Advanced search
1 file | 1.22 MB Add to list

On the isolation of TI-plasmid from Agrobacterium tumefaciens

(1976) NUCLEIC ACIDS RESEARCH. 3(2). p.449-463
Author
Organization
Abstract
An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA, followed by Sarkosyl, after growth in the presence of carbenicillin. We also present a procedure for the isolation of the TI-plasmid on a large scale, that might be used for the mass isolation of other large plasmids which like the TI-plasmid, can not be cleared with earlier described procedures. The purity of the plasmid preparations was determined with DNA renaturation kinetics, which method has the advantage that the plasmid need not to be in the supercoiled or open circular form.

Downloads

  • Ledeboer et al. 1976 NucleicAcidsRes3 449.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.22 MB

Citation

Please use this url to cite or link to this publication:

MLA
Ledeboer, Adrianus M et al. “On the Isolation of TI-plasmid from Agrobacterium Tumefaciens.” NUCLEIC ACIDS RESEARCH 3.2 (1976): 449–463. Print.
APA
Ledeboer, A. M., Krol, A., Dons, J. J., Spier, F., Schilperoort, R. A., Zaenen, I., Van Larebeke, N., et al. (1976). On the isolation of TI-plasmid from Agrobacterium tumefaciens. NUCLEIC ACIDS RESEARCH, 3(2), 449–463.
Chicago author-date
Ledeboer, Adrianus M, AJM Krol, Johannes JM Dons, F Spier, Robert A Schilperoort, Ivo Zaenen, Nicolas Van Larebeke, and Jeff Schell. 1976. “On the Isolation of TI-plasmid from Agrobacterium Tumefaciens.” Nucleic Acids Research 3 (2): 449–463.
Chicago author-date (all authors)
Ledeboer, Adrianus M, AJM Krol, Johannes JM Dons, F Spier, Robert A Schilperoort, Ivo Zaenen, Nicolas Van Larebeke, and Jeff Schell. 1976. “On the Isolation of TI-plasmid from Agrobacterium Tumefaciens.” Nucleic Acids Research 3 (2): 449–463.
Vancouver
1.
Ledeboer AM, Krol A, Dons JJ, Spier F, Schilperoort RA, Zaenen I, et al. On the isolation of TI-plasmid from Agrobacterium tumefaciens. NUCLEIC ACIDS RESEARCH. 1976;3(2):449–63.
IEEE
[1]
A. M. Ledeboer et al., “On the isolation of TI-plasmid from Agrobacterium tumefaciens,” NUCLEIC ACIDS RESEARCH, vol. 3, no. 2, pp. 449–463, 1976.
@article{6857894,
  abstract     = {{An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA, followed by Sarkosyl, after growth in the presence of carbenicillin. We also present a procedure for the isolation of the TI-plasmid on a large scale, that might be used for the mass isolation of other large plasmids which like the TI-plasmid, can not be cleared with earlier described procedures. The purity of the plasmid preparations was determined with DNA renaturation kinetics, which method has the advantage that the plasmid need not to be in the supercoiled or open circular form.}},
  author       = {{Ledeboer, Adrianus M and Krol, AJM and Dons, Johannes JM and Spier, F and Schilperoort, Robert A and Zaenen, Ivo and Van Larebeke, Nicolas and Schell, Jeff}},
  issn         = {{0305-1048}},
  journal      = {{NUCLEIC ACIDS RESEARCH}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{449--463}},
  title        = {{On the isolation of TI-plasmid from Agrobacterium tumefaciens}},
  url          = {{http://dx.doi.org/10.1093/nar/3.2.449}},
  volume       = {{3}},
  year         = {{1976}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: