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Synthetic biology devices and circuits for RNA-based 'smart vaccines': a propositional review

(2015) EXPERT REVIEW OF VACCINES. 14(2). p.313-331
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Abstract
Nucleic acid vaccines have been gaining attention as an alternative to the standard attenuated pathogen or protein based vaccine. However, an unrealized advantage of using such DNA or RNA based vaccination modalities is the ability to program within these nucleic acids regulatory devices that would provide an immunologist with the power to control the production of antigens and adjuvants in a desirable manner by administering small molecule drugs as chemical triggers. Advances in synthetic biology have resulted in the creation of highly predictable and modular genetic parts and devices that can be composed into synthetic gene circuits with complex behaviors. With the recent advent of modified RNA gene delivery methods and developments in the RNA replicon platform, we foresee a future in which mammalian synthetic biologists will create genetic circuits encoded exclusively on RNA. Here, we review the current repertoire of devices used in RNA synthetic biology and propose how programmable 'smart vaccines' will revolutionize the field of RNA vaccination.
Keywords
modified RNA, destabilizing domains, post-transcriptional gene regulation, RNA, RNA binding proteins, RNA replicons, RNAi, synthetic biology, synthetic gene circuits, synthetic riboswitches, vaccination, MODIFIED MESSENGER-RNA, MAMMALIAN-CELLS, GENE-EXPRESSION, TRANSLATIONAL REGULATION, RATIONAL DESIGN, IMMUNE-RESPONSE, SMALL MOLECULES, PUF PROTEINS, STRUCTURAL REQUIREMENTS, TRANSGENE EXPRESSION

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Citation

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Chicago
Andries, Oliwia, Tasuku Kitada, Katie Bodner, Niek Sanders, and Ron Weiss. 2015. “Synthetic Biology Devices and Circuits for RNA-based ‘Smart Vaccines’: a Propositional Review.” Expert Review of Vaccines 14 (2): 313–331.
APA
Andries, O., Kitada, T., Bodner, K., Sanders, N., & Weiss, R. (2015). Synthetic biology devices and circuits for RNA-based “smart vaccines”: a propositional review. EXPERT REVIEW OF VACCINES, 14(2), 313–331.
Vancouver
1.
Andries O, Kitada T, Bodner K, Sanders N, Weiss R. Synthetic biology devices and circuits for RNA-based “smart vaccines”: a propositional review. EXPERT REVIEW OF VACCINES. 2015;14(2):313–31.
MLA
Andries, Oliwia et al. “Synthetic Biology Devices and Circuits for RNA-based ‘Smart Vaccines’: a Propositional Review.” EXPERT REVIEW OF VACCINES 14.2 (2015): 313–331. Print.
@article{6839332,
  abstract     = {Nucleic acid vaccines have been gaining attention as an alternative to the standard attenuated pathogen or protein based vaccine. However, an unrealized advantage of using such DNA or RNA based vaccination modalities is the ability to program within these nucleic acids regulatory devices that would provide an immunologist with the power to control the production of antigens and adjuvants in a desirable manner by administering small molecule drugs as chemical triggers. Advances in synthetic biology have resulted in the creation of highly predictable and modular genetic parts and devices that can be composed into synthetic gene circuits with complex behaviors. With the recent advent of modified RNA gene delivery methods and developments in the RNA replicon platform, we foresee a future in which mammalian synthetic biologists will create genetic circuits encoded exclusively on RNA. Here, we review the current repertoire of devices used in RNA synthetic biology and propose how programmable 'smart vaccines' will revolutionize the field of RNA vaccination.},
  author       = {Andries, Oliwia and Kitada, Tasuku and Bodner, Katie and Sanders, Niek and Weiss, Ron},
  issn         = {1476-0584},
  journal      = {EXPERT REVIEW OF VACCINES},
  keywords     = {modified RNA,destabilizing domains,post-transcriptional gene regulation,RNA,RNA binding proteins,RNA replicons,RNAi,synthetic biology,synthetic gene circuits,synthetic riboswitches,vaccination,MODIFIED MESSENGER-RNA,MAMMALIAN-CELLS,GENE-EXPRESSION,TRANSLATIONAL REGULATION,RATIONAL DESIGN,IMMUNE-RESPONSE,SMALL MOLECULES,PUF PROTEINS,STRUCTURAL REQUIREMENTS,TRANSGENE EXPRESSION},
  language     = {eng},
  number       = {2},
  pages        = {313--331},
  title        = {Synthetic biology devices and circuits for RNA-based 'smart vaccines': a propositional review},
  url          = {http://dx.doi.org/10.1586/14760584.2015.997714},
  volume       = {14},
  year         = {2015},
}

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