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Folates from metabolically engineered rice: a long-term study in rats

Filip Kiekens (UGent) , Dieter Blancquaert (UGent) , Lindsey Devisscher (UGent) , Jeroen Van Daele (UGent) , Veronique Stove (UGent) , Joris Delanghe (UGent) , Dominique Van Der Straeten (UGent) , Willy Lambert (UGent) and Christophe Stove (UGent)
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Abstract
Scope: The biological impact of folates from folate rice, a metabolically engineered (biofortified) rice line, rich in folates, was investigated. Its consumption may be helpful to fight folate deficiency. Our objective was to investigate the potential of folate rice to supply the organism with folates and evaluate its biological effectiveness using a rat model. Methods and results: Five groups of 12 Wistar rats were monitored during a 7/12-wk depletion/repletion trial. Animals receiving folate-free diet (0 mu g/rat/day) and those additionally receiving wild-type rice (on average 0.11 mu g/rat/day) suffered from decreased hematocrit and lower folate concentrations in both plasma and RBCs. This resulted in serious morbidity and even lethality during the trial. In contrast, all animals receiving a daily supplement of folate rice or folic acid fortified rice (on average 3.00 mu g/rat/day and 3.12 mu g/rat/day, respectively) and those receiving a positive control diet (11.4 to 25.0 mu g/rat/day), survived. In these groups, the hematocrit normalized, plasma and RBC folate concentrations increased and pronounced hyperhomocysteinemia was countered. Conclusion: Using an animal model, we demonstrated that biofortified folate rice is a valuable source of dietary folate, as evidenced by folate determination in plasma and RBCs, the alleviation of anemia and counteraction of pronounced hyperhomocysteinemia.
Keywords
INTESTINAL-ABSORPTION, Bioactivity, PLASMA HOMOCYSTEINE, CHEMISTRY ANALYZER, BIOAVAILABILITY, FOOD, FORTIFICATION, IMPACT, CARDIOVASCULAR-DISEASE, Folate, Biofortification, BIOFORTIFIED RICE, Rice, FOLIC-ACID, Murine model

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Chicago
Kiekens, Filip, Dieter Blancquaert, Lindsey Devisscher, Jeroen Van Daele, Veronique Stove, Joris Delanghe, Dominique Van Der Straeten, Willy Lambert, and Christophe Stove. 2015. “Folates from Metabolically Engineered Rice: a Long-term Study in Rats.” Molecular Nutrition & Food Research 59 (3): 490–500.
APA
Kiekens, F., Blancquaert, D., Devisscher, L., Van Daele, J., Stove, V., Delanghe, J., Van Der Straeten, D., et al. (2015). Folates from metabolically engineered rice: a long-term study in rats. MOLECULAR NUTRITION & FOOD RESEARCH, 59(3), 490–500.
Vancouver
1.
Kiekens F, Blancquaert D, Devisscher L, Van Daele J, Stove V, Delanghe J, et al. Folates from metabolically engineered rice: a long-term study in rats. MOLECULAR NUTRITION & FOOD RESEARCH. 2015;59(3):490–500.
MLA
Kiekens, Filip, Dieter Blancquaert, Lindsey Devisscher, et al. “Folates from Metabolically Engineered Rice: a Long-term Study in Rats.” MOLECULAR NUTRITION & FOOD RESEARCH 59.3 (2015): 490–500. Print.
@article{6831308,
  abstract     = {Scope: The biological impact of folates from folate rice, a metabolically engineered (biofortified) rice line, rich in folates, was investigated. Its consumption may be helpful to fight folate deficiency. Our objective was to investigate the potential of folate rice to supply the organism with folates and evaluate its biological effectiveness using a rat model. 
Methods and results: Five groups of 12 Wistar rats were monitored during a 7/12-wk depletion/repletion trial. Animals receiving folate-free diet (0 mu g/rat/day) and those additionally receiving wild-type rice (on average 0.11 mu g/rat/day) suffered from decreased hematocrit and lower folate concentrations in both plasma and RBCs. This resulted in serious morbidity and even lethality during the trial. In contrast, all animals receiving a daily supplement of folate rice or folic acid fortified rice (on average 3.00 mu g/rat/day and 3.12 mu g/rat/day, respectively) and those receiving a positive control diet (11.4 to 25.0 mu g/rat/day), survived. In these groups, the hematocrit normalized, plasma and RBC folate concentrations increased and pronounced hyperhomocysteinemia was countered. 
Conclusion: Using an animal model, we demonstrated that biofortified folate rice is a valuable source of dietary folate, as evidenced by folate determination in plasma and RBCs, the alleviation of anemia and counteraction of pronounced hyperhomocysteinemia.},
  author       = {Kiekens, Filip and Blancquaert, Dieter and Devisscher, Lindsey and Van Daele, Jeroen and Stove, Veronique and Delanghe, Joris and Van Der Straeten, Dominique and Lambert, Willy and Stove, Christophe},
  issn         = {1613-4125},
  journal      = {MOLECULAR NUTRITION \& FOOD RESEARCH},
  keyword      = {INTESTINAL-ABSORPTION,Bioactivity,PLASMA HOMOCYSTEINE,CHEMISTRY ANALYZER,BIOAVAILABILITY,FOOD,FORTIFICATION,IMPACT,CARDIOVASCULAR-DISEASE,Folate,Biofortification,BIOFORTIFIED RICE,Rice,FOLIC-ACID,Murine model},
  language     = {eng},
  number       = {3},
  pages        = {490--500},
  title        = {Folates from metabolically engineered rice: a long-term study in rats},
  url          = {http://dx.doi.org/10.1002/mnfr.201400590},
  volume       = {59},
  year         = {2015},
}

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