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In vitro induction of tetraploids in ornamental Ranunculus

(2009) EUPHYTICA. 168(1). p.33-40
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Abstract
This study investigates the capacity of the antimitotic agents colchicine, oryzalin and trifluralin for inducing polyploidisation of Ranunculus asiaticus 'Alfa' in vitro shoots. Flow cytometry was used to evaluate the optimal concentration of each antimitotic agent for polyploidisation. Trifluralin at a concentration of 2 mu M resulted in the highest percentage of polyploidisation (27.5%), followed by a colchicine treatment of 200 mu M, which induced 23.3% of polyploids. For oryzalin the highest percentage was achieved using a concentration of 1 mu M. Different exposure periods were tested and turned out to be an important factor. The maximal exposure period tested (10 weeks) resulted in a significant increase in polyploidisation by oryzalin and trifluralin. In contrast, for colchicine (100 mu M) exposure times of either 16 or 24 h did not significantly influence polyploidisation. Additionally the effect of the antimitotic agents on the viability was analysed. For colchicine no significant effect on the survival rate was observed, for trifluralin only a concentration of 10 mu M affected viability whereas for oryzalin, concentration as well as exposure period were significant parameters. Flow cytometric data were confirmed by counting chromosomes in root tip cells.
Keywords
Colchicine, Oryzalin, Polyploidisation, Ranunculaceae, Trifluralin, DINITROANILINE HERBICIDE, ORYZALIN, PLANT, ROSA, POLYPLOIDIZATION, ADONEUS, TUBULIN, TIME, DNA

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Citation

Please use this url to cite or link to this publication:

MLA
Dhooghe, Emmy et al. “In Vitro Induction of Tetraploids in Ornamental Ranunculus.” EUPHYTICA 168.1 (2009): 33–40. Print.
APA
Dhooghe, E., Denis, S., Eeckhaut, T., Reheul, D., & Van Labeke, M.-C. (2009). In vitro induction of tetraploids in ornamental Ranunculus. EUPHYTICA, 168(1), 33–40.
Chicago author-date
Dhooghe, Emmy, Sylvie Denis, Tom Eeckhaut, Dirk Reheul, and Marie-Christine Van Labeke. 2009. “In Vitro Induction of Tetraploids in Ornamental Ranunculus.” Euphytica 168 (1): 33–40.
Chicago author-date (all authors)
Dhooghe, Emmy, Sylvie Denis, Tom Eeckhaut, Dirk Reheul, and Marie-Christine Van Labeke. 2009. “In Vitro Induction of Tetraploids in Ornamental Ranunculus.” Euphytica 168 (1): 33–40.
Vancouver
1.
Dhooghe E, Denis S, Eeckhaut T, Reheul D, Van Labeke M-C. In vitro induction of tetraploids in ornamental Ranunculus. EUPHYTICA. 2009;168(1):33–40.
IEEE
[1]
E. Dhooghe, S. Denis, T. Eeckhaut, D. Reheul, and M.-C. Van Labeke, “In vitro induction of tetraploids in ornamental Ranunculus,” EUPHYTICA, vol. 168, no. 1, pp. 33–40, 2009.
@article{688081,
  abstract     = {This study investigates the capacity of the antimitotic agents colchicine, oryzalin and trifluralin for inducing polyploidisation of Ranunculus asiaticus 'Alfa' in vitro shoots. Flow cytometry was used to evaluate the optimal concentration of each antimitotic agent for polyploidisation. Trifluralin at a concentration of 2 mu M resulted in the highest percentage of polyploidisation (27.5%), followed by a colchicine treatment of 200 mu M, which induced 23.3% of polyploids. For oryzalin the highest percentage was achieved using a concentration of 1 mu M. Different exposure periods were tested and turned out to be an important factor. The maximal exposure period tested (10 weeks) resulted in a significant increase in polyploidisation by oryzalin and trifluralin. In contrast, for colchicine (100 mu M) exposure times of either 16 or 24 h did not significantly influence polyploidisation. Additionally the effect of the antimitotic agents on the viability was analysed. For colchicine no significant effect on the survival rate was observed, for trifluralin only a concentration of 10 mu M affected viability whereas for oryzalin, concentration as well as exposure period were significant parameters. Flow cytometric data were confirmed by counting chromosomes in root tip cells.},
  author       = {Dhooghe, Emmy and Denis, Sylvie and Eeckhaut, Tom and Reheul, Dirk and Van Labeke, Marie-Christine},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keywords     = {Colchicine,Oryzalin,Polyploidisation,Ranunculaceae,Trifluralin,DINITROANILINE HERBICIDE,ORYZALIN,PLANT,ROSA,POLYPLOIDIZATION,ADONEUS,TUBULIN,TIME,DNA},
  language     = {eng},
  number       = {1},
  pages        = {33--40},
  title        = {In vitro induction of tetraploids in ornamental Ranunculus},
  url          = {http://dx.doi.org/10.1007/s10681-008-9876-1},
  volume       = {168},
  year         = {2009},
}

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