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New methods to study anaerobic microbial metabolism

Jan Arends UGent, Antonin Prévoteau UGent, Annelies Geirnaert, Levi Buysschaert, Tom Van de Wiele UGent and Korneel Rabaey UGent (2015) Anaerobic Microbiology, 9th International symposium, Abstracts. p.25-25
abstract
Microbial electrosynthesis (MES) is an emerging technology aimed at converting excess electrical energy into storable biofuels or chemical precursors. The technology relies on the anaerobic microbial conversion of CO2 or organics into desired products, like CO2 to acetate by homoacetogens. The objective of this work is to develop techniques for screening microorganisms and understand how desired products will inhibit or stimulate growth of the microorganism and/or the conversion of the substrate into the desired product. The developed techniques are not limited to MES but can be applied to other anaerobic microbial conversions such as in anaerobic digestion or in the colon. Model organisms used in this work are Acetobacterium woodii and Clostridium ljungdahlii for MES. Faecalibacterium prausnitzii was uses as the colon model. To assess inhibition by products under growing conditions, continuous anaerobic incubations were performed using 96 well plates in microplate readers. Substrate turnover under non-growing conditions was assessed by using riboflavin (RF) reduction as an electrochemical probe. RF reduction was measured with a rotating disk electrode. The same experiments, under non-growing conditions, were also performed spectophotometrically. The obtained RF reduction rates could be recalculated into a substrate conversion rate per microorganism for both the RDE and spectrophotometric data. The developed methods were useful in determining product inhibitions under growing and non-growing conditions. For example: (i) anaerobic growth of the organisms was highly similar in hungate tubes compared to well plate incubations and (ii) riboflavin turnover by F. prausnitzii as determined electrochemically as well as spectrophotometrically yielded similar rates.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
Anaerobic Microbiology, 9th International symposium, Abstracts
editor
Lijana Fanedl and Gorazd Avguštin
pages
25 - 25
publisher
University of Ljubljana. Biotechnical Faculty. Animal Science department
conference name
9th International symposium on Anaerobic Microbiology (ISAM 2015)
conference location
Portorož, Slovenia
conference start
2015-06-25
conference end
2015-06-27
ISBN
9789616204712
project
Electrotalk
language
English
UGent publication?
yes
classification
C3
id
6863806
handle
http://hdl.handle.net/1854/LU-6863806
date created
2015-07-06 10:08:45
date last changed
2016-12-19 15:36:32
@inproceedings{6863806,
  abstract     = {Microbial electrosynthesis (MES) is an emerging technology aimed at converting excess electrical energy into storable biofuels or chemical precursors. The technology relies on the anaerobic microbial conversion of CO2 or organics into desired products, like CO2 to acetate by homoacetogens.
The objective of this work is to develop techniques for screening microorganisms and understand how desired products will inhibit or stimulate growth of the microorganism and/or the conversion of the substrate into the desired product. The developed techniques are not limited to MES but can be applied to other anaerobic microbial conversions such as in anaerobic digestion or in the colon. Model organisms used in this work are Acetobacterium woodii and Clostridium ljungdahlii for MES. Faecalibacterium prausnitzii was uses as the colon model.
To assess inhibition by products under growing conditions, continuous anaerobic incubations were performed using 96 well plates in microplate readers. 
Substrate turnover under non-growing conditions was assessed by using riboflavin (RF) reduction as an electrochemical probe. RF reduction was measured with a rotating disk electrode. The same experiments, under non-growing conditions, were also performed spectophotometrically. The obtained RF reduction rates could be recalculated into a substrate conversion rate per microorganism for both the RDE and spectrophotometric data.
The developed methods were useful in determining product inhibitions under growing and non-growing conditions. For example: (i) anaerobic growth of the organisms was highly similar in hungate tubes compared to well plate incubations and (ii) riboflavin turnover by F. prausnitzii as determined electrochemically as well as spectrophotometrically yielded similar rates.},
  author       = {Arends, Jan and Pr{\'e}voteau, Antonin and Geirnaert, Annelies and Buysschaert, Levi and Van de Wiele, Tom and Rabaey, Korneel},
  booktitle    = {Anaerobic Microbiology, 9th International symposium, Abstracts},
  editor       = {Fanedl, Lijana and Avgu\v{s}tin, Gorazd},
  isbn         = {9789616204712},
  language     = {eng},
  location     = {Portoro\v{z}, Slovenia},
  pages        = {25--25},
  publisher    = {University of Ljubljana. Biotechnical Faculty. Animal Science department},
  title        = {New methods to study anaerobic microbial metabolism},
  year         = {2015},
}

Chicago
Arends, Jan, Antonin Prévoteau, Annelies Geirnaert, Levi Buysschaert, Tom Van de Wiele, and Korneel Rabaey. 2015. “New Methods to Study Anaerobic Microbial Metabolism.” In Anaerobic Microbiology, 9th International Symposium, Abstracts, ed. Lijana Fanedl and Gorazd Avguštin, 25–25. University of Ljubljana. Biotechnical Faculty. Animal Science department.
APA
Arends, Jan, Prévoteau, A., Geirnaert, A., Buysschaert, L., Van de Wiele, T., & Rabaey, K. (2015). New methods to study anaerobic microbial metabolism. In L. Fanedl & G. Avguštin (Eds.), Anaerobic Microbiology, 9th International symposium, Abstracts (pp. 25–25). Presented at the 9th International symposium on Anaerobic Microbiology (ISAM 2015), University of Ljubljana. Biotechnical Faculty. Animal Science department.
Vancouver
1.
Arends J, Prévoteau A, Geirnaert A, Buysschaert L, Van de Wiele T, Rabaey K. New methods to study anaerobic microbial metabolism. In: Fanedl L, Avguštin G, editors. Anaerobic Microbiology, 9th International symposium, Abstracts. University of Ljubljana. Biotechnical Faculty. Animal Science department; 2015. p. 25–25.
MLA
Arends, Jan, Antonin Prévoteau, Annelies Geirnaert, et al. “New Methods to Study Anaerobic Microbial Metabolism.” Anaerobic Microbiology, 9th International Symposium, Abstracts. Ed. Lijana Fanedl & Gorazd Avguštin. University of Ljubljana. Biotechnical Faculty. Animal Science department, 2015. 25–25. Print.