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ARACINs, Brassicaceae-specific peptides exhibiting antifungal activities against necrotrophic pathogens in Arabidopsis

(2015) PLANT PHYSIOLOGY. 167(3). p.1017-1029
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Abstract
Plants have developed a variety of mechanisms to cope with abiotic and biotic stresses. In a previous subcellular localization study of hydrogen peroxide-responsive proteins, two peptides with an unknown function (designated ARACIN1 and ARACIN2) have been identified. These peptides are structurally very similar but are transcriptionally differentially regulated during abiotic stresses during Botrytis cinerea infection or after benzothiadiazole and methyl jasmonate treatments. In Arabidopsis (Arabidopsis thaliana), these paralogous genes are positioned in tandem within a cluster of pathogen defense-related genes. Both ARACINs are small, cationic, and hydrophobic peptides, known characteristics for antimicrobial peptides. Their genes are expressed in peripheral cell layers prone to pathogen entry and are lineage specific to the Brassicaceae family. In vitro bioassays demonstrated that both ARACIN peptides have a direct antifungal effect against the agronomically and economically important necrotrophic fungi B. cinerea, Alternaria brassicicola, Fusarium graminearum, and Sclerotinia sclerotiorum and yeast (Saccharomyces cerevisiae). In addition, transgenic Arabidopsis plants that ectopically express ARACIN1 are protected better against infections with both B. cinerea and A. brassicicola. Therefore, we can conclude that both ARACINs act as antimicrobial peptides.
Keywords
HOST-DEFENSE, THALIANA TRANSCRIPTOME, ALTERNARIA-BRASSICICOLA, SIGNAL-TRANSDUCTION, GENE-EXPRESSION, PLANT DEFENSINS, PROTEASE INHIBITORS, FEMALE GAMETOPHYTE, DISEASE RESISTANCE, ANTIMICROBIAL PEPTIDES

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MLA
Neukermans, Jenny et al. “ARACINs, Brassicaceae-specific Peptides Exhibiting Antifungal Activities Against Necrotrophic Pathogens in Arabidopsis.” PLANT PHYSIOLOGY 167.3 (2015): 1017–1029. Print.
APA
Neukermans, J., Inzé, A., Mathys, J., De Coninck, B., Van De Cotte, B., Cammue, B. P., & Van Breusegem, F. (2015). ARACINs, Brassicaceae-specific peptides exhibiting antifungal activities against necrotrophic pathogens in Arabidopsis. PLANT PHYSIOLOGY, 167(3), 1017–1029.
Chicago author-date
Neukermans, Jenny, Annelies Inzé, Janick Mathys, Barbara De Coninck, Brigitte Van De Cotte, Bruno PA Cammue, and Frank Van Breusegem. 2015. “ARACINs, Brassicaceae-specific Peptides Exhibiting Antifungal Activities Against Necrotrophic Pathogens in Arabidopsis.” Plant Physiology 167 (3): 1017–1029.
Chicago author-date (all authors)
Neukermans, Jenny, Annelies Inzé, Janick Mathys, Barbara De Coninck, Brigitte Van De Cotte, Bruno PA Cammue, and Frank Van Breusegem. 2015. “ARACINs, Brassicaceae-specific Peptides Exhibiting Antifungal Activities Against Necrotrophic Pathogens in Arabidopsis.” Plant Physiology 167 (3): 1017–1029.
Vancouver
1.
Neukermans J, Inzé A, Mathys J, De Coninck B, Van De Cotte B, Cammue BP, et al. ARACINs, Brassicaceae-specific peptides exhibiting antifungal activities against necrotrophic pathogens in Arabidopsis. PLANT PHYSIOLOGY. 2015;167(3):1017–29.
IEEE
[1]
J. Neukermans et al., “ARACINs, Brassicaceae-specific peptides exhibiting antifungal activities against necrotrophic pathogens in Arabidopsis,” PLANT PHYSIOLOGY, vol. 167, no. 3, pp. 1017–1029, 2015.
@article{6826230,
  abstract     = {{Plants have developed a variety of mechanisms to cope with abiotic and biotic stresses. In a previous subcellular localization study of hydrogen peroxide-responsive proteins, two peptides with an unknown function (designated ARACIN1 and ARACIN2) have been identified. These peptides are structurally very similar but are transcriptionally differentially regulated during abiotic stresses during Botrytis cinerea infection or after benzothiadiazole and methyl jasmonate treatments. In Arabidopsis (Arabidopsis thaliana), these paralogous genes are positioned in tandem within a cluster of pathogen defense-related genes. Both ARACINs are small, cationic, and hydrophobic peptides, known characteristics for antimicrobial peptides. Their genes are expressed in peripheral cell layers prone to pathogen entry and are lineage specific to the Brassicaceae family. In vitro bioassays demonstrated that both ARACIN peptides have a direct antifungal effect against the agronomically and economically important necrotrophic fungi B. cinerea, Alternaria brassicicola, Fusarium graminearum, and Sclerotinia sclerotiorum and yeast (Saccharomyces cerevisiae). In addition, transgenic Arabidopsis plants that ectopically express ARACIN1 are protected better against infections with both B. cinerea and A. brassicicola. Therefore, we can conclude that both ARACINs act as antimicrobial peptides.}},
  author       = {{Neukermans, Jenny and Inzé, Annelies and Mathys, Janick and De Coninck, Barbara and Van De Cotte, Brigitte and Cammue, Bruno PA and Van Breusegem, Frank}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{HOST-DEFENSE,THALIANA TRANSCRIPTOME,ALTERNARIA-BRASSICICOLA,SIGNAL-TRANSDUCTION,GENE-EXPRESSION,PLANT DEFENSINS,PROTEASE INHIBITORS,FEMALE GAMETOPHYTE,DISEASE RESISTANCE,ANTIMICROBIAL PEPTIDES}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1017--1029}},
  title        = {{ARACINs, Brassicaceae-specific peptides exhibiting antifungal activities against necrotrophic pathogens in Arabidopsis}},
  url          = {{http://dx.doi.org/10.1104/pp.114.255505}},
  volume       = {{167}},
  year         = {{2015}},
}

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