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Porting forensic DNA analysis to deep sequencing

(2015)
Author
Promoter
(UGent) , (UGent) and (UGent)
Organization
Project
Bioinformatics: from nucleotids to networks (N2N)
Abstract
Forensic DNA profiles of short tandem repeat (STR) loci are currently obtained using PCR followed by capillary electrophoresis (CE). Massively parallel sequencing (MPS) technologies do not rely on size separation and thus relieve the limitations on locus multiplexy. Deep sequencing with MPS creates possibilities within forensics for analyzing degraded samples and mixed samples. Furthermore, in the same analysis single nucleotide polymorphism (SNP) markers can be included, which can generate phenotypic or ancestry leads for forensic investigators. Data analysis of raw sequencer reads, resulting in a reliable and usable forensic human identification report is still in early development. The aim of the doctoral research was to develop a program for forensic DNA data analysis. The main results are the data analysis framework MyFLq (My Forensic Loci queries) and nomenclature service FLAD (Forensic Loci Allele Database). MyFLq and FLAD can be used together in a forensic workflow that has backward compatibility with CE. To my knowledge, this is the first open-source and complete solution for forensic MPS raw data analysis.
Keywords
sequencing, forensics, SNP, STR, MPS, NGS

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Citation

Please use this url to cite or link to this publication:

Chicago
Van Neste, Christophe. 2015. “Porting Forensic DNA Analysis to Deep Sequencing”. Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences.
APA
Van Neste, C. (2015). Porting forensic DNA analysis to deep sequencing. Ghent University. Faculty of Pharmaceutical Sciences, Ghent, Belgium.
Vancouver
1.
Van Neste C. Porting forensic DNA analysis to deep sequencing. [Ghent, Belgium]: Ghent University. Faculty of Pharmaceutical Sciences; 2015.
MLA
Van Neste, Christophe. “Porting Forensic DNA Analysis to Deep Sequencing.” 2015 : n. pag. Print.
@phdthesis{6868627,
  abstract     = {Forensic DNA profiles of short tandem repeat (STR) loci are currently obtained using PCR followed by capillary electrophoresis (CE). Massively parallel sequencing (MPS) technologies do not rely on size separation and thus relieve the limitations on locus multiplexy. Deep sequencing with MPS creates possibilities within forensics for analyzing degraded samples and mixed samples. Furthermore, in the same analysis single nucleotide polymorphism (SNP) markers can be included, which can generate phenotypic or ancestry leads for forensic investigators.
Data analysis of raw sequencer reads, resulting in a reliable and usable forensic human identification report is still in early development. The aim of the doctoral research was to develop a program for forensic DNA data analysis. The main results are the data analysis framework MyFLq (My Forensic Loci queries) and nomenclature service FLAD (Forensic Loci Allele Database). MyFLq and FLAD can be used together in a forensic workflow that has backward compatibility with CE. To my knowledge, this is the first open-source and complete solution for forensic MPS raw data analysis.},
  author       = {Van Neste, Christophe},
  isbn         = {9789059898042},
  keyword      = {sequencing,forensics,SNP,STR,MPS,NGS},
  language     = {eng},
  pages        = {XXVII, 162},
  publisher    = {Ghent University. Faculty of Pharmaceutical Sciences},
  school       = {Ghent University},
  title        = {Porting forensic DNA analysis to deep sequencing},
  year         = {2015},
}