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Raised immunoglobulin A and circulating T follicular helper cells are linked to the development of food allergy in paediatric liver transplant patients

(2015) CLINICAL AND EXPERIMENTAL ALLERGY. 45(6). p.1060-1070
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Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
Abstract
Background: Post-transplant food allergy (LTFA) is increasingly observed after paediatric liver transplantation (LT). Although the immunopathology of LTFA remains unclear, immunoglobulin (Ig) E seems to be implicated. Objective: To study humoral and cellular immunity in paediatric LT patients in search for factors associated with LTFA, and compare with healthy controls (HC) and non-transplant food-allergic children (FA). Methods: We studied serum Ig levels in 29 LTFA, 43 non-food-allergic LT patients (LTnoFA), 21 FA patients and 36 HC. Serum-specific IgA and IgE against common food allergens in LTFA, IgA(1), IgA(2) and joining-chain-containing polymeric IgA (pIgA) were measured. Peripheral blood mononuclear cells were analysed by flow cytometry for B and T cell populations of interest. Results: Serum IgA and specific IgA were higher in LTFA compared to LTnoFA. LTFA patients had the highest proportion of circulating T follicular helper cells (cTfh). The percentage of cTfh correlated positively with serum IgA. Unique in LTFA was also the significant increase in serum markers of mucosal IgA and the decrease in the Th17 subset of CXCR5(-) CD4(+) cells compared to HC. Both LT patients exhibited a rise in IgA(+) memory B cells and plasmablasts compared to HC and FA. Conclusions: LT has an impact on humoral immunity, remarkably in those patients developing FA. The increase in serum markers of mucosal IgA, food allergen-specific IgA and cTfh cells observed in LTFA, point towards a disturbance in intestinal immune homoeostasis in this patient group.
Keywords
liver transplantation, immunoglobulin A, food allergy, children, polymeric IgA, secretory IgA, specific IgA, T follicular helper cells, regulatory T cells, SECRETORY IGA, MUCOSAL, GUT, EXPRESSION, ANTIBODIES, TOLERANCE, TRANSPORT, CHILDREN, MOUSE, FK506

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Citation

Please use this url to cite or link to this publication:

Chicago
De Bruyne, Ruth, Philippe Gevaert, Myriam Van Winckel, Natalie De Ruyck, Aurelie Minne, Delfien Bogaert, Stephanie Van Biervliet, et al. 2015. “Raised Immunoglobulin A and Circulating T Follicular Helper Cells Are Linked to the Development of Food Allergy in Paediatric Liver Transplant Patients.” Clinical and Experimental Allergy 45 (6): 1060–1070.
APA
De Bruyne, R., Gevaert, P., Van Winckel, M., De Ruyck, N., Minne, A., Bogaert, D., Van Biervliet, S., et al. (2015). Raised immunoglobulin A and circulating T follicular helper cells are linked to the development of food allergy in paediatric liver transplant patients. CLINICAL AND EXPERIMENTAL ALLERGY, 45(6), 1060–1070.
Vancouver
1.
De Bruyne R, Gevaert P, Van Winckel M, De Ruyck N, Minne A, Bogaert D, et al. Raised immunoglobulin A and circulating T follicular helper cells are linked to the development of food allergy in paediatric liver transplant patients. CLINICAL AND EXPERIMENTAL ALLERGY. 2015;45(6):1060–70.
MLA
De Bruyne, Ruth, Philippe Gevaert, Myriam Van Winckel, et al. “Raised Immunoglobulin A and Circulating T Follicular Helper Cells Are Linked to the Development of Food Allergy in Paediatric Liver Transplant Patients.” CLINICAL AND EXPERIMENTAL ALLERGY 45.6 (2015): 1060–1070. Print.
@article{6888341,
  abstract     = {Background: Post-transplant food allergy (LTFA) is increasingly observed after paediatric liver transplantation (LT). Although the immunopathology of LTFA remains unclear, immunoglobulin (Ig) E seems to be implicated.
Objective: To study humoral and cellular immunity in paediatric LT patients in search for factors associated with LTFA, and compare with healthy controls (HC) and non-transplant food-allergic children (FA).
Methods: We studied serum Ig levels in 29 LTFA, 43 non-food-allergic LT patients (LTnoFA), 21 FA patients and 36 HC. Serum-specific IgA and IgE against common food allergens in LTFA, IgA(1), IgA(2) and joining-chain-containing polymeric IgA (pIgA) were measured. Peripheral blood mononuclear cells were analysed by flow cytometry for B and T cell populations of interest.
Results: Serum IgA and specific IgA were higher in LTFA compared to LTnoFA. LTFA patients had the highest proportion of circulating T follicular helper cells (cTfh). The percentage of cTfh correlated positively with serum IgA. Unique in LTFA was also the significant increase in serum markers of mucosal IgA and the decrease in the Th17 subset of CXCR5(-) CD4(+) cells compared to HC. Both LT patients exhibited a rise in IgA(+) memory B cells and plasmablasts compared to HC and FA.
Conclusions: LT has an impact on humoral immunity, remarkably in those patients developing FA. The increase in serum markers of mucosal IgA, food allergen-specific IgA and cTfh cells observed in LTFA, point towards a disturbance in intestinal immune homoeostasis in this patient group.},
  author       = {De Bruyne, Ruth and Gevaert, Philippe and Van Winckel, Myriam and De Ruyck, Natalie and Minne, Aurelie and Bogaert, Delfien and Van Biervliet, Stephanie and Vande Velde, Saskia and Smets, Francoise and Sokal, Etienne and Gottrand, Fr{\'e}deric and Vanhelst, J{\'e}r{\'e}my and Detry, Bruno and Pilette, Charles and Lambrecht, Bart and Dullaers, Melissa},
  issn         = {0954-7894},
  journal      = {CLINICAL AND EXPERIMENTAL ALLERGY},
  language     = {eng},
  number       = {6},
  pages        = {1060--1070},
  title        = {Raised immunoglobulin A and circulating T follicular helper cells are linked to the development of food allergy in paediatric liver transplant patients},
  url          = {http://dx.doi.org/10.1111/cea.12514},
  volume       = {45},
  year         = {2015},
}

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