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Antimicrobial resistance and population structure of methicillin-resistant Staphylococcus aureus recovered from pigs

Laura Peeters UGent, MA Argudín, Sonya Azadikhah and Patrick Butaye UGent (2015) Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, 4th ASM conference, Abstracts.
abstract
Three-hundred twenty-eight Belgian farms were sampled for MRSA in 2013. Per farm, 20 pooled nose swabs from 20 animals were analysed by selective enrichment and plating on MRSA selective plate, MRSA-ID (bioMérieux, France) as described before (Nemeghaire et al., 2013). One MRSA strain per farm was further analysed by MRSA identification and typing (spa-typing, sau1-hsdS1 clonal complex (CC) 398 PCR, MLST, SCCmec typing, microarray analysis (Identibac S. aureus Genotyping DNA micro array, Alere Technologies GmbH, Germany), and antimicrobial susceptibility testing was done as described before (Nemeghaire et al., 2013). MRSA was present in 65.6% (95% CI: 60.1%-71%) of the 328 farms sampled. Most isolates (n=205) were positive for the sau1-hsdS1 CC398 PCR. The remaining eight isolates were ST9 (one isolate), ST80 (two isolates), ST239 (one isolate) or ST398 (six isolates). spa types t044, t337 and t4150 were found in the CC80, CC9 and CC8 isolates, respectively. Nineteen spa types were found among the 211 CC398 isolates. Most strains were spa type t011 (n=180). The other types found were t034, t1344, t1451, t1456, t1580, t1985, t2123, t2370, t2922, t3171, t3424, t3854, t4432, t4872, t5452, t5051, t6628, t8100. The isolates belonged to CC8, CC9 and CC80 carried SCCmec cassettes types IVc or V. Most CC398 isolates carried SCCmec V (79%), and less carried SCCmec III (1%) or IVa (18%). A total of 4 isolates carried NT cassettes, one ST239 and 3 CC398. Most strains had additional resistance to 5 antibiotics. Micro array analysis showed a great variety in resistance genes and genes encoding virulence factors including haemolysins, proteases, biofilm production, adhesion factors, immune-evasion factors, the intact hlbgene, putative transport proteins and staphylococcal superantigen-like proteins from the vSaα genomic island. Capsule 5-related genes, the egc-like cluster, were less prevalent. Non CC398 strains carried additional virulence characteristics like leukocidins (lukED, lukPV), an exfoliatin (etd), and an epidermal cell differentiation inhibitor (edinB). The sau1-hsdS1 clonal complex (CC) 398 PCR did not detect all CC398 strains in our hands, however it remains a good screening method. The prevalence of MRSA in Belgium seems to be stable over time, comparing results going back to 2006. The diversity of CC398 seems to have increased, with the appearance of multiple spa types within CC398 and MLST types other than CC398. ST 239, a sequence type belonging to the healthcare-associated CC8 clonal complex, was found in pigs as it was found before in bovines and poultry in Belgium. The presence of other MRSA (and possibly also MSSA) offers the possibility to the LA-MRSA CC398 acquiring more virulence and resistance genes. This is a potential risk for human and animal health given the possibility of this strain to spread to different animal species.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, 4th ASM conference, Abstracts
publisher
American Society for Microbiology (ASM)
conference name
4th ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens
conference location
Washington, DC, USA
conference start
2015-05-08
conference end
2015-05-11
language
English
UGent publication?
yes
classification
C3
id
6847971
handle
http://hdl.handle.net/1854/LU-6847971
date created
2015-06-24 17:28:09
date last changed
2016-12-19 15:35:51
@inproceedings{6847971,
  abstract     = {Three-hundred twenty-eight Belgian farms were sampled for MRSA in 2013. Per farm, 20 pooled nose swabs from 20 animals were analysed by selective enrichment and plating on MRSA selective plate, MRSA-ID (bioM{\'e}rieux, France) as described before (Nemeghaire et al., 2013). One MRSA strain per farm was further analysed by MRSA identification and typing (spa-typing, sau1-hsdS1 clonal complex (CC) 398 PCR, MLST, SCCmec typing, microarray analysis (Identibac S. aureus Genotyping DNA micro array, Alere Technologies GmbH, Germany), and antimicrobial susceptibility testing was done as described before (Nemeghaire et al., 2013).
