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Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation

Annelies Suetens, Marjan Moreels, Roel Quintens, Els Soors, Jasmine Buset, Sabina Chiriotti, Kevin Tabury, Vincent Grégoire and Sarah Baatout (UGent)
(2015) JOURNAL OF RADIATION RESEARCH. 56(1). p.11-21
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Abstract
Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness (RBE). This high ballistic accuracy of particle beams deposits the maximal dose to the tumor, while damage to the surrounding healthy tissue is limited. Currently, hadrontherapy is being used for the treatment of specific types of cancer. Previous in vitro studies have shown that, under certain circumstances, exposure to charged particles may inhibit cell motility and migration. In the present study, we investigated the expression of four motility-related genes in prostate (PC3) and colon (Caco-2) cancer cell lines after exposure to different radiation types. Cells were irradiated with various absorbed doses (0, 0.5 and 2 Gy) of accelerated C-13-ions at the GANIL facility (Caen, France) or with X-rays. Clonogenic assays were performed to determine the RBE. RT-qPCR analysis showed dose- and time-dependent changes in the expression of CCDC88A, FN1, MYH9 and ROCK1 in both cell lines. However, whereas in PC3 cells the response to carbon ion irradiation was enhanced compared with X-irradiation, the effect was the opposite in Caco-2 cells, indicating cell-type-specific responses to the different radiation types.
Keywords
Caco-2 colon adenocarcinoma, gene expression, PC3 prostate adenocarcinoma, motility genes, carbon ion irradiation, colony survival assay, IONIZING-RADIATION, PROMOTES MIGRATION, HUMAN GLIOBLASTOMA, BEAM IRRADIATION, LET RADIATION, UP-REGULATION, GLIOMA-CELLS, INVASION, METASTASIS, LINES

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MLA
Suetens, Annelies, et al. “Dose- and Time-Dependent Gene Expression Alterations in Prostate and Colon Cancer Cells after in Vitro Exposure to Carbon Ion and X-Irradiation.” JOURNAL OF RADIATION RESEARCH, vol. 56, no. 1, 2015, pp. 11–21, doi:10.1093/jrr/rru070.
APA
Suetens, A., Moreels, M., Quintens, R., Soors, E., Buset, J., Chiriotti, S., … Baatout, S. (2015). Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation. JOURNAL OF RADIATION RESEARCH, 56(1), 11–21. https://doi.org/10.1093/jrr/rru070
Chicago author-date
Suetens, Annelies, Marjan Moreels, Roel Quintens, Els Soors, Jasmine Buset, Sabina Chiriotti, Kevin Tabury, Vincent Grégoire, and Sarah Baatout. 2015. “Dose- and Time-Dependent Gene Expression Alterations in Prostate and Colon Cancer Cells after in Vitro Exposure to Carbon Ion and X-Irradiation.” JOURNAL OF RADIATION RESEARCH 56 (1): 11–21. https://doi.org/10.1093/jrr/rru070.
Chicago author-date (all authors)
Suetens, Annelies, Marjan Moreels, Roel Quintens, Els Soors, Jasmine Buset, Sabina Chiriotti, Kevin Tabury, Vincent Grégoire, and Sarah Baatout. 2015. “Dose- and Time-Dependent Gene Expression Alterations in Prostate and Colon Cancer Cells after in Vitro Exposure to Carbon Ion and X-Irradiation.” JOURNAL OF RADIATION RESEARCH 56 (1): 11–21. doi:10.1093/jrr/rru070.
Vancouver
1.
Suetens A, Moreels M, Quintens R, Soors E, Buset J, Chiriotti S, et al. Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation. JOURNAL OF RADIATION RESEARCH. 2015;56(1):11–21.
IEEE
[1]
A. Suetens et al., “Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation,” JOURNAL OF RADIATION RESEARCH, vol. 56, no. 1, pp. 11–21, 2015.
@article{6855099,
  abstract     = {{Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness (RBE). This high ballistic accuracy of particle beams deposits the maximal dose to the tumor, while damage to the surrounding healthy tissue is limited. Currently, hadrontherapy is being used for the treatment of specific types of cancer. Previous in vitro studies have shown that, under certain circumstances, exposure to charged particles may inhibit cell motility and migration. In the present study, we investigated the expression of four motility-related genes in prostate (PC3) and colon (Caco-2) cancer cell lines after exposure to different radiation types. Cells were irradiated with various absorbed doses (0, 0.5 and 2 Gy) of accelerated C-13-ions at the GANIL facility (Caen, France) or with X-rays. Clonogenic assays were performed to determine the RBE. RT-qPCR analysis showed dose- and time-dependent changes in the expression of CCDC88A, FN1, MYH9 and ROCK1 in both cell lines. However, whereas in PC3 cells the response to carbon ion irradiation was enhanced compared with X-irradiation, the effect was the opposite in Caco-2 cells, indicating cell-type-specific responses to the different radiation types.}},
  author       = {{Suetens, Annelies and Moreels, Marjan and Quintens, Roel and Soors, Els and Buset, Jasmine and Chiriotti, Sabina and Tabury, Kevin and Grégoire, Vincent and Baatout, Sarah}},
  issn         = {{0449-3060}},
  journal      = {{JOURNAL OF RADIATION RESEARCH}},
  keywords     = {{Caco-2 colon adenocarcinoma,gene expression,PC3 prostate adenocarcinoma,motility genes,carbon ion irradiation,colony survival assay,IONIZING-RADIATION,PROMOTES MIGRATION,HUMAN GLIOBLASTOMA,BEAM IRRADIATION,LET RADIATION,UP-REGULATION,GLIOMA-CELLS,INVASION,METASTASIS,LINES}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{11--21}},
  title        = {{Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation}},
  url          = {{http://doi.org/10.1093/jrr/rru070}},
  volume       = {{56}},
  year         = {{2015}},
}
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