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Surgical injury to the mouse pancreas through ligation of the pancreatic duct as a model for endocrine and exocrine reprogramming and proliferation

Sofie De Groef, Gunter Leuckx, Naomi Van Gassen, Willem Staels UGent, Ying Cai, Yuchi Yuchi, Violette Coppens, Nico De Leu, Yves Heremans, Luc Baeyens, et al. (2015) JOVE-JOURNAL OF VISUALIZED EXPERIMENTS.
abstract
Expansion of pancreatic beta cells in vivo or ex vivo, or generation of beta cells by differentiation from an embryonic or adult stem cell, can provide new expandable sources of beta cells to alleviate the donor scarcity in human islet transplantation as therapy for diabetes. Although recent advances have been made towards this aim, mechanisms that regulate beta cell expansion and differentiation from a stem/progenitor cell remain to be characterized. Here, we describe a protocol for an injury model in the adult mouse pancreas that can function as a tool to study mechanisms of tissue remodeling and beta cell proliferation and differentiation. Partial duct ligation (PDL) is an experimentally induced injury of the rodent pancreas involving surgical ligation of the main pancreatic duct resulting in an obstruction of drainage of exocrine products out of the tail region of the pancreas. The inflicted damage induces acinar atrophy, immune cell infiltration and severe tissue remodeling. We have previously reported the activation of Neurogenin (Ngn) 3 expressing endogenous progenitor-like cells and an increase in beta cell proliferation after PDL. Therefore, PDL provides a basis to study signals involved in beta cell dynamics and the properties of an endocrine progenitor in adult pancreas. Since, it still remains largely unclear, which factors and pathways contribute to beta cell neogenesis and proliferation in PDL, a standardized protocol for PDL will allow for comparison across laboratories.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DIFFERENTIATION, MICE, REGENERATION, PROGENITORS, ISLET GROWTH, ADULT PANCREAS, BETA-CELL NEOGENESIS, CONTRIBUTE, EXPRESSION, INVOLVE, cell reprogramming, cell proliferation, Neurogenin (Ngn) 3, mouse model, cell differentiation, injury, surgery, Partial duct ligation (PDL), Pancreas, Issue 102, Medicine
journal title
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
J. Vis. Exp.
issue
102
article number
e52765
pages
9 pages
Web of Science type
Article
Web of Science id
000361537100010
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
1.113 (2015)
JCR rank
24/63 (2015)
JCR quartile
2 (2015)
ISSN
1940-087X
DOI
10.3791/52765
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6854376
handle
http://hdl.handle.net/1854/LU-6854376
date created
2015-06-30 15:08:21
date last changed
2016-12-21 15:41:23
@article{6854376,
  abstract     = {Expansion of pancreatic beta cells in vivo or ex vivo, or generation of beta cells by differentiation from an embryonic or adult stem cell, can provide new expandable sources of beta cells to alleviate the donor scarcity in human islet transplantation as therapy for diabetes. Although recent advances have been made towards this aim, mechanisms that regulate beta cell expansion and differentiation from a stem/progenitor cell remain to be characterized. Here, we describe a protocol for an injury model in the adult mouse pancreas that can function as a tool to study mechanisms of tissue remodeling and beta cell proliferation and differentiation. Partial duct ligation (PDL) is an experimentally induced injury of the rodent pancreas involving surgical ligation of the main pancreatic duct resulting in an obstruction of drainage of exocrine products out of the tail region of the pancreas. The inflicted damage induces acinar atrophy, immune cell infiltration and severe tissue remodeling. We have previously reported the activation of Neurogenin (Ngn) 3 expressing endogenous progenitor-like cells and an increase in beta cell proliferation after PDL. Therefore, PDL provides a basis to study signals involved in beta cell dynamics and the properties of an endocrine progenitor in adult pancreas. Since, it still remains largely unclear, which factors and pathways contribute to beta cell neogenesis and proliferation in PDL, a standardized protocol for PDL will allow for comparison across laboratories.},
  articleno    = {e52765},
  author       = {De Groef, Sofie and Leuckx, Gunter and Van Gassen, Naomi and Staels, Willem and Cai, Ying and Yuchi, Yuchi and Coppens, Violette and De Leu, Nico and Heremans, Yves and Baeyens, Luc and Van de Casteele, Mark and Heimberg, Harry},
  issn         = {1940-087X},
  journal      = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS},
  keyword      = {DIFFERENTIATION,MICE,REGENERATION,PROGENITORS,ISLET GROWTH,ADULT PANCREAS,BETA-CELL NEOGENESIS,CONTRIBUTE,EXPRESSION,INVOLVE,cell reprogramming,cell proliferation,Neurogenin (Ngn) 3,mouse model,cell differentiation,injury,surgery,Partial duct ligation (PDL),Pancreas,Issue 102,Medicine},
  language     = {eng},
  number       = {102},
  pages        = {9},
  title        = {Surgical injury to the mouse pancreas through ligation of the pancreatic duct as a model for endocrine and exocrine reprogramming and proliferation},
  url          = {http://dx.doi.org/10.3791/52765},
  year         = {2015},
}

Chicago
De Groef, Sofie, Gunter Leuckx, Naomi Van Gassen, Willem Staels, Ying Cai, Yuchi Yuchi, Violette Coppens, et al. 2015. “Surgical Injury to the Mouse Pancreas Through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation.” Jove-journal of Visualized Experiments (102).
APA
De Groef, S., Leuckx, G., Van Gassen, N., Staels, W., Cai, Y., Yuchi, Y., Coppens, V., et al. (2015). Surgical injury to the mouse pancreas through ligation of the pancreatic duct as a model for endocrine and exocrine reprogramming and proliferation. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (102).
Vancouver
1.
De Groef S, Leuckx G, Van Gassen N, Staels W, Cai Y, Yuchi Y, et al. Surgical injury to the mouse pancreas through ligation of the pancreatic duct as a model for endocrine and exocrine reprogramming and proliferation. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2015;(102).
MLA
De Groef, Sofie, Gunter Leuckx, Naomi Van Gassen, et al. “Surgical Injury to the Mouse Pancreas Through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation.” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 102 (2015): n. pag. Print.