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Combined adenovirus vector and hepatitis C virus envelope protein prime-boost regimen elicits T cell and neutralizing antibody immune responses

(2014) JOURNAL OF VIROLOGY. 88(10). p.5502-5510
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Abstract
Despite the recent progress in the development of new antiviral agents, hepatitis C virus (HCV) infection remains a major global health problem, and there is a need for a preventive vaccine. We previously reported that adenoviral vectors expressing HCV nonstructural proteins elicit protective T cell responses in chimpanzees and were immunogenic in healthy volunteers. Furthermore, recombinant HCV E1E2 protein formulated with adjuvant MF59 induced protective antibody responses in chimpanzees and was immunogenic in humans. To develop an HCV vaccine capable of inducing both T cell and antibody responses, we constructed adenoviral vectors expressing full-length and truncated E1E2 envelope glycoproteins from HCV genotype 1b. Heterologous prime-boost immunization regimens with adenovirus and recombinant E1E2 glycoprotein (genotype 1a) plus MF59 were evaluated in mice and guinea pigs. Adenovirus prime and protein boost induced broad HCV-specific CD8(+) and CD4(+) T cell responses and functional Th1-type IgG responses. Immune sera neutralized luciferase reporter pseudoparticles expressing HCV envelope glycoproteins (HCVpp) and a diverse panel of recombinant cell culture-derived HCV (HCVcc) strains and limited cell-to- cell HCV transmission. This study demonstrated that combining adenovirus vector with protein antigen can induce strong antibody and T cell responses that surpass immune responses achieved by either vaccine alone.
Keywords
GLYCOPROTEINS, EPIDEMIOLOGY, IMMUNIZATION, HCV, E2, IMMUNOGENICITY, TETRACYCLINE RESISTANCE, VACCINE VECTORS, DNA VACCINE, INFECTION

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Citation

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MLA
Chmielewska, Alicja M et al. “Combined Adenovirus Vector and Hepatitis C Virus Envelope Protein Prime-boost Regimen Elicits T Cell and Neutralizing Antibody Immune Responses.” JOURNAL OF VIROLOGY 88.10 (2014): 5502–5510. Print.
APA
Chmielewska, A. M., Naddeo, M., Capone, S., Ammendola, V., Hu, K., Meredith, L., Verhoye, L., et al. (2014). Combined adenovirus vector and hepatitis C virus envelope protein prime-boost regimen elicits T cell and neutralizing antibody immune responses. JOURNAL OF VIROLOGY, 88(10), 5502–5510.
Chicago author-date
Chmielewska, Alicja M, Mariarosaria Naddeo, Stefania Capone, Virginia Ammendola, Ke Hu, Luke Meredith, Lieven Verhoye, et al. 2014. “Combined Adenovirus Vector and Hepatitis C Virus Envelope Protein Prime-boost Regimen Elicits T Cell and Neutralizing Antibody Immune Responses.” Journal of Virology 88 (10): 5502–5510.
Chicago author-date (all authors)
Chmielewska, Alicja M, Mariarosaria Naddeo, Stefania Capone, Virginia Ammendola, Ke Hu, Luke Meredith, Lieven Verhoye, Malgorzata Rychlowska, Rino Rappuoli, Jeffrey B Ulmer, Stefano Colloca, Alfredo Nicosia, Riccardo Cortese, Geert Leroux-Roels, Peter Balfe, Krystyna Bienkowska-Szewczyk, Philip Meuleman, Jane A McKeating, and Antonella Folgori. 2014. “Combined Adenovirus Vector and Hepatitis C Virus Envelope Protein Prime-boost Regimen Elicits T Cell and Neutralizing Antibody Immune Responses.” Journal of Virology 88 (10): 5502–5510.
Vancouver
1.
Chmielewska AM, Naddeo M, Capone S, Ammendola V, Hu K, Meredith L, et al. Combined adenovirus vector and hepatitis C virus envelope protein prime-boost regimen elicits T cell and neutralizing antibody immune responses. JOURNAL OF VIROLOGY. 2014;88(10):5502–10.
IEEE
[1]
A. M. Chmielewska et al., “Combined adenovirus vector and hepatitis C virus envelope protein prime-boost regimen elicits T cell and neutralizing antibody immune responses,” JOURNAL OF VIROLOGY, vol. 88, no. 10, pp. 5502–5510, 2014.
@article{6830661,
  abstract     = {Despite the recent progress in the development of new antiviral agents, hepatitis C virus (HCV) infection remains a major global health problem, and there is a need for a preventive vaccine. We previously reported that adenoviral vectors expressing HCV nonstructural proteins elicit protective T cell responses in chimpanzees and were immunogenic in healthy volunteers. Furthermore, recombinant HCV E1E2 protein formulated with adjuvant MF59 induced protective antibody responses in chimpanzees and was immunogenic in humans. To develop an HCV vaccine capable of inducing both T cell and antibody responses, we constructed adenoviral vectors expressing full-length and truncated E1E2 envelope glycoproteins from HCV genotype 1b. Heterologous prime-boost immunization regimens with adenovirus and recombinant E1E2 glycoprotein (genotype 1a) plus MF59 were evaluated in mice and guinea pigs. Adenovirus prime and protein boost induced broad HCV-specific CD8(+) and CD4(+) T cell responses and functional Th1-type IgG responses. Immune sera neutralized luciferase reporter pseudoparticles expressing HCV envelope glycoproteins (HCVpp) and a diverse panel of recombinant cell culture-derived HCV (HCVcc) strains and limited cell-to- cell HCV transmission. This study demonstrated that combining adenovirus vector with protein antigen can induce strong antibody and T cell responses that surpass immune responses achieved by either vaccine alone.},
  author       = {Chmielewska, Alicja M and Naddeo, Mariarosaria and Capone, Stefania and Ammendola, Virginia and Hu, Ke and Meredith, Luke and Verhoye, Lieven and Rychlowska, Malgorzata and Rappuoli, Rino and Ulmer, Jeffrey B and Colloca, Stefano and Nicosia, Alfredo and Cortese, Riccardo and Leroux-Roels, Geert and Balfe, Peter and Bienkowska-Szewczyk, Krystyna and Meuleman, Philip and McKeating, Jane A and Folgori, Antonella},
  issn         = {0022-538X},
  journal      = {JOURNAL OF VIROLOGY},
  keywords     = {GLYCOPROTEINS,EPIDEMIOLOGY,IMMUNIZATION,HCV,E2,IMMUNOGENICITY,TETRACYCLINE RESISTANCE,VACCINE VECTORS,DNA VACCINE,INFECTION},
  language     = {eng},
  number       = {10},
  pages        = {5502--5510},
  title        = {Combined adenovirus vector and hepatitis C virus envelope protein prime-boost regimen elicits T cell and neutralizing antibody immune responses},
  url          = {http://dx.doi.org/10.1128/JVI.03574-13},
  volume       = {88},
  year         = {2014},
}

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