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Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae

Ut Nguyen Van UGent, Vesna Melkebeek, Bert Devriendt UGent, Tiphanie Goetstouwers, Mario Van Poucke UGent, Luc Peelman UGent, Bruno Goddeeris and Eric Cox UGent (2015) VETERINARY RESEARCH. 46.
abstract
F4 enterotoxigenic Escherichia coli (ETEC) cause diarrhoea and mortality in piglets leading to severe economic losses. Oral immunization of piglets with F4 fimbriae induces a protective intestinal immune response evidenced by an F4-specific serum and intestinal IgA response. However, successful oral immunization of pigs with F4 fimbriae in the presence of maternal immunity has not been demonstrated yet. In the present study we aimed to evaluate the effect of maternal immunity on the induction of a systemic immune response upon oral immunization of piglets. Whereas F4-specific IgG and IgA could be induced by oral immunization of pigs without maternal antibodies and by intramuscular immunization of pigs with maternal antibodies, no such response was seen in the orally immunized animals with maternal antibodies. Since maternal antibodies can mask an antibody response, we also looked by ELIspot assays for circulating F4-specific antibody secreting cells (ASCs). Enumerating the F4-specific ASCs within the circulating peripheral blood mononuclear cells, and the number of F4-specific IgA ASCs within the circulating IgA+ B-cells revealed an F4-specific immune response in the orally immunized animals with maternal antibodies. Interestingly, results suggest a more robust IgA booster response by oral immunization of pigs with than without maternal antibodies. These results demonstrate that oral immunization of piglets with F4-specific maternal antibodies is feasible and that these maternal antibodies seem to enhance the secondary systemic immune response. Furthermore, our ELIspot assay on enriched IgA+ B-cells could be used as a screening procedure to optimize mucosal immunization protocols in pigs with maternal immunity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
F4 ETEC, maternal immunity, oral immunisation, pigs, ELIspot, NEONATAL FC-RECEPTOR, ENTEROTOXIGENIC ESCHERICHIA-COLI, DENDRITIC CELLS, ELISPOT ASSAY, PURIFIED F4, IGG, BINDING, PIGS, DIFFERENTIATION, IMMUNOGLOBULIN
journal title
VETERINARY RESEARCH
Vet. Res.
volume
46
article number
72
pages
8 pages
Web of Science type
Article
Web of Science id
000356590700001
JCR category
VETERINARY SCIENCES
JCR impact factor
2.928 (2015)
JCR rank
3/138 (2015)
JCR quartile
1 (2015)
ISSN
0928-4249
DOI
10.1186/s13567-015-0210-3
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
6851374
handle
http://hdl.handle.net/1854/LU-6851374
date created
2015-06-29 10:31:16
date last changed
2016-12-21 15:42:32
@article{6851374,
  abstract     = {F4 enterotoxigenic Escherichia coli (ETEC) cause diarrhoea and mortality in piglets leading to severe economic losses. Oral immunization of piglets with F4 fimbriae induces a protective intestinal immune response evidenced by
an F4-specific serum and intestinal IgA response. However, successful oral immunization of pigs with F4 fimbriae in the presence of maternal immunity has not been demonstrated yet. In the present study we aimed to evaluate the effect of maternal immunity on the induction of a systemic immune response upon oral immunization of piglets. Whereas F4-specific IgG and IgA could be induced by oral immunization of pigs without maternal antibodies
and by intramuscular immunization of pigs with maternal antibodies, no such response was seen in the orally immunized animals with maternal antibodies. Since maternal antibodies can mask an antibody response, we also looked by ELIspot assays for circulating F4-specific antibody secreting cells (ASCs). Enumerating the F4-specific ASCs within the circulating peripheral blood mononuclear cells, and the number of F4-specific IgA ASCs within the circulating IgA+ B-cells revealed an F4-specific immune response in the orally immunized animals with maternal antibodies. Interestingly, results suggest a more robust IgA booster response by oral immunization of pigs with than without maternal antibodies. These results demonstrate that oral immunization of piglets with F4-specific maternal antibodies is feasible and that these maternal antibodies seem to enhance the secondary systemic immune response. Furthermore, our ELIspot assay on enriched IgA+ B-cells could be used as a screening procedure to optimize mucosal immunization protocols in pigs with maternal immunity.},
  articleno    = {72},
  author       = {Nguyen Van, Ut and Melkebeek, Vesna and Devriendt, Bert and Goetstouwers, Tiphanie and Van Poucke, Mario and Peelman, Luc and Goddeeris, Bruno and Cox, Eric},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {F4 ETEC,maternal immunity,oral immunisation,pigs,ELIspot,NEONATAL FC-RECEPTOR,ENTEROTOXIGENIC ESCHERICHIA-COLI,DENDRITIC CELLS,ELISPOT ASSAY,PURIFIED F4,IGG,BINDING,PIGS,DIFFERENTIATION,IMMUNOGLOBULIN},
  language     = {eng},
  pages        = {8},
  title        = {Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae},
  url          = {http://dx.doi.org/10.1186/s13567-015-0210-3},
  volume       = {46},
  year         = {2015},
}

Chicago
Nguyen Van, Ut, Vesna Melkebeek, Bert Devriendt, Tiphanie Goetstouwers, Mario Van Poucke, Luc Peelman, Bruno Goddeeris, and Eric Cox. 2015. “Maternal Immunity Enhances Systemic Recall Immune Responses Upon Oral Immunization of Piglets with F4 Fimbriae.” Veterinary Research 46.
APA
Nguyen Van, U., Melkebeek, V., Devriendt, B., Goetstouwers, T., Van Poucke, M., Peelman, L., Goddeeris, B., et al. (2015). Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae. VETERINARY RESEARCH, 46.
Vancouver
1.
Nguyen Van U, Melkebeek V, Devriendt B, Goetstouwers T, Van Poucke M, Peelman L, et al. Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae. VETERINARY RESEARCH. 2015;46.
MLA
Nguyen Van, Ut, Vesna Melkebeek, Bert Devriendt, et al. “Maternal Immunity Enhances Systemic Recall Immune Responses Upon Oral Immunization of Piglets with F4 Fimbriae.” VETERINARY RESEARCH 46 (2015): n. pag. Print.