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In vitro simulation of the hindgut as a tool to study the influence of phytosterol consumption on the excretion of anabolic-androgenic steroids in horses

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Abstract
Introduction: In race horses zero tolerance is held for most anabolic steroids (AAS). Nevertheless, some natural precursors, including phytosterols from grains, have been put forward as a possible explanation for the endogenous prevalence of low concentrations of steroids, with β-Bol as the most illicit candidate. Purpose: This study aimed to evaluate the use of in vitro digestions as a tool to simulate in vivo hindgut fermentation and to study the possible biotransformation from phytosterols or other androgenic precursors to forbidden AAS. Methods: To investigate the possible endogenous biotransformation, in vitro simulations of the horse hindgut were set up, using fecal inocula obtained from eight different horses. In vitro digestion samples were analyzed with a fully validated UHPLC-MS/MS method. Results: The digestive transformation of ADD could be directly linked to the detection of βBol, and the consumption of phytosterols to low levels of AED, a testosterone precursor, but no direct link between phytosterol consumption and βBol detection was observed. Significance: The results are in line with previously described in vivo results, proving that in vitro digestions are a useful tool to study the fermentation reactions that take place in the horse’s hindgut, offering new insights in the detection of, endogenous, anabolic steroids.

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Chicago
Decloedt, Anneleen, Ludovic Bailly-Chouriberry, Yves Bonnaire, and Lynn Vanhaecke. 2015. “In Vitro Simulation of the Hindgut as a Tool to Study the Influence of Phytosterol Consumption on the Excretion of Anabolic-androgenic Steroids in Horses.” In Validation and Regulatory Analysis, 3rd Saskatoon International Workshop, Abstracts.
APA
Decloedt, Anneleen, Bailly-Chouriberry, L., Bonnaire, Y., & Vanhaecke, L. (2015). In vitro simulation of the hindgut as a tool to study the influence of phytosterol consumption on the excretion of anabolic-androgenic steroids in horses. Validation and Regulatory Analysis, 3rd Saskatoon international workshop, Abstracts. Presented at the 3rd Saskatoon international workshop on Validation and Regulatory Analysis.
Vancouver
1.
Decloedt A, Bailly-Chouriberry L, Bonnaire Y, Vanhaecke L. In vitro simulation of the hindgut as a tool to study the influence of phytosterol consumption on the excretion of anabolic-androgenic steroids in horses. Validation and Regulatory Analysis, 3rd Saskatoon international workshop, Abstracts. 2015.
MLA
Decloedt, Anneleen, Ludovic Bailly-Chouriberry, Yves Bonnaire, et al. “In Vitro Simulation of the Hindgut as a Tool to Study the Influence of Phytosterol Consumption on the Excretion of Anabolic-androgenic Steroids in Horses.” Validation and Regulatory Analysis, 3rd Saskatoon International Workshop, Abstracts. 2015. Print.
@inproceedings{6870178,
  abstract     = {Introduction: In race horses zero tolerance is held for most anabolic steroids (AAS). Nevertheless, some natural precursors, including phytosterols from grains, have been put forward as a possible explanation for the endogenous prevalence of low concentrations of steroids, with \ensuremath{\beta}-Bol as the most illicit candidate. 
Purpose: This study aimed to evaluate the use of in vitro digestions as a tool to simulate in vivo hindgut fermentation and to study the possible biotransformation from phytosterols or other androgenic precursors to forbidden AAS.
Methods: To investigate the possible endogenous biotransformation, in vitro simulations of the horse hindgut were set up, using fecal inocula obtained from eight different horses. In vitro digestion samples were analyzed with a fully validated UHPLC-MS/MS method.
Results: The digestive transformation of ADD could be directly linked to the detection of \ensuremath{\beta}Bol, and the consumption of phytosterols to low levels of AED, a testosterone precursor, but no direct link between phytosterol consumption and \ensuremath{\beta}Bol detection was observed.
Significance: The results are in line with previously described in vivo results, proving that in vitro digestions are a useful tool to study the fermentation reactions that take place in the horse{\textquoteright}s hindgut, offering new insights in the detection of, endogenous, anabolic steroids.},
  author       = {Decloedt, Anneleen and Bailly-Chouriberry, Ludovic and Bonnaire, Yves and Vanhaecke, Lynn},
  booktitle    = {Validation and Regulatory Analysis, 3rd Saskatoon international workshop, Abstracts},
  language     = {eng},
  location     = {Calgary, AB, Canada},
  title        = {In vitro simulation of the hindgut as a tool to study the influence of phytosterol consumption on the excretion of anabolic-androgenic steroids in horses},
  year         = {2015},
}