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Hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism : case study of Faecalibacterium prausnitzii

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  • Electrotalk
Abstract
Monitoring in vitro the metabolic activity of microorganisms aids bioprocesses and enables better understanding of microbial metabolism. Redox mediators can be used for this purpose via different electrochemical techniques that are either complex or only provide non-continuous data. Hydrodynamic chronoamperometry using a rotating disc electrode (RDE) can alleviate these issues but was seldom used and is poorly characterized. The kinetics of Faecalibacterium prausnitzii A2-165, a beneficial gut microbe, were determined using a RDE with riboflavin as redox probe. This butyrate producer anaerobically ferments glucose and reduces riboflavin whose continuous monitoring on a RDE provided highly accurate kinetic measurements of its metabolism, even at low cell densities. The metabolic reaction rate increased linearly over a broad range of cell concentrations (9 x 10(4) to 5 x 10(7) cells. mL(-1)). Apparent Michaelis-Menten kinetics was observed with respect to riboflavin (K-M = 6 mu M; k(cat) = 5.3x10(5) s(-1), at 37 degrees C) and glucose (K-M = 6 mu M; k(cat) = 2.4 x 10(5) s(-1)). The short temporal resolution allows continuous monitoring of fast cellular events such as kinetics inhibition with butyrate. Furthermore, we detected for the first time riboflavin reduction by another potential probiotic, Butyricicoccus pullicaecorum. The ability of the RDE for fast, accurate, simple and continuous measurements makes it an ad hoc tool for assessing bioprocesses at high resolution.
Keywords
BIOFUEL CELLS, GROWTH, REDOX ACTIVITY, HYDROGENASE REACTION, AMPEROMETRIC DETECTION, FERRICYANIDE REDUCTION, ELECTRON-ACCEPTOR, ESCHERICHIA-COLI, BIOCHEMICAL OXYGEN-DEMAND, DESULFOVIBRIO-VULGARIS CELLS

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Citation

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MLA
Prévoteau, Antonin et al. “Hydrodynamic Chronoamperometry for Probing Kinetics of Anaerobic Microbial Metabolism : Case Study of Faecalibacterium Prausnitzii.” SCIENTIFIC REPORTS 5 (2015): n. pag. Print.
APA
Prévoteau, A., Geirnaert, A., Arends, J., Lannebère, S., Van de Wiele, T., & Rabaey, K. (2015). Hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism : case study of Faecalibacterium prausnitzii. SCIENTIFIC REPORTS, 5.
Chicago author-date
Prévoteau, Antonin, Annelies Geirnaert, Jan Arends, Sylvain Lannebère, Tom Van de Wiele, and Korneel Rabaey. 2015. “Hydrodynamic Chronoamperometry for Probing Kinetics of Anaerobic Microbial Metabolism : Case Study of Faecalibacterium Prausnitzii.” Scientific Reports 5.
Chicago author-date (all authors)
Prévoteau, Antonin, Annelies Geirnaert, Jan Arends, Sylvain Lannebère, Tom Van de Wiele, and Korneel Rabaey. 2015. “Hydrodynamic Chronoamperometry for Probing Kinetics of Anaerobic Microbial Metabolism : Case Study of Faecalibacterium Prausnitzii.” Scientific Reports 5.
Vancouver
1.
Prévoteau A, Geirnaert A, Arends J, Lannebère S, Van de Wiele T, Rabaey K. Hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism : case study of Faecalibacterium prausnitzii. SCIENTIFIC REPORTS. 2015;5.
IEEE
[1]
A. Prévoteau, A. Geirnaert, J. Arends, S. Lannebère, T. Van de Wiele, and K. Rabaey, “Hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism : case study of Faecalibacterium prausnitzii,” SCIENTIFIC REPORTS, vol. 5, 2015.
@article{6858908,
  abstract     = {Monitoring in vitro the metabolic activity of microorganisms aids bioprocesses and enables better understanding of microbial metabolism. Redox mediators can be used for this purpose via different electrochemical techniques that are either complex or only provide non-continuous data. Hydrodynamic chronoamperometry using a rotating disc electrode (RDE) can alleviate these issues but was seldom used and is poorly characterized. The kinetics of Faecalibacterium prausnitzii A2-165, a beneficial gut microbe, were determined using a RDE with riboflavin as redox probe. This butyrate producer anaerobically ferments glucose and reduces riboflavin whose continuous monitoring on a RDE provided highly accurate kinetic measurements of its metabolism, even at low cell densities. The metabolic reaction rate increased linearly over a broad range of cell concentrations (9 x 10(4) to 5 x 10(7) cells. mL(-1)). Apparent Michaelis-Menten kinetics was observed with respect to riboflavin (K-M = 6 mu M; k(cat) = 5.3x10(5) s(-1), at 37 degrees C) and glucose (K-M = 6 mu M; k(cat) = 2.4 x 10(5) s(-1)). The short temporal resolution allows continuous monitoring of fast cellular events such as kinetics inhibition with butyrate. Furthermore, we detected for the first time riboflavin reduction by another potential probiotic, Butyricicoccus pullicaecorum. The ability of the RDE for fast, accurate, simple and continuous measurements makes it an ad hoc tool for assessing bioprocesses at high resolution.},
  articleno    = {11484},
  author       = {Prévoteau, Antonin and Geirnaert, Annelies and Arends, Jan and Lannebère, Sylvain and Van de Wiele, Tom and Rabaey, Korneel},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {BIOFUEL CELLS,GROWTH,REDOX ACTIVITY,HYDROGENASE REACTION,AMPEROMETRIC DETECTION,FERRICYANIDE REDUCTION,ELECTRON-ACCEPTOR,ESCHERICHIA-COLI,BIOCHEMICAL OXYGEN-DEMAND,DESULFOVIBRIO-VULGARIS CELLS},
  language     = {eng},
  pages        = {13},
  title        = {Hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism : case study of Faecalibacterium prausnitzii},
  url          = {http://dx.doi.org/10.1038/srep11484},
  volume       = {5},
  year         = {2015},
}

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