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Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system

(2015) ENVIRONMENTAL MICROBIOLOGY. 17(5). p.1586-1599
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Abstract
Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favourable osmotic conditions. Cell wall stress such as exposure to -lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the inducing agent. We have adopted Escherichia coli as a model system for the controlled transition to and reversion from the L-form state, and have studied these dynamics with genetics, cell biology and omics' technologies. As such, a transposon mutagenesis screen underscored the requirement for the Rcs phosphorelay and colanic acid synthesis, while proteomics show only little differences between rods and L-forms. In contrast, metabolome comparison reveals the high abundance of lysophospholipids and phospholipids with unsaturated or cyclopropanized fatty acids in E.coliL-forms. This increase of membrane lipids associated with increased membrane fluidity may facilitate proliferation through bud formation. Visualization of the residual peptidoglycan with a fluorescently labelled peptidoglycan binding protein indicates de novo cell wall synthesis and a role for septal peptidoglycan synthesis during bud constriction. The DD-carboxypeptidases PBP5 and PBP6 are threefold and fourfold upregulated in L-forms, indicating a specific role for regulation of crosslinking during L-form proliferation.
Keywords
PROLIFERATION, PROTEINS, GROWTH, DIVISION, EXPRESSION, RESISTANCE, STRESS-RESPONSE, CYCLOPROPANE RING, L-FORMS, BACILLUS-SUBTILIS

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Please use this url to cite or link to this publication:

Chicago
Cambré, Alexander, Michael Zimmermann, Uwe Sauer, Bram Vivijs, William Cenens, Chris W Michiels, Abram Aertsen, et al. 2015. “Metabolite Profiling and Peptidoglycan Analysis of Transient Cell Wall-deficient Bacteria in a New Escherichia Coli Model System.” Environmental Microbiology 17 (5): 1586–1599.
APA
Cambré, A., Zimmermann, M., Sauer, U., Vivijs, B., Cenens, W., Michiels, C. W., Aertsen, A., et al. (2015). Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system. ENVIRONMENTAL MICROBIOLOGY, 17(5), 1586–1599.
Vancouver
1.
Cambré A, Zimmermann M, Sauer U, Vivijs B, Cenens W, Michiels CW, et al. Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system. ENVIRONMENTAL MICROBIOLOGY. 2015;17(5):1586–99.
MLA
Cambré, Alexander, Michael Zimmermann, Uwe Sauer, et al. “Metabolite Profiling and Peptidoglycan Analysis of Transient Cell Wall-deficient Bacteria in a New Escherichia Coli Model System.” ENVIRONMENTAL MICROBIOLOGY 17.5 (2015): 1586–1599. Print.
@article{6846319,
  abstract     = {Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favourable osmotic conditions. Cell wall stress such as exposure to -lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the inducing agent. We have adopted Escherichia coli as a model system for the controlled transition to and reversion from the L-form state, and have studied these dynamics with genetics, cell biology and omics' technologies. As such, a transposon mutagenesis screen underscored the requirement for the Rcs phosphorelay and colanic acid synthesis, while proteomics show only little differences between rods and L-forms. In contrast, metabolome comparison reveals the high abundance of lysophospholipids and phospholipids with unsaturated or cyclopropanized fatty acids in E.coliL-forms. This increase of membrane lipids associated with increased membrane fluidity may facilitate proliferation through bud formation. Visualization of the residual peptidoglycan with a fluorescently labelled peptidoglycan binding protein indicates de novo cell wall synthesis and a role for septal peptidoglycan synthesis during bud constriction. The DD-carboxypeptidases PBP5 and PBP6 are threefold and fourfold upregulated in L-forms, indicating a specific role for regulation of crosslinking during L-form proliferation.},
  author       = {Cambr{\'e}, Alexander and Zimmermann, Michael and Sauer, Uwe and Vivijs, Bram and Cenens, William and Michiels, Chris W and Aertsen, Abram and Loessner, Martin J and Noben, Jean-Paul and Ayala, Juan A and Lavigne, Rob and Briers, Yves},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {1586--1599},
  title        = {Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system},
  url          = {http://dx.doi.org/10.1111/1462-2920.12594},
  volume       = {17},
  year         = {2015},
}

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