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The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity

Chenjing Shang, Qiushi Chen, Anne Dell, Stuart M Haslam, Winnok De Vos UGent and Els Van Damme UGent (2015) PLOS ONE. 10(7).
abstract
Although the protein translation inhibition activity of ribosome inactivating proteins (RIPs) is well documented, little is known about the contribution of the lectin chain to the biological activity of these proteins. In this study, we compared the in vitro and intracellular activity of several S. nigra (elderberry) RIPs and non-RIP lectins. Our data demonstrate that RIPs from elderberry are much more toxic to HeLa cells than to primary fibroblasts. Differences in the cytotoxicity between the elderberry proteins correlated with differences in glycan specificity of their lectin domain, cellular uptake efficiency and intracellular destination. Despite the fact that the bulk of the RIPs accumulated in the lysosomes and partly in the Golgi apparatus, we could demonstrate effective inhibition of protein synthesis in cellula. As we also observed cytotoxicity for non-RIP lectins, it is clear that the lectin chain triggers additional pathways heralding cell death. Our data suggest that one of these pathways involves the induction of autophagy.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BINDING, TOXIN, APOPTOSIS, LECTIN, RICIN, HELA-CELLS, MOLECULAR-CLONING, GLYCOSIDASE ACTIVITY, COLOCALIZATION ANALYSIS, SAMBUCUS-NIGRA
journal title
PLOS ONE
PLoS One
volume
10
issue
7
article number
e0132389
pages
23 pages
Web of Science type
Article
Web of Science id
000358157600255
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.057 (2015)
JCR rank
11/63 (2015)
JCR quartile
1 (2015)
ISSN
1932-6203
DOI
10.1371/journal.pone.0132389
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
6867054
handle
http://hdl.handle.net/1854/LU-6867054
date created
2015-07-07 12:32:28
date last changed
2016-12-21 15:42:37
@article{6867054,
  abstract     = {Although the protein translation inhibition activity of ribosome inactivating proteins (RIPs) is well documented, little is known about the contribution of the lectin chain to the biological activity of these proteins. In this study, we compared the in vitro and intracellular activity of several S. nigra (elderberry) RIPs and non-RIP lectins. Our data demonstrate that RIPs from elderberry are much more toxic to HeLa cells than to primary fibroblasts. Differences in the cytotoxicity between the elderberry proteins correlated with differences in glycan specificity of their lectin domain, cellular uptake efficiency and intracellular destination. Despite the fact that the bulk of the RIPs accumulated in the lysosomes and partly in the Golgi apparatus, we could demonstrate effective inhibition of protein synthesis in cellula. As we also observed cytotoxicity for non-RIP lectins, it is clear that the lectin chain triggers additional pathways heralding cell death. Our data suggest that one of these pathways involves the induction of autophagy.},
  articleno    = {e0132389},
  author       = {Shang, Chenjing and Chen, Qiushi and Dell, Anne and Haslam, Stuart M and De Vos, Winnok and Van Damme, Els},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {BINDING,TOXIN,APOPTOSIS,LECTIN,RICIN,HELA-CELLS,MOLECULAR-CLONING,GLYCOSIDASE ACTIVITY,COLOCALIZATION ANALYSIS,SAMBUCUS-NIGRA},
  language     = {eng},
  number       = {7},
  pages        = {23},
  title        = {The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity},
  url          = {http://dx.doi.org/10.1371/journal.pone.0132389},
  volume       = {10},
  year         = {2015},
}

Chicago
Shang, Chenjing, Qiushi Chen, Anne Dell, Stuart M Haslam, Winnok De Vos, and Els Van Damme. 2015. “The Cytotoxicity of Elderberry Ribosome-inactivating Proteins Is Not Solely Determined by Their Protein Translation Inhibition Activity.” Plos One 10 (7).
APA
Shang, C., Chen, Q., Dell, A., Haslam, S. M., De Vos, W., & Van Damme, E. (2015). The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity. PLOS ONE, 10(7).
Vancouver
1.
Shang C, Chen Q, Dell A, Haslam SM, De Vos W, Van Damme E. The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity. PLOS ONE. 2015;10(7).
MLA
Shang, Chenjing, Qiushi Chen, Anne Dell, et al. “The Cytotoxicity of Elderberry Ribosome-inactivating Proteins Is Not Solely Determined by Their Protein Translation Inhibition Activity.” PLOS ONE 10.7 (2015): n. pag. Print.