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Evaluation of hydrophilic interaction chromatography versus reversed-phase chromatography in a plant metabolomics perspective

Ruben T'Kindt UGent, Michael Storme, Dieter Deforce UGent and Jan Van Bocxlaer UGent (2008) JOURNAL OF SEPARATION SCIENCE. 31(9). p.1609-1614
abstract
The metabolomics goal, the unbiased relative quantification of all metabolites in a biological system, still lacks a universal analytical approach. In the LC-MS line of approach, one of the major problems encountered is the polar nature of a large group of (plant) metabolites. Here, we investigate the potential of hydrophilic interaction chromatography (HILIC) and compare its qualities with extended polarity RP chromatography. Two opposite LC phase compositions (Atlantis dC18 vs. TSKgel Amide-80) are compared in a plant metabolomics setting. Both performed equally well with regard to retentive capacities, but variation in peak area was about 5% higher for the HILIC approach. Focussing on matrix effects (ME) on the other hand, it was observed that this well-known problem in RP LC-MS metabolomics was not reduced on using hydrophilic interaction chromatography.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FLIGHT MASS-SPECTROMETRY, RAT URINE, METABOLITES, METABONOMICS, extended polarity reversed-phase material, plant metabolomics, microliquid chromatography, mass spectrometry, hydrophilic interaction chromatography (HILIC), PERFORMANCE LIQUID-CHROMATOGRAPHY
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
31
issue
9
pages
1609 - 1614
Web of Science type
Article
Web of Science id
000256535800019
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.746 (2008)
JCR rank
17/70 (2008)
JCR quartile
1 (2008)
ISSN
1615-9306
DOI
10.1002/jssc.200700539
language
English
UGent publication?
yes
classification
A1
id
684413
handle
http://hdl.handle.net/1854/LU-684413
date created
2009-06-09 10:24:30
date last changed
2009-09-22 14:25:12
@article{684413,
  abstract     = {The metabolomics goal, the unbiased relative quantification of all metabolites in a biological system, still lacks a universal analytical approach. In the LC-MS line of approach, one of the major problems encountered is the polar nature of a large group of (plant) metabolites. Here, we investigate the potential of hydrophilic interaction chromatography (HILIC) and compare its qualities with extended polarity RP chromatography. Two opposite LC phase compositions (Atlantis dC18 vs. TSKgel Amide-80) are compared in a plant metabolomics setting. Both performed equally well with regard to retentive capacities, but variation in peak area was about 5\% higher for the HILIC approach. Focussing on matrix effects (ME) on the other hand, it was observed that this well-known problem in RP LC-MS metabolomics was not reduced on using hydrophilic interaction chromatography.},
  author       = {T'Kindt, Ruben and Storme, Michael and Deforce, Dieter and Van Bocxlaer, Jan},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {FLIGHT MASS-SPECTROMETRY,RAT URINE,METABOLITES,METABONOMICS,extended polarity reversed-phase material,plant metabolomics,microliquid chromatography,mass spectrometry,hydrophilic interaction chromatography (HILIC),PERFORMANCE LIQUID-CHROMATOGRAPHY},
  language     = {eng},
  number       = {9},
  pages        = {1609--1614},
  title        = {Evaluation of hydrophilic interaction chromatography versus reversed-phase chromatography in a plant metabolomics perspective},
  url          = {http://dx.doi.org/10.1002/jssc.200700539},
  volume       = {31},
  year         = {2008},
}

Chicago
T’Kindt, Ruben, Michael Storme, Dieter Deforce, and Jan Van Bocxlaer. 2008. “Evaluation of Hydrophilic Interaction Chromatography Versus Reversed-phase Chromatography in a Plant Metabolomics Perspective.” Journal of Separation Science 31 (9): 1609–1614.
APA
T’Kindt, R., Storme, M., Deforce, D., & Van Bocxlaer, J. (2008). Evaluation of hydrophilic interaction chromatography versus reversed-phase chromatography in a plant metabolomics perspective. JOURNAL OF SEPARATION SCIENCE, 31(9), 1609–1614.
Vancouver
1.
T’Kindt R, Storme M, Deforce D, Van Bocxlaer J. Evaluation of hydrophilic interaction chromatography versus reversed-phase chromatography in a plant metabolomics perspective. JOURNAL OF SEPARATION SCIENCE. 2008;31(9):1609–14.
MLA
T’Kindt, Ruben, Michael Storme, Dieter Deforce, et al. “Evaluation of Hydrophilic Interaction Chromatography Versus Reversed-phase Chromatography in a Plant Metabolomics Perspective.” JOURNAL OF SEPARATION SCIENCE 31.9 (2008): 1609–1614. Print.