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Laser-induced vapor nanobubbles for efficient delivery of macromolecules in live cells

Ranhua Xiong (UGent) , Koen Raemdonck (UGent) , Karen Peynshaert (UGent) , Ine Lentacker (UGent) , Ine De Cock (UGent) , Jo Demeester (UGent) , Stefaan De Smedt (UGent) , Andre Skirtach (UGent) and Kevin Braeckmans (UGent)
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Center for nano- and biophotonics (NB-Photonics)
Abstract
Macromolecular agents such as nucleic acids and proteins need to be delivered into living cells for therapeutic purposes. Among physical methods to deliver macromolecules across the cell membrane, laser- induced photoporation using plasmonic nanoparticles is a method that is receiving increasing attention in recent years. By irradiating gold nanoparticles bound to the cell membrane with laser light, nanosized membrane pores can be created. Pores are formed by localized heating or by vapour nanobubbles (VNBs) depending on the incident laser energy. Macromolecules in the surrounding cell medium can then diffuse through the transiently formed pores into the cytoplasm. While both heating and VNBs have been reported before for permeabilization of the cell membrane, it remains unclear which of both methods is more efficient in terms of cell loading with minimal cytotoxicity. In this study we report that under condition of a single 7 ns laser pulse VNBs are substantially more efficient for the cytosolic delivery of macromolecules. We conclude that VNB formation is an interesting photoporation mechanism for fast and efficient macromolecular delivery in live cells.
Keywords
gold nanoparticles, vapour nanobubbles, photoporation, intracellular delivery, macromolecules, BUBBLES, NANOPARTICLES

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Chicago
Xiong, Ranhua, Koen Raemdonck, Karen Peynshaert, Ine Lentacker, Ine De Cock, Jo Demeester, Stefaan De Smedt, Andre Skirtach, and Kevin Braeckmans. 2015. “Laser-induced Vapor Nanobubbles for Efficient Delivery of Macromolecules in Live Cells.” In Proceedings of SPIE, ed. WJ Parak, M Osinski, and XJ Liang. Vol. 9338. Bellingham, WA, USA: SPIE-International Society for Optical Engineering.
APA
Xiong, R., Raemdonck, K., Peynshaert, K., Lentacker, I., De Cock, I., Demeester, J., De Smedt, S., et al. (2015). Laser-induced vapor nanobubbles for efficient delivery of macromolecules in live cells. In W. Parak, M. Osinski, & X. Liang (Eds.), Proceedings of SPIE (Vol. 9338). Presented at the Conference on Colloidal Nanoparticles for Biomedical Applications X, Bellingham, WA, USA: SPIE-International Society for Optical Engineering.
Vancouver
1.
Xiong R, Raemdonck K, Peynshaert K, Lentacker I, De Cock I, Demeester J, et al. Laser-induced vapor nanobubbles for efficient delivery of macromolecules in live cells. In: Parak W, Osinski M, Liang X, editors. Proceedings of SPIE. Bellingham, WA, USA: SPIE-International Society for Optical Engineering; 2015.
MLA
Xiong, Ranhua, Koen Raemdonck, Karen Peynshaert, et al. “Laser-induced Vapor Nanobubbles for Efficient Delivery of Macromolecules in Live Cells.” Proceedings of SPIE. Ed. WJ Parak, M Osinski, & XJ Liang. Vol. 9338. Bellingham, WA, USA: SPIE-International Society for Optical Engineering, 2015. Print.
@inproceedings{6854259,
  abstract     = {Macromolecular agents such as nucleic acids and proteins need to be delivered into living cells for therapeutic purposes. Among physical methods to deliver macromolecules across the cell membrane, laser- induced photoporation using plasmonic nanoparticles is a method that is receiving increasing attention in recent years. By irradiating gold nanoparticles bound to the cell membrane with laser light, nanosized membrane pores can be created. Pores are formed by localized heating or by vapour nanobubbles (VNBs) depending on the incident laser energy. Macromolecules in the surrounding cell medium can then diffuse through the transiently formed pores into the cytoplasm. While both heating and VNBs have been reported before for permeabilization of the cell membrane, it remains unclear which of both methods is more efficient in terms of cell loading with minimal cytotoxicity. In this study we report that under condition of a single 7 ns laser pulse VNBs are substantially more efficient for the cytosolic delivery of macromolecules. We conclude that VNB formation is an interesting photoporation mechanism for fast and efficient macromolecular delivery in live cells.},
  articleno    = {93381J},
  author       = {Xiong, Ranhua and Raemdonck, Koen and Peynshaert, Karen and Lentacker, Ine and De Cock, Ine and Demeester, Jo and De Smedt, Stefaan and Skirtach, Andre and Braeckmans, Kevin},
  booktitle    = {Proceedings of SPIE},
  editor       = {Parak, WJ and Osinski, M and Liang, XJ},
  isbn         = {9781628414288},
  issn         = {0277-786X},
  language     = {eng},
  location     = {San Francisco, CA, USA},
  pages        = {7},
  publisher    = {SPIE-International Society for Optical Engineering},
  title        = {Laser-induced vapor nanobubbles for efficient delivery of macromolecules in live cells},
  url          = {http://dx.doi.org/10.1117/12.2078895},
  volume       = {9338},
  year         = {2015},
}

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