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GROWTH REGULATING FACTOR5 stimulates Arabidopsis chloroplast division, photosynthesis, and leaf longevity

(2015) PLANT PHYSIOLOGY. 167(3). p.817-832
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Arabidopsis (Arabidopsis thaliana) leaf development relies on subsequent phases of cell proliferation and cell expansion. During the proliferation phase, chloroplasts need to divide extensively, and during the transition from cell proliferation to expansion, they differentiate into photosynthetically active chloroplasts, providing the plant with energy. The transcription factor GROWTH REGULATING FACTOR5 (GRF5) promotes the duration of the cell proliferation period during leaf development. Here, it is shown that GRF5 also stimulates chloroplast division, resulting in a higher chloroplast number per cell with a concomitant increase in chlorophyll levels in 35S: GRF5 leaves, which can sustain higher rates of photosynthesis. Moreover, 35S: GRF5 plants show delayed leaf senescence and are more tolerant for growth on nitrogen-depleted medium. Cytokinins also stimulate leaf growth in part by extending the cell proliferation phase, simultaneously delaying the onset of the cell expansion phase. In addition, cytokinins are known to be involved in chloroplast development, nitrogen signaling, and senescence. Evidence is provided that GRF5 and cytokinins synergistically enhance cell division and chlorophyll retention after dark-induced senescence, which suggests that they also cooperate to stimulate chloroplast division and nitrogen assimilation. Taken together with the increased leaf size, ectopic expression of GRF5 has great potential to improve plant productivity.
Keywords
ABSCISIC-ACID, NEGATIVE REGULATOR, CHLOROPHYLL BIOSYNTHESIS, CELL-PROLIFERATION, PROTOCHLOROPHYLLIDE OXIDOREDUCTASE, TRANSCRIPTION FACTORS, PORC DOUBLE MUTANT, CYTOKININ SIGNAL-TRANSDUCTION, B RESPONSE REGULATORS, PROTEIN INTERACTIONS

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Citation

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Chicago
Vercruyssen, Liesbeth, Vanesa Tognetti, Nathalie Gonzalez Sanchez, Judith Van Dingenen, Liesbeth De Milde, Agnieszka Bielach, Riet De Rycke, Frank Van Breusegem, and Dirk Inzé. 2015. “GROWTH REGULATING FACTOR5 Stimulates Arabidopsis Chloroplast Division, Photosynthesis, and Leaf Longevity.” Plant Physiology 167 (3): 817–832.
APA
Vercruyssen, L., Tognetti, V., Gonzalez Sanchez, N., Van Dingenen, J., De Milde, L., Bielach, A., De Rycke, R., et al. (2015). GROWTH REGULATING FACTOR5 stimulates Arabidopsis chloroplast division, photosynthesis, and leaf longevity. PLANT PHYSIOLOGY, 167(3), 817–832.
Vancouver
1.
Vercruyssen L, Tognetti V, Gonzalez Sanchez N, Van Dingenen J, De Milde L, Bielach A, et al. GROWTH REGULATING FACTOR5 stimulates Arabidopsis chloroplast division, photosynthesis, and leaf longevity. PLANT PHYSIOLOGY. 2015;167(3):817–32.
MLA
Vercruyssen, Liesbeth, Vanesa Tognetti, Nathalie Gonzalez Sanchez, et al. “GROWTH REGULATING FACTOR5 Stimulates Arabidopsis Chloroplast Division, Photosynthesis, and Leaf Longevity.” PLANT PHYSIOLOGY 167.3 (2015): 817–832. Print.
@article{6829864,
  abstract     = {Arabidopsis (Arabidopsis thaliana) leaf development relies on subsequent phases of cell proliferation and cell expansion. During the proliferation phase, chloroplasts need to divide extensively, and during the transition from cell proliferation to expansion, they differentiate into photosynthetically active chloroplasts, providing the plant with energy. The transcription factor GROWTH REGULATING FACTOR5 (GRF5) promotes the duration of the cell proliferation period during leaf development. Here, it is shown that GRF5 also stimulates chloroplast division, resulting in a higher chloroplast number per cell with a concomitant increase in chlorophyll levels in 35S: GRF5 leaves, which can sustain higher rates of photosynthesis. Moreover, 35S: GRF5 plants show delayed leaf senescence and are more tolerant for growth on nitrogen-depleted medium. Cytokinins also stimulate leaf growth in part by extending the cell proliferation phase, simultaneously delaying the onset of the cell expansion phase. In addition, cytokinins are known to be involved in chloroplast development, nitrogen signaling, and senescence. Evidence is provided that GRF5 and cytokinins synergistically enhance cell division and chlorophyll retention after dark-induced senescence, which suggests that they also cooperate to stimulate chloroplast division and nitrogen assimilation. Taken together with the increased leaf size, ectopic expression of GRF5 has great potential to improve plant productivity.},
  author       = {Vercruyssen, Liesbeth and Tognetti, Vanesa and Gonzalez Sanchez, Nathalie and Van Dingenen, Judith and De Milde, Liesbeth and Bielach, Agnieszka and De Rycke, Riet and Van Breusegem, Frank and Inz{\'e}, Dirk},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ABSCISIC-ACID,NEGATIVE REGULATOR,CHLOROPHYLL BIOSYNTHESIS,CELL-PROLIFERATION,PROTOCHLOROPHYLLIDE OXIDOREDUCTASE,TRANSCRIPTION FACTORS,PORC DOUBLE MUTANT,CYTOKININ SIGNAL-TRANSDUCTION,B RESPONSE REGULATORS,PROTEIN INTERACTIONS},
  language     = {eng},
  number       = {3},
  pages        = {817--832},
  title        = {GROWTH REGULATING FACTOR5 stimulates Arabidopsis chloroplast division, photosynthesis, and leaf longevity},
  url          = {http://dx.doi.org/10.1104/pp.114.256180},
  volume       = {167},
  year         = {2015},
}

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