MRSA was present in 65.6\% (95\% CI: 60.1\%-71\%) of the 328 farms sampled. Most isolates (n=205) were positive for the sau1-hsdS1 CC398 PCR. The remaining eight isolates were ST9 (one isolate), ST80 (two isolates), ST239 (one isolate) or ST398 (six isolates).
spa types t044, t337 and t4150 were found in the CC80, CC9 and CC8 isolates, respectively. Nineteen spa types were found among the 211 CC398 isolates. Most strains were spa type t011 (n=180). The other types found were t034, t1344, t1451, t1456, t1580, t1985, t2123, t2370, t2922, t3171, t3424, t3854, t4432, t4872, t5452, t5051, t6628, t8100. The isolates belonged to CC8, CC9 and CC80 carried SCCmec cassettes types IVc or V. Most CC398 isolates carried SCCmec V (79\%), and less carried SCCmec III (1\%) or IVa (18\%). A total of 4 isolates carried NT cassettes, one ST239 and 3 CC398. Most strains had additional resistance to 5 antibiotics. Micro array analysis showed a great variety in resistance genes and genes encoding virulence factors including haemolysins, proteases, biofilm production, adhesion factors, immune-evasion factors, the intact hlbgene, putative transport proteins and staphylococcal superantigen-like proteins from the vSa\ensuremath{\alpha} genomic island. Capsule 5-related genes, the egc-like cluster, were less prevalent. Non CC398 strains carried additional virulence characteristics like leukocidins (lukED, lukPV), an exfoliatin (etd), and an epidermal cell differentiation inhibitor (edinB).
The sau1-hsdS1 clonal complex (CC) 398 PCR did not detect all CC398 strains in our hands, however it remains a good screening method. The prevalence of MRSA in Belgium seems to be stable over time, comparing results going back to 2006. The diversity of CC398 seems to have increased, with the appearance of multiple spa types within CC398 and MLST types other than CC398. ST 239, a sequence type belonging to the healthcare-associated CC8 clonal complex, was found in pigs as it was found before in bovines and poultry in Belgium. The presence of other MRSA (and possibly also MSSA) offers the possibility to the LA-MRSA CC398 acquiring more virulence and resistance genes. This is a potential risk for human and animal health given the possibility of this strain to spread to different animal species.},
  author       = {Peeters, Laura and Argud{\'i}n, MA and Azadikhah, Sonya and Butaye, Patrick},
  booktitle    = {Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, 4th ASM conference, Abstracts},
  language     = {eng},
  location     = {Washington, DC, USA},
  publisher    = {American Society for Microbiology (ASM)},
  title        = {Antimicrobial resistance and population structure of methicillin-resistant Staphylococcus aureus recovered from pigs},
  year         = {2015},
}

Chicago
Peeters, Laura, MA Argudín, Sonya Azadikhah, and Patrick Butaye. 2015. “Antimicrobial Resistance and Population Structure of Methicillin-resistant Staphylococcus Aureus Recovered from Pigs.” In Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, 4th ASM Conference, Abstracts. American Society for Microbiology (ASM).
APA
Peeters, Laura, Argudín, M., Azadikhah, S., & Butaye, P. (2015). Antimicrobial resistance and population structure of methicillin-resistant Staphylococcus aureus recovered from pigs. Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, 4th ASM conference, Abstracts. Presented at the 4th ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, American Society for Microbiology (ASM).
Vancouver
1.
Peeters L, Argudín M, Azadikhah S, Butaye P. Antimicrobial resistance and population structure of methicillin-resistant Staphylococcus aureus recovered from pigs. Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, 4th ASM conference, Abstracts. American Society for Microbiology (ASM); 2015.
MLA
Peeters, Laura, MA Argudín, Sonya Azadikhah, et al. “Antimicrobial Resistance and Population Structure of Methicillin-resistant Staphylococcus Aureus Recovered from Pigs.” Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens, 4th ASM Conference, Abstracts. American Society for Microbiology (ASM), 2015. Print.