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Integrated community profiling indicates long-term temporal stability of the predominant faecal microbiota in captive cheetahs

Anne Becker (UGent) , Geert Janssens (UGent) , Cindy Snauwaert (UGent) , Myriam Hesta (UGent) and Geert Huys (UGent)
(2015) PLOS ONE. 10(4).
Author
Organization
Abstract
Understanding the symbiotic relationship between gut microbes and their animal host re- quires characterization of the core microbiota across populations and in time. Especially in captive populations of endangered wildlife species such as the cheetah (Acinonyx jubatus), this knowledge is a key element to enhance feeding strategies and reduce gastrointestinal disorders. In order to investigate the temporal stability of the intestinal microbiota in cheetahs under human care, we conducted a longitudinal study over a 3-year period with bimonthly faecal sampling of 5 cheetahs housed in two European zoos. For this purpose, an integrated 16S rRNA DGGE-clone library approach was used in combination with a series of real-time PCR assays. Our findings disclosed a stable faecal microbiota, beyond intestinal community variations that were detected between zoo sample sets or between animals. The core of this microbiota was dominated by members of Clostridium clusters I, XI and XIVa, with mean concentrations ranging from 7.5-9.2 log10 CFU/g faeces and with significant positive correla- tions between these clusters (P<0.05), and by Lactobacillaceae. Moving window analysis of DGGE profiles revealed 23.3-25.6% change between consecutive samples for four of the cheetahs. The fifth animal in the study suffered from intermediate episodes of vomiting and diarrhea during the monitoring period and exhibited remarkably more change (39.4%). This observation may reflect the temporary impact of perturbations such as the animal’s compro- mised health, antibiotic administration or a combination thereof, which temporarily altered the relative proportions of Clostridium clusters I and XIVa. In conclusion, this first long-term monitoring study of the faecal microbiota in feline strict carnivores not only reveals a remark- able compositional stability of this ecosystem, but also shows a qualitative and quantitative similarity in a defined set of faecal bacterial lineages across the five animals under study that may typify the core phylogenetic microbiome of cheetahs.
Keywords
VERTEBRATE GUT MICROBIOTA, GRADIENT GEL-ELECTROPHORESIS, REAL-TIME PCR, 16S RIBOSOMAL-RNA, microbial stability, cheetah, community fingerprinting, carnivore, gut microbiota, CLOSTRIDIUM-PERFRINGENS, ACINONYX-JUBATUS, INTESTINAL MICROBIOTA, BACTERIAL-POPULATIONS, PROBIOTIC PRODUCTS, PANTHERA-TIGRIS

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Citation

Please use this url to cite or link to this publication:

Chicago
Becker, Anne, Geert Janssens, Cindy Snauwaert, Myriam Hesta, and Geert Huys. 2015. “Integrated Community Profiling Indicates Long-term Temporal Stability of the Predominant Faecal Microbiota in Captive Cheetahs.” Plos One 10 (4).
APA
Becker, Anne, Janssens, G., Snauwaert, C., Hesta, M., & Huys, G. (2015). Integrated community profiling indicates long-term temporal stability of the predominant faecal microbiota in captive cheetahs. PLOS ONE, 10(4).
Vancouver
1.
Becker A, Janssens G, Snauwaert C, Hesta M, Huys G. Integrated community profiling indicates long-term temporal stability of the predominant faecal microbiota in captive cheetahs. PLOS ONE. 2015;10(4).
MLA
Becker, Anne, Geert Janssens, Cindy Snauwaert, et al. “Integrated Community Profiling Indicates Long-term Temporal Stability of the Predominant Faecal Microbiota in Captive Cheetahs.” PLOS ONE 10.4 (2015): n. pag. Print.
@article{6841948,
  abstract     = {Understanding the symbiotic relationship between gut microbes and their animal host re- quires characterization of the core microbiota across populations and in time. Especially in captive populations of endangered wildlife species such as the cheetah (Acinonyx jubatus), this knowledge is a key element to enhance feeding strategies and reduce gastrointestinal disorders. In order to investigate the temporal stability of the intestinal microbiota in cheetahs under human care, we conducted a longitudinal study over a 3-year period with bimonthly faecal sampling of 5 cheetahs housed in two European zoos. For this purpose, an integrated 16S rRNA DGGE-clone library approach was used in combination with a series of real-time PCR assays. Our findings disclosed a stable faecal microbiota, beyond intestinal community variations that were detected between zoo sample sets or between animals. The core of this microbiota was dominated by members of Clostridium clusters I, XI and XIVa, with mean concentrations ranging from 7.5-9.2 log10 CFU/g faeces and with significant positive correla- tions between these clusters (P{\textlangle}0.05), and by Lactobacillaceae. Moving window analysis of DGGE profiles revealed 23.3-25.6\% change between consecutive samples for four of the cheetahs. The fifth animal in the study suffered from intermediate episodes of vomiting and diarrhea during the monitoring period and exhibited remarkably more change (39.4\%). This observation may reflect the temporary impact of perturbations such as the animal{\textquoteright}s compro- mised health, antibiotic administration or a combination thereof, which temporarily altered the relative proportions of Clostridium clusters I and XIVa. In conclusion, this first long-term monitoring study of the faecal microbiota in feline strict carnivores not only reveals a remark- able compositional stability of this ecosystem, but also shows a qualitative and quantitative similarity in a defined set of faecal bacterial lineages across the five animals under study that may typify the core phylogenetic microbiome of cheetahs.},
  articleno    = {e0123933},
  author       = {Becker, Anne and Janssens, Geert and Snauwaert, Cindy and Hesta, Myriam and Huys, Geert},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {4},
  pages        = {19},
  title        = {Integrated community profiling indicates long-term temporal stability of the predominant faecal microbiota in captive cheetahs},
  url          = {http://dx.doi.org/10.1371/journal.pone.0123933},
  volume       = {10},
  year         = {2015},
}

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