Advanced search
1 file | 2.27 MB

Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation

Ranhua Xiong (UGent) , Freya Joris (UGent) , Ine De Cock (UGent) , Jo Demeester (UGent) , Stefaan De Smedt (UGent) , Andre Skirtach (UGent) and Kevin Braeckmans (UGent)
Author
Organization
Project
Center for nano- and biophotonics (NB-Photonics)
Abstract
There is considerable interest in using Quantum Dots (QDs) as fluorescent probes such for cellular imaging due to unique advantages in comparison with conventional molecular dyes. However, cytosolic delivery of QDs into live cells remains a major challenge. Here we demonstrate highly efficient delivery of PEG-coated QDs into live cells by means of laser-induced vapour nanobubbles. Using this procedure we succeeded in high-throughput loading of similar to 80% of cells while maintaining a cell viability of similar to 85%
Keywords
QDs, photoporation, vapor nanobubble, gold nanoparticle, LABELS, NANOBUBBLES

Downloads

  • Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation.docx
    • full text
    • |
    • open access
    • |
    • Word
    • |
    • 2.27 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Xiong, Ranhua, Freya Joris, Ine De Cock, Jo Demeester, Stefaan De Smedt, Andre Skirtach, and Kevin Braeckmans. 2015. “Efficient Delivery of Quantum Dots in Live Cells by Gold Nanoparticle Mediated Photoporation.” In Proceedings of SPIE, ed. WJ Parak, M Osinski, and XJ Liang. Vol. 9338. Bellingham, WA, USA: SPIE-International Society for Optical Engineering.
APA
Xiong, R., Joris, F., De Cock, I., Demeester, J., De Smedt, S., Skirtach, A., & Braeckmans, K. (2015). Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation. In W. Parak, M. Osinski, & X. Liang (Eds.), Proceedings of SPIE (Vol. 9338). Presented at the Conference on Colloidal Nanoparticles for Biomedical Applications X, Bellingham, WA, USA: SPIE-International Society for Optical Engineering.
Vancouver
1.
Xiong R, Joris F, De Cock I, Demeester J, De Smedt S, Skirtach A, et al. Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation. In: Parak W, Osinski M, Liang X, editors. Proceedings of SPIE. Bellingham, WA, USA: SPIE-International Society for Optical Engineering; 2015.
MLA
Xiong, Ranhua, Freya Joris, Ine De Cock, et al. “Efficient Delivery of Quantum Dots in Live Cells by Gold Nanoparticle Mediated Photoporation.” Proceedings of SPIE. Ed. WJ Parak, M Osinski, & XJ Liang. Vol. 9338. Bellingham, WA, USA: SPIE-International Society for Optical Engineering, 2015. Print.
@inproceedings{6854234,
  abstract     = {There is considerable interest in using Quantum Dots (QDs) as fluorescent probes such for cellular imaging due to unique advantages in comparison with conventional molecular dyes. However, cytosolic delivery of QDs into live cells remains a major challenge. Here we demonstrate highly efficient delivery of PEG-coated QDs into live cells by means of laser-induced vapour nanobubbles. Using this procedure we succeeded in high-throughput loading of similar to 80\% of cells while maintaining a cell viability of similar to 85\%},
  articleno    = {93380X},
  author       = {Xiong, Ranhua and Joris, Freya and De Cock, Ine and Demeester, Jo and De Smedt, Stefaan and Skirtach, Andre and Braeckmans, Kevin},
  booktitle    = {Proceedings of SPIE},
  editor       = {Parak, WJ and Osinski, M and Liang, XJ},
  isbn         = {9781628414288},
  issn         = {0277-786X},
  language     = {eng},
  location     = {San Francisco, CA, USA},
  pages        = {5},
  publisher    = {SPIE-International Society for Optical Engineering},
  title        = {Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation},
  url          = {http://dx.doi.org/10.1117/12.2075659},
  volume       = {9338},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: