenza A virus challenge with M2e-displaying filamentous Escherichia coli phages

(2015) PLOS ONE. 10(5).
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Abstract
Human influenza viruses are responsible for annual epidemics and occasional pandemics that cause severe illness and mortality in all age groups worldwide. Matrix protein 2 (M2) of influenza A virus is a tetrameric type III membrane protein that functions as a proton-selective channel. The extracellular domain of M2 (M2e) is conserved in human and avian influenza A viruses and is being pursued as a component for a universal influenza A vaccine. To develop a M2e vaccine that is economical and easy to purify, we genetically fused M2e amino acids 2-16 to the N-terminus of pVIII, the major coat protein of filamentous bacteriophage f88. We show that the resulting recombinant f88-M2e2-16 phages are replication competent and display the introduced part of M2e on the phage surface. Immunization of mice with purified f88-M2e2-16 phages in the presence of incomplete Freund's adjuvant, induced robust M2e-specific serum IgG and protected BALB/c mice against challenge with human and avian influenza A viruses. Thus, replication competent filamentous bacteriophages can be used as efficient and economical carriers to display conserved B cell epitopes of influenza A.
Keywords
MATRIX PROTEIN-2, M2 PROTEIN, IMMUNE-RESPONSES, EXTRACELLULAR DOMAIN, MONOCLONAL-ANTIBODY, MEMBRANE-PROTEIN, INFECTED-CELLS, VACCINE, IMMUNIZATION, MICE

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Citation

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MLA
Deng, Lei et al. “Protection Against Influenza A Virus Challenge with M2e-displaying Filamentous Escherichia Coli Phages.” PLOS ONE 10.5 (2015): n. pag. Print.
APA
Deng, L., Ibanez, L., VAN DEN BOSSCHE, V., Roose, K., Youssef, S. A., de Bruin, A., Fiers, W., et al. (2015). Protection against influenza A virus challenge with M2e-displaying filamentous Escherichia coli phages. PLOS ONE, 10(5).
Chicago author-date
Deng, Lei, Lorena Ibanez, VERONIQUE VAN DEN BOSSCHE, Kenny Roose, Sameh A Youssef, Alain de Bruin, Walter Fiers, and Xavier Saelens. 2015. “Protection Against Influenza A Virus Challenge with M2e-displaying Filamentous Escherichia Coli Phages.” Plos One 10 (5).
Chicago author-date (all authors)
Deng, Lei, Lorena Ibanez, VERONIQUE VAN DEN BOSSCHE, Kenny Roose, Sameh A Youssef, Alain de Bruin, Walter Fiers, and Xavier Saelens. 2015. “Protection Against Influenza A Virus Challenge with M2e-displaying Filamentous Escherichia Coli Phages.” Plos One 10 (5).
Vancouver
1.
Deng L, Ibanez L, VAN DEN BOSSCHE V, Roose K, Youssef SA, de Bruin A, et al. Protection against influenza A virus challenge with M2e-displaying filamentous Escherichia coli phages. PLOS ONE. 2015;10(5).
IEEE
[1]
L. Deng et al., “Protection against influenza A virus challenge with M2e-displaying filamentous Escherichia coli phages,” PLOS ONE, vol. 10, no. 5, 2015.
@article{6889062,
  abstract     = {Human influenza viruses are responsible for annual epidemics and occasional pandemics that cause severe illness and mortality in all age groups worldwide. Matrix protein 2 (M2) of influenza A virus is a tetrameric type III membrane protein that functions as a proton-selective channel. The extracellular domain of M2 (M2e) is conserved in human and avian influenza A viruses and is being pursued as a component for a universal influenza A vaccine. To develop a M2e vaccine that is economical and easy to purify, we genetically fused M2e amino acids 2-16 to the N-terminus of pVIII, the major coat protein of filamentous bacteriophage f88. We show that the resulting recombinant f88-M2e2-16 phages are replication competent and display the introduced part of M2e on the phage surface. Immunization of mice with purified f88-M2e2-16 phages in the presence of incomplete Freund's adjuvant, induced robust M2e-specific serum IgG and protected BALB/c mice against challenge with human and avian influenza A viruses. Thus, replication competent filamentous bacteriophages can be used as efficient and economical carriers to display conserved B cell epitopes of influenza A.},
  articleno    = {e0126650},
  author       = {Deng, Lei and Ibanez, Lorena and VAN DEN BOSSCHE, VERONIQUE and Roose, Kenny and Youssef, Sameh A and de Bruin, Alain and Fiers, Walter and Saelens, Xavier},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {MATRIX PROTEIN-2,M2 PROTEIN,IMMUNE-RESPONSES,EXTRACELLULAR DOMAIN,MONOCLONAL-ANTIBODY,MEMBRANE-PROTEIN,INFECTED-CELLS,VACCINE,IMMUNIZATION,MICE},
  language     = {eng},
  number       = {5},
  pages        = {21},
  title        = {Protection against influenza A virus challenge with M2e-displaying filamentous Escherichia coli phages},
  url          = {http://dx.doi.org/10.1371/journal.pone.0126650},
  volume       = {10},
  year         = {2015},
}

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