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Exploring the potential of venom from Nasonia vitripennis as therapeutic agent with high-throughput screening tools

Ellen Danneels (UGent) , Ellen Formesyn (UGent) and Dirk de Graaf (UGent)
(2015) TOXINS. 7(6). p.2051-2070
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Abstract
The venom from the ectoparasitoid wasp Nasonia vitripennis (Hymenoptera: Pteromalidae) contains at least 80 different proteins and possibly even more peptides or other small chemical compounds, demonstrating its appealing therapeutic application. To better understand the dynamics of the venom in mammalian cells, two high-throughput screening tools were performed. The venom induced pathways related to an early stress response and activated reporters that suggest the involvement of steroids. Whether these steroids reside from the venom itself or show an induced release/production caused by the venom, still remains unsolved. The proinflammatory cytokine IL-1β was found to be down-regulated after venom and LPS co-treatment, confirming the anti-inflammatory action of N. vitripennis venom. When analyzing the expression levels of the NF-κB target genes, potentially not only the canonical but also the alternative NF-κB pathway can be affected, possibly explaining some counterintuitive results. It is proposed that next to an NF-κB binding site, the promoter of the genes tested by the PCR array may also contain binding sites for other transcription factors, resulting in a complex puzzle to connect the induced target gene with its respective transcription factor. Interestingly, Nasonia venom altered the expression of some drug targets, presenting the venom with an exciting therapeutical potential.
Keywords
PCR array, NF-κB, reporter array, therapeutic, Nasonia, venom, NF-KAPPA-B, GENE-EXPRESSION, TRANSCRIPTION FACTORS, NATURAL-PRODUCTS, TARGET GENES, CANCER CELLS, ACTIVATION, APOPTOSIS, WASP, STRESS

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Chicago
Danneels, Ellen, Ellen Formesyn, and Dirk de Graaf. 2015. “Exploring the Potential of Venom from Nasonia Vitripennis as Therapeutic Agent with High-throughput Screening Tools.” Toxins 7 (6): 2051–2070.
APA
Danneels, E., Formesyn, E., & de Graaf, D. (2015). Exploring the potential of venom from Nasonia vitripennis as therapeutic agent with high-throughput screening tools. TOXINS, 7(6), 2051–2070.
Vancouver
1.
Danneels E, Formesyn E, de Graaf D. Exploring the potential of venom from Nasonia vitripennis as therapeutic agent with high-throughput screening tools. TOXINS. 2015;7(6):2051–70.
MLA
Danneels, Ellen, Ellen Formesyn, and Dirk de Graaf. “Exploring the Potential of Venom from Nasonia Vitripennis as Therapeutic Agent with High-throughput Screening Tools.” TOXINS 7.6 (2015): 2051–2070. Print.
@article{6862838,
  abstract     = {The venom from the ectoparasitoid wasp Nasonia vitripennis (Hymenoptera: Pteromalidae) contains at least 80 different proteins and possibly even more peptides or other small chemical compounds, demonstrating its appealing therapeutic application. To better understand the dynamics of the venom in mammalian cells, two high-throughput screening tools were performed. The venom induced pathways related to an early stress response and activated reporters that suggest the involvement of steroids. Whether these steroids reside from the venom itself or show an induced release/production caused by the venom, still remains unsolved. The proinflammatory cytokine IL-1β was found to be down-regulated after venom and LPS co-treatment, confirming the anti-inflammatory action of N. vitripennis venom. When analyzing the expression levels of the NF-κB target genes, potentially not only the canonical but also the alternative NF-κB pathway can be affected, possibly explaining some counterintuitive results. It is proposed that next to an NF-κB binding site, the promoter of the genes tested by the PCR array may also contain binding sites for other transcription factors, resulting in a complex puzzle to connect the induced target gene with its respective transcription factor. Interestingly, Nasonia venom altered the expression of some drug targets, presenting the venom with an exciting therapeutical potential.},
  author       = {Danneels, Ellen and Formesyn, Ellen and de Graaf, Dirk},
  issn         = {2072-6651},
  journal      = {TOXINS},
  keywords     = {PCR array,NF-κB,reporter array,therapeutic,Nasonia,venom,NF-KAPPA-B,GENE-EXPRESSION,TRANSCRIPTION FACTORS,NATURAL-PRODUCTS,TARGET GENES,CANCER CELLS,ACTIVATION,APOPTOSIS,WASP,STRESS},
  language     = {eng},
  number       = {6},
  pages        = {2051--2070},
  title        = {Exploring the potential of venom from Nasonia vitripennis as therapeutic agent with high-throughput screening tools},
  url          = {http://dx.doi.org/10.3390/toxins7062051},
  volume       = {7},
  year         = {2015},
}

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