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Spodoptera littoralis-induced lectin expression in tobacco

(2009) PLANT AND CELL PHYSIOLOGY. 50(6). p.1142-1155
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Abstract
The induced defense response in plants towards herbivores is mainly regulated by jasmonates and leads to the accumulation of so-called jasmonate-induced proteins. Recently, a jasmonate (JA) inducible lectin called Nicotiana tabacum agglutinin or NICTABA was discovered in tobacco (N. tabacum cv Samsun) leaves. Tobacco plants also accumulate the lectin after insect attack by caterpillars. To study the functional role of NICTABA, the accumulation of the JA precursor 12-oxophytodienoic acid (OPDA), JA as well as different JA metabolites were analyzed in tobacco leaves after herbivory by larvae of the cotton leafworm (Spodoptera littoralis) and correlated with NICTABA accumulation. It was shown that OPDA, JA as well as its methyl ester can trigger NICTABA accumulation. However, hydroxylation of JA and its subsequent sulfation and glucosylation results in inactive compounds that have lost the capacity to induce NICTABA gene expression. The expression profile of NICTABA after caterpillar feeding was recorded in local as well as in systemic leaves, and compared to the expression of several genes encoding defense proteins, and genes encoding a tobacco systemin and the allene oxide cyclase, an enzyme in JA biosynthesis. Furthermore, the accumulation of NICTABA was quantified after S. littoralis herbivory and immunofluorescence microscopy was used to study the localization of NICTABA in the tobacco leaf.
Keywords
12-OXO-PHYTODIENOIC ACID, HERBIVORE RESISTANCE, GENE-EXPRESSION, WOUND RESPONSE, NICOTIANA-ATTENUATA, JASMONIC ACID, ALLENE OXIDE CYCLASE, Nictaba, Nicotiana tabacum, Plant insect interaction, Lectin, Agglutinin, Carbohydrate binding, INHIBITOR PRODUCTION, OXYLIPIN SIGNATURE, DEFENSE RESPONSES

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Citation

Please use this url to cite or link to this publication:

MLA
Vandenborre, Gianni et al. “Spodoptera Littoralis-induced Lectin Expression in Tobacco.” PLANT AND CELL PHYSIOLOGY 50.6 (2009): 1142–1155. Print.
APA
Vandenborre, G., Miersch, O., Hause, B., Smagghe, G., Wasternack, C., & Van Damme, E. (2009). Spodoptera littoralis-induced lectin expression in tobacco. PLANT AND CELL PHYSIOLOGY, 50(6), 1142–1155.
Chicago author-date
Vandenborre, Gianni, Otto Miersch, Bettina Hause, Guy Smagghe, Claus Wasternack, and Els Van Damme. 2009. “Spodoptera Littoralis-induced Lectin Expression in Tobacco.” