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The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity

(2015) PLOS ONE. 10(7).
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Abstract
Although the protein translation inhibition activity of ribosome inactivating proteins (RIPs) is well documented, little is known about the contribution of the lectin chain to the biological activity of these proteins. In this study, we compared the in vitro and intracellular activity of several S. nigra (elderberry) RIPs and non-RIP lectins. Our data demonstrate that RIPs from elderberry are much more toxic to HeLa cells than to primary fibroblasts. Differences in the cytotoxicity between the elderberry proteins correlated with differences in glycan specificity of their lectin domain, cellular uptake efficiency and intracellular destination. Despite the fact that the bulk of the RIPs accumulated in the lysosomes and partly in the Golgi apparatus, we could demonstrate effective inhibition of protein synthesis in cellula. As we also observed cytotoxicity for non-RIP lectins, it is clear that the lectin chain triggers additional pathways heralding cell death. Our data suggest that one of these pathways involves the induction of autophagy.
Keywords
BINDING, TOXIN, APOPTOSIS, LECTIN, RICIN, HELA-CELLS, MOLECULAR-CLONING, GLYCOSIDASE ACTIVITY, COLOCALIZATION ANALYSIS, SAMBUCUS-NIGRA

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Citation

Please use this url to cite or link to this publication:

MLA
Shang, Chenjing et al. “The Cytotoxicity of Elderberry Ribosome-inactivating Proteins Is Not Solely Determined by Their Protein Translation Inhibition Activity.” PLOS ONE 10.7 (2015): n. pag. Print.
APA
Shang, C., Chen, Q., Dell, A., Haslam, S. M., De Vos, W., & Van Damme, E. (2015). The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity. PLOS ONE, 10(7).
Chicago author-date
Shang, Chenjing, Qiushi Chen, Anne Dell, Stuart M Haslam, Winnok De Vos, and Els Van Damme. 2015. “The Cytotoxicity of Elderberry Ribosome-inactivating Proteins Is Not Solely Determined by Their Protein Translation Inhibition Activity.” Plos One 10 (7).
Chicago author-date (all authors)
Shang, Chenjing, Qiushi Chen, Anne Dell, Stuart M Haslam, Winnok De Vos, and Els Van Damme. 2015. “The Cytotoxicity of Elderberry Ribosome-inactivating Proteins Is Not Solely Determined by Their Protein Translation Inhibition Activity.” Plos One 10 (7).
Vancouver
1.
Shang C, Chen Q, Dell A, Haslam SM, De Vos W, Van Damme E. The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity. PLOS ONE. 2015;10(7).
IEEE
[1]
C. Shang, Q. Chen, A. Dell, S. M. Haslam, W. De Vos, and E. Van Damme, “The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity,” PLOS ONE, vol. 10, no. 7, 2015.
@article{6867054,
  abstract     = {Although the protein translation inhibition activity of ribosome inactivating proteins (RIPs) is well documented, little is known about the contribution of the lectin chain to the biological activity of these proteins. In this study, we compared the in vitro and intracellular activity of several S. nigra (elderberry) RIPs and non-RIP lectins. Our data demonstrate that RIPs from elderberry are much more toxic to HeLa cells than to primary fibroblasts. Differences in the cytotoxicity between the elderberry proteins correlated with differences in glycan specificity of their lectin domain, cellular uptake efficiency and intracellular destination. Despite the fact that the bulk of the RIPs accumulated in the lysosomes and partly in the Golgi apparatus, we could demonstrate effective inhibition of protein synthesis in cellula. As we also observed cytotoxicity for non-RIP lectins, it is clear that the lectin chain triggers additional pathways heralding cell death. Our data suggest that one of these pathways involves the induction of autophagy.},
  articleno    = {e0132389},
  author       = {Shang, Chenjing and Chen, Qiushi and Dell, Anne and Haslam, Stuart M and De Vos, Winnok and Van Damme, Els},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {BINDING,TOXIN,APOPTOSIS,LECTIN,RICIN,HELA-CELLS,MOLECULAR-CLONING,GLYCOSIDASE ACTIVITY,COLOCALIZATION ANALYSIS,SAMBUCUS-NIGRA},
  language     = {eng},
  number       = {7},
  pages        = {23},
  title        = {The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity},
  url          = {http://dx.doi.org/10.1371/journal.pone.0132389},
  volume       = {10},
  year         = {2015},
}

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