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Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)

Jan M van der Wolf, Els H Nijhuis, Malgorzata J Kowalewska, Gerry S Saddler, Neil Parkinson, John G Elphinstone, Leighton Pritchard, Ian K Toth, Ewa Lojkowska, Marta Potrykus, et al. (2014) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 64(3). p.768-774
abstract
Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi, on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from alpha-methylglucoside. Differential physiological assays used previously to differentiate between strains of E chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rpIB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222(T)=LMG25993(T)=NCPP134479(T)) is proposed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CHRYSANTHEMI, RELATEDNESS, PSEUDOMALLEI, IDENTIFICATION, STRAINS, CLONALITY, MALLEI, CLADE, GENUS
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
64
issue
3
pages
768 - 774
Web of Science type
Article
Web of Science id
000337930900012
JCR category
MICROBIOLOGY
JCR impact factor
2.511 (2014)
JCR rank
60/119 (2014)
JCR quartile
3 (2014)
ISSN
1466-5026
DOI
10.1099/ijs.0.052944-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6852640
handle
http://hdl.handle.net/1854/LU-6852640
date created
2015-06-29 22:33:36
date last changed
2016-12-19 15:43:33
@article{6852640,
  abstract     = {Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi, on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from alpha-methylglucoside. Differential physiological assays used previously to differentiate between strains of E chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rpIB, rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222(T)=LMG25993(T)=NCPP134479(T)) is proposed.},
  author       = {van der Wolf, Jan M and Nijhuis, Els H and Kowalewska, Malgorzata J and Saddler, Gerry S and Parkinson, Neil and Elphinstone, John G and Pritchard, Leighton and Toth, Ian K and Lojkowska, Ewa and Potrykus, Marta and Waleron, Margorzata and De Vos, Paul and Cleenwerck, Ilse and Pirhonen, Minna and Garlant, Linda and H{\'e}lias, Val{\'e}rie and Pothier, Jo{\"e}l F and Pfl{\"u}ger, Valentin and Duffy, Brion and Tsror, Leah and Manulis, Shula},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {CHRYSANTHEMI,RELATEDNESS,PSEUDOMALLEI,IDENTIFICATION,STRAINS,CLONALITY,MALLEI,CLADE,GENUS},
  language     = {eng},
  number       = {3},
  pages        = {768--774},
  title        = {Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)},
  url          = {http://dx.doi.org/10.1099/ijs.0.052944-0},
  volume       = {64},
  year         = {2014},
}

Chicago
van der Wolf, Jan M, Els H Nijhuis, Malgorzata J Kowalewska, Gerry S Saddler, Neil Parkinson, John G Elphinstone, Leighton Pritchard, et al. 2014. “Dickeya Solani Sp. Nov., a Pectinolytic Plant-pathogenic Bacterium Isolated from Potato (Solanum Tuberosum).” International Journal of Systematic and Evolutionary Microbiology 64 (3): 768–774.
APA
van der Wolf, J. M., Nijhuis, E. H., Kowalewska, M. J., Saddler, G. S., Parkinson, N., Elphinstone, J. G., Pritchard, L., et al. (2014). Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(3), 768–774.
Vancouver
1.
van der Wolf JM, Nijhuis EH, Kowalewska MJ, Saddler GS, Parkinson N, Elphinstone JG, et al. Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum). INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(3):768–74.
MLA
van der Wolf, Jan M, Els H Nijhuis, Malgorzata J Kowalewska, et al. “Dickeya Solani Sp. Nov., a Pectinolytic Plant-pathogenic Bacterium Isolated from Potato (Solanum Tuberosum).” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.3 (2014): 768–774. Print.