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Application of poly-β-hydroxybutyrate accumulating bacteria in crustacean larviculture

(2015)
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Abstract
This PhD research was first illustrated that the use of amorphous PHB contained in whole cells of the bacterium Alcaligenes eutrophus in lyophilized and freshly grown cell forms induced similar and even better beneficial effects on the growth performance or disease resistance of Artemia nauplii or M. rosenbergii larvae than crystalline PHB particles. In the second part, the change in carbonaceous compounds (trehalose, glycerol, glycogen and total organic carbon) and total nitrogen in the axenic hatching medium of decapsulated Artemia fransicana cysts was determined throughout the hatching process. Three different salinities (5, 12 and 35 g/L) of the incubation medium were applied. Trehalose appeared in the medium in small quantities (maximally 2.6 mg C/g incubated dry cysts) as compared to glycerol and glycogen (maximally 28.5 ± 1.2 and 13.8 ± 1.0 mg C/g incubated dry cysts, respectively). Overall, the C/N ratio in Artemia hatching medium at a salinity of 12 g/L (which is most relevant for practice) was about 10 throughout incubation. In the final part of this work, the reuse of the hatching medium of Artemia was investigated as a cost-efficient strategy to culture the PHB accumulating bacterium Bacillus sp. LT12 and supply it to Artemia nauplii or M. rosenbergii laviculture as an antimicrobial agent. The PHB level in Bacillus sp. LT12 was increased about 2-fold when this bacterium was cultured in the axenic hatching medium of Artemia (AHMA) harvested at the different times points during the incubation process (16, 20 & 24 h). Adding the bacterium which was cultured in AHMA harvested at 16 or 20 h, into the culture water of Artemia nauplii challenged with Vibrio campbellii LMG21363 showed to completely protect the nauplii when they were added at a density of 5 x 107 CFU/mL. Moreover, the disease resistance of M. rosenbergii larvae in the challenge test with Vibrio harveyi BB120 was significantly increased when feeding Artemia nauplii enriched with 109 CFU/mL of Bacillus sp. LT12 grown as well in the hatching medium of Artemia harvested at 16, 20 or 24 h. When Bacillus sp. LT12 was co-cultured with Artemia during cyst incubation and supplementary glycerol was added at 0.17 and 0.51 g/L, the results showed that the PHB content in the bacteria and the disease resistance of M. rosenbergii larvae were significantly increased as compared to control treatment (without adding extra glycerol). The results in this PhD work illustrate that whole bacterial cells containing amorphous PHB are more promising to use as a disease control strategy in crustacean larviculture than crystalline PHB. The reuse of Artemia hatching medium in crustacean hatcheries as the nutrient medium to culture PHB accumulating bacteria to be fed to the cultured animals does not only result in an increased culture efficiency of the larvae but also reduces the load on the environment by reducing the volume of waste water originating from crustacean hatcheries.

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MLA
Thai, Truong-Quoc. Application of Poly-β-Hydroxybutyrate Accumulating Bacteria in Crustacean Larviculture. Ghent University. Faculty of Bioscience Engineering, 2015.
APA
Thai, T.-Q. (2015). Application of poly-β-hydroxybutyrate accumulating bacteria in crustacean larviculture. Ghent University. Faculty of Bioscience Engineering, Ghent, Belgium.
Chicago author-date
Thai, Truong-Quoc. 2015. “Application of Poly-β-Hydroxybutyrate Accumulating Bacteria in Crustacean Larviculture.” Ghent, Belgium: Ghent University. Faculty of Bioscience Engineering.
Chicago author-date (all authors)
Thai, Truong-Quoc. 2015. “Application of Poly-β-Hydroxybutyrate Accumulating Bacteria in Crustacean Larviculture.” Ghent, Belgium: Ghent University. Faculty of Bioscience Engineering.
Vancouver
1.
Thai T-Q. Application of poly-β-hydroxybutyrate accumulating bacteria in crustacean larviculture. [Ghent, Belgium]: Ghent University. Faculty of Bioscience Engineering; 2015.
IEEE
[1]
T.-Q. Thai, “Application of poly-β-hydroxybutyrate accumulating bacteria in crustacean larviculture,” Ghent University. Faculty of Bioscience Engineering, Ghent, Belgium, 2015.
@phdthesis{6838700,
  abstract     = {{This PhD research was first illustrated that the use of amorphous PHB contained in whole cells of the bacterium Alcaligenes eutrophus in lyophilized and freshly grown cell forms induced similar and even better beneficial effects on the growth performance or disease resistance of Artemia nauplii or M. rosenbergii larvae than crystalline PHB particles. In the second part, the change in carbonaceous compounds (trehalose, glycerol, glycogen and total organic carbon) and total nitrogen in the axenic hatching medium of decapsulated Artemia fransicana cysts was determined throughout the hatching process. Three different salinities (5, 12 and 35 g/L) of the incubation medium were applied. Trehalose appeared in the medium in small quantities (maximally 2.6 mg C/g incubated dry cysts) as compared to glycerol and glycogen (maximally 28.5 ± 1.2 and 13.8 ± 1.0 mg C/g incubated dry cysts, respectively). Overall, the C/N ratio in Artemia hatching medium at a salinity of 12 g/L (which is most relevant for practice) was about 10 throughout incubation. In the final part of this work, the reuse of the hatching medium of Artemia was investigated as a cost-efficient strategy to culture the PHB accumulating bacterium Bacillus sp. LT12 and supply it to Artemia nauplii or M. rosenbergii laviculture as an antimicrobial agent. The PHB level in Bacillus sp. LT12 was increased about 2-fold when this bacterium was cultured in the axenic hatching medium of Artemia (AHMA) harvested at the different times points during the incubation process (16, 20 & 24 h). Adding the bacterium which was cultured in AHMA harvested at 16 or 20 h, into the culture water of Artemia nauplii challenged with Vibrio campbellii LMG21363 showed to completely protect the nauplii when they were added at a density of 5 x 107 CFU/mL. Moreover, the disease resistance of M. rosenbergii larvae in the challenge test with Vibrio harveyi BB120 was significantly increased when feeding Artemia nauplii enriched with 109 CFU/mL of Bacillus sp. LT12 grown as well in the hatching medium of Artemia harvested at 16, 20 or 24 h. When Bacillus sp. LT12 was co-cultured with Artemia during cyst incubation and supplementary glycerol was added at 0.17 and 0.51 g/L, the results showed that the PHB content in the bacteria and the disease resistance of M. rosenbergii larvae were significantly increased as compared to control treatment (without adding extra glycerol). The results in this PhD work illustrate that whole bacterial cells containing amorphous PHB are more promising to use as a disease control strategy in crustacean larviculture than crystalline PHB. The reuse of Artemia hatching medium in crustacean hatcheries as the nutrient medium to culture PHB accumulating bacteria to be fed to the cultured animals does not only result in an increased culture efficiency of the larvae but also reduces the load on the environment by reducing the volume of waste water originating from crustacean hatcheries.}},
  author       = {{Thai, Truong-Quoc}},
  isbn         = {{9789059898080}},
  language     = {{eng}},
  pages        = {{VIII, 224}},
  publisher    = {{Ghent University. Faculty of Bioscience Engineering}},
  school       = {{Ghent University}},
  title        = {{Application of poly-β-hydroxybutyrate accumulating bacteria in crustacean larviculture}},
  year         = {{2015}},
}