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ALTERNATIVE OXIDASE1a modulates the oxidative challenge during moderate Cd exposure in Arabidopsis thaliana leaves

Els Keunen, Kerim Schellingen, Dominique Van Der Straeten UGent, Tony Remans, Jan Colpaert, Jaco Vangronsveld and Ann Cuypers (2015) JOURNAL OF EXPERIMENTAL BOTANY. 66(10). p.2967-2977
abstract
This study aims to unravel the functional significance of alternative oxidase1a (AOX1a) induction in Arabidopsis thaliana leaves exposed to cadmium (Cd) by comparing wild-type (WT) plants and aox1a knockout mutants. In the absence of AOX1a, differences in stress-responsive transcript and glutathione levels suggest an increased oxidative challenge during moderate (5 mu M) and prolonged (72 h) Cd exposure. Nevertheless, aox1a knockout leaves showed lower hydrogen peroxide (H2O2) accumulation as compared to the WT due to both acute (24 h) and prolonged (72 h) exposure to 5 mu M Cd, but not to 10 mu M Cd. Taken together, we propose a working model where AOX1a acts early in the response to Cd and activates or maintains a mitochondrial signalling pathway impacting on cellular antioxidative defence at the post-transcriptional level. This fine-tuning pathway is suggested to function during moderate (5 mu M) Cd exposure while being overwhelmed during more severe (10 mu M) Cd stress. Within this framework, ethylene is required - either directly or indirectly via NADPH oxidase isoform C - to fully induce AOX1 expression. In addition, reciprocal crosstalk between these components was demonstrated in leaves of A. thaliana plants exposed to Cd.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INDUCTION, METABOLISM, STRESS, ETHYLENE, CADMIUM, NADPH OXIDASE, TOBACCO PLANTS, RESPIRATORY PATHWAY, NITRIC-OXIDE, GENE-EXPRESSION, oxidative challenge, ethylene, cadmium (Cd), Arabidopsis thaliana, alternative respiration, Alternative oxidase
journal title
JOURNAL OF EXPERIMENTAL BOTANY
J. Exp. Bot.
volume
66
issue
10
pages
2967 - 2977
Web of Science type
Article
Web of Science id
000356223900014
JCR category
PLANT SCIENCES
JCR impact factor
5.677 (2015)
JCR rank
12/209 (2015)
JCR quartile
1 (2015)
ISSN
0022-0957
DOI
10.1093/jxb/erv035
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6867019
handle
http://hdl.handle.net/1854/LU-6867019
date created
2015-07-07 12:10:09
date last changed
2016-12-19 15:39:09
@article{6867019,
  abstract     = {This study aims to unravel the functional significance of alternative oxidase1a (AOX1a) induction in Arabidopsis thaliana leaves exposed to cadmium (Cd) by comparing wild-type (WT) plants and aox1a knockout mutants. In the absence of AOX1a, differences in stress-responsive transcript and glutathione levels suggest an increased oxidative challenge during moderate (5 mu M) and prolonged (72 h) Cd exposure. Nevertheless, aox1a knockout leaves showed lower hydrogen peroxide (H2O2) accumulation as compared to the WT due to both acute (24 h) and prolonged (72 h) exposure to 5 mu M Cd, but not to 10 mu M Cd. Taken together, we propose a working model where AOX1a acts early in the response to Cd and activates or maintains a mitochondrial signalling pathway impacting on cellular antioxidative defence at the post-transcriptional level. This fine-tuning pathway is suggested to function during moderate (5 mu M) Cd exposure while being overwhelmed during more severe (10 mu M) Cd stress. Within this framework, ethylene is required - either directly or indirectly via NADPH oxidase isoform C - to fully induce AOX1 expression. In addition, reciprocal crosstalk between these components was demonstrated in leaves of A. thaliana plants exposed to Cd.},
  author       = {Keunen, Els and Schellingen, Kerim and Van Der Straeten, Dominique and Remans, Tony and Colpaert, Jan and Vangronsveld, Jaco and Cuypers, Ann},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {INDUCTION,METABOLISM,STRESS,ETHYLENE,CADMIUM,NADPH OXIDASE,TOBACCO PLANTS,RESPIRATORY PATHWAY,NITRIC-OXIDE,GENE-EXPRESSION,oxidative challenge,ethylene,cadmium (Cd),Arabidopsis thaliana,alternative respiration,Alternative oxidase},
  language     = {eng},
  number       = {10},
  pages        = {2967--2977},
  title        = {ALTERNATIVE OXIDASE1a modulates the oxidative challenge during moderate Cd exposure in Arabidopsis thaliana leaves},
  url          = {http://dx.doi.org/10.1093/jxb/erv035},
  volume       = {66},
  year         = {2015},
}

Chicago
Keunen, Els, Kerim Schellingen, Dominique Van Der Straeten, Tony Remans, Jan Colpaert, Jaco Vangronsveld, and Ann Cuypers. 2015. “ALTERNATIVE OXIDASE1a Modulates the Oxidative Challenge During Moderate Cd Exposure in Arabidopsis Thaliana Leaves.” Journal of Experimental Botany 66 (10): 2967–2977.
APA
Keunen, E., Schellingen, K., Van Der Straeten, D., Remans, T., Colpaert, J., Vangronsveld, J., & Cuypers, A. (2015). ALTERNATIVE OXIDASE1a modulates the oxidative challenge during moderate Cd exposure in Arabidopsis thaliana leaves. JOURNAL OF EXPERIMENTAL BOTANY, 66(10), 2967–2977.
Vancouver
1.
Keunen E, Schellingen K, Van Der Straeten D, Remans T, Colpaert J, Vangronsveld J, et al. ALTERNATIVE OXIDASE1a modulates the oxidative challenge during moderate Cd exposure in Arabidopsis thaliana leaves. JOURNAL OF EXPERIMENTAL BOTANY. 2015;66(10):2967–77.
MLA
Keunen, Els, Kerim Schellingen, Dominique Van Der Straeten, et al. “ALTERNATIVE OXIDASE1a Modulates the Oxidative Challenge During Moderate Cd Exposure in Arabidopsis Thaliana Leaves.” JOURNAL OF EXPERIMENTAL BOTANY 66.10 (2015): 2967–2977. Print.