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Enzymatic degumming: degumming efficiency versus yield increase

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Abstract
Enzymatic degumming trials were performed on crude extracted soybean (SBO) and rapeseed oils (RSO) with a microbial phospholipase A(1) (Lecitase Ultra (R)). We obtained a degummed oil with a phosphorus content<10mg/kg when applying an enzyme dosage of 30mg/kg and (feedstock dependent) a contact time of 10-120min. While a good degumming efficiency can already be obtained after a relative short reaction time, it was observed that a longer reaction time (1-2h) is required for complete degradation of the phospholipids (PL), which results in a yield increase. We demonstrated that Lecitase Ultra (R) has no specificity for a given PL, but that the rate of conversion depends on the PL composition of the crude oil. After 60min, 80% of phosphatidyl ethanolamine (PE) was degraded to its lyso-form while only 40% of phosphatidyl inositol (PI) was converted in the same time interval. The formation of glycerophospholipids (GPL) was only observed after 60min reaction. Practical applications: Practical advantages and disadvantages of the use of Lecithase Ultra for enzymatic degumming of oils is described, focusing on soybean oil and rapeseed oil. The information will be valuable and important for the oil refining industry.
Keywords
Phospholipid composition, Phospholipase A(1), OPTIMIZATION, ULTRA, PHOSPHOLIPASE, SOYBEAN OIL, Oil degumming, Enzymatic activity, Vegetable oils

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MLA
Araujo Sampaio, Klicia et al. “Enzymatic Degumming: Degumming Efficiency Versus Yield Increase.” EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 117.1 (2015): 81–86. Print.
APA
Araujo Sampaio, K., Zyaykina, N., Wozniak, B., Tsukamoto, J., De Greyt, W., & Stevens, C. (2015). Enzymatic degumming: degumming efficiency versus yield increase. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 117(1), 81–86.
Chicago author-date
Araujo Sampaio, Klicia, Nadezhda Zyaykina, Bogumila Wozniak, Junko Tsukamoto, Wim De Greyt, and Christian Stevens. 2015. “Enzymatic Degumming: Degumming Efficiency Versus Yield Increase.” European Journal of Lipid Science and Technology 117 (1): 81–86.
Chicago author-date (all authors)
Araujo Sampaio, Klicia, Nadezhda Zyaykina, Bogumila Wozniak, Junko Tsukamoto, Wim De Greyt, and Christian Stevens. 2015. “Enzymatic Degumming: Degumming Efficiency Versus Yield Increase.” European Journal of Lipid Science and Technology 117 (1): 81–86.
Vancouver
1.
Araujo Sampaio K, Zyaykina N, Wozniak B, Tsukamoto J, De Greyt W, Stevens C. Enzymatic degumming: degumming efficiency versus yield increase. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. 2015;117(1):81–6.
IEEE
[1]
K. Araujo Sampaio, N. Zyaykina, B. Wozniak, J. Tsukamoto, W. De Greyt, and C. Stevens, “Enzymatic degumming: degumming efficiency versus yield increase,” EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, vol. 117, no. 1, pp. 81–86, 2015.
@article{6855073,
  abstract     = {Enzymatic degumming trials were performed on crude extracted soybean (SBO) and rapeseed oils (RSO) with a microbial phospholipase A(1) (Lecitase Ultra (R)). We obtained a degummed oil with a phosphorus content<10mg/kg when applying an enzyme dosage of 30mg/kg and (feedstock dependent) a contact time of 10-120min. While a good degumming efficiency can already be obtained after a relative short reaction time, it was observed that a longer reaction time (1-2h) is required for complete degradation of the phospholipids (PL), which results in a yield increase. We demonstrated that Lecitase Ultra (R) has no specificity for a given PL, but that the rate of conversion depends on the PL composition of the crude oil. After 60min, 80% of phosphatidyl ethanolamine (PE) was degraded to its lyso-form while only 40% of phosphatidyl inositol (PI) was converted in the same time interval. The formation of glycerophospholipids (GPL) was only observed after 60min reaction. 
Practical applications: Practical advantages and disadvantages of the use of Lecithase Ultra for enzymatic degumming of oils is described, focusing on soybean oil and rapeseed oil. The information will be valuable and important for the oil refining industry.},
  author       = {Araujo Sampaio, Klicia and Zyaykina, Nadezhda and Wozniak, Bogumila and Tsukamoto, Junko and De Greyt, Wim and Stevens, Christian},
  issn         = {1438-7697},
  journal      = {EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY},
  keywords     = {Phospholipid composition,Phospholipase A(1),OPTIMIZATION,ULTRA,PHOSPHOLIPASE,SOYBEAN OIL,Oil degumming,Enzymatic activity,Vegetable oils},
  language     = {eng},
  number       = {1},
  pages        = {81--86},
  title        = {Enzymatic degumming: degumming efficiency versus yield increase},
  url          = {http://dx.doi.org/10.1002/ejlt.201400218},
  volume       = {117},
  year         = {2015},
}

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