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The cellular interactions of PEGylated gold nanoparticles : effect of PEGylation on cellular uptake and cytotoxicity

Stefaan Soenen, Bella B Manshian, Abuelmagd M Abdelmonem, José-María Montenegro, Sisareuth Tan, Lieve Balcaen UGent, Frank Vanhaecke UGent, Alain R Brisson, Wolfgang J Parak, Stefaan De Smedt UGent, et al. (2014) PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION. 31(7). p.794-800
abstract
Poly(ethylene glycol) (PEG) is frequently used to coat various medical nanoparticles (NPs). As PEG is known to minimize NP interactions with biological specimens, the question remains whether PEGylated NPs are intrinsically less toxic or whether this is caused by reduced NP uptake. In the present work, the effect of gold NP PEGylation on uptake by three cell types is compared and evaluated the effect on cell viability, oxidative stress, cell morphology, and functionality using a multiparametric methodology. The data reveal that PEGylation affects cellular NP uptake in a cell-type-dependent manner and influences toxicity by different mechanisms. At similar intracellular NP numbers, PEGylated NPs are found to yield higher levels of cell death, mostly by induction of oxidative stress. These findings reveal that PEGylation significantly reduces NP uptake, but that at similar functional (= cell-associated) NP levels, non-PEGylated NPs are better tolerated by the cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EXPOSURE, DESIGN, NANOTOXICITY, FUNCTIONALIZATION, CELLS, TOXICITY, POLYMER, COLLOIDAL NANOPARTICLES, COATED QUANTUM DOTS, POLY(ETHYLENE GLYCOL)
journal title
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
Part. Part. Syst. Charact.
volume
31
issue
7
pages
794 - 800
Web of Science type
Article
Web of Science id
000340670600009
JCR category
MATERIALS SCIENCE, MULTIDISCIPLINARY
JCR impact factor
3.081 (2014)
JCR rank
47/260 (2014)
JCR quartile
1 (2014)
ISSN
0934-0866
DOI
10.1002/ppsc.201300357
project
Center for nano- and biophotonics (NB-Photonics)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6852091
handle
http://hdl.handle.net/1854/LU-6852091
date created
2015-06-29 14:31:59
date last changed
2018-01-29 12:19:11
@article{6852091,
  abstract     = {Poly(ethylene glycol) (PEG) is frequently used to coat various medical nanoparticles (NPs). As PEG is known to minimize NP interactions with biological specimens, the question remains whether PEGylated NPs are intrinsically less toxic or whether this is caused by reduced NP uptake. In the present work, the effect of gold NP PEGylation on uptake by three cell types is compared and evaluated the effect on cell viability, oxidative stress, cell morphology, and functionality using a multiparametric methodology. The data reveal that PEGylation affects cellular NP uptake in a cell-type-dependent manner and influences toxicity by different mechanisms. At similar intracellular NP numbers, PEGylated NPs are found to yield higher levels of cell death, mostly by induction of oxidative stress. These findings reveal that PEGylation significantly reduces NP uptake, but that at similar functional (= cell-associated) NP levels, non-PEGylated NPs are better tolerated by the cells.},
  author       = {Soenen, Stefaan and Manshian, Bella B and Abdelmonem, Abuelmagd M and Montenegro, Jos{\'e}-Mar{\'i}a and Tan, Sisareuth and Balcaen, Lieve and Vanhaecke, Frank and Brisson, Alain R and Parak, Wolfgang J and De Smedt, Stefaan and Braeckmans, Kevin},
  issn         = {0934-0866},
  journal      = {PARTICLE \& PARTICLE SYSTEMS CHARACTERIZATION},
  keyword      = {EXPOSURE,DESIGN,NANOTOXICITY,FUNCTIONALIZATION,CELLS,TOXICITY,POLYMER,COLLOIDAL NANOPARTICLES,COATED QUANTUM DOTS,POLY(ETHYLENE GLYCOL)},
  language     = {eng},
  number       = {7},
  pages        = {794--800},
  title        = {The cellular interactions of PEGylated gold nanoparticles : effect of PEGylation on cellular uptake and cytotoxicity},
  url          = {http://dx.doi.org/10.1002/ppsc.201300357},
  volume       = {31},
  year         = {2014},
}

Chicago
Soenen, Stefaan, Bella B Manshian, Abuelmagd M Abdelmonem, José-María Montenegro, Sisareuth Tan, Lieve Balcaen, Frank Vanhaecke, et al. 2014. “The Cellular Interactions of PEGylated Gold Nanoparticles : Effect of PEGylation on Cellular Uptake and Cytotoxicity.” Particle & Particle Systems Characterization 31 (7): 794–800.
APA
Soenen, S., Manshian, B. B., Abdelmonem, A. M., Montenegro, J.-M., Tan, S., Balcaen, L., Vanhaecke, F., et al. (2014). The cellular interactions of PEGylated gold nanoparticles : effect of PEGylation on cellular uptake and cytotoxicity. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION, 31(7), 794–800.
Vancouver
1.
Soenen S, Manshian BB, Abdelmonem AM, Montenegro J-M, Tan S, Balcaen L, et al. The cellular interactions of PEGylated gold nanoparticles : effect of PEGylation on cellular uptake and cytotoxicity. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION. 2014;31(7):794–800.
MLA
Soenen, Stefaan, Bella B Manshian, Abuelmagd M Abdelmonem, et al. “The Cellular Interactions of PEGylated Gold Nanoparticles : Effect of PEGylation on Cellular Uptake and Cytotoxicity.” PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION 31.7 (2014): 794–800. Print.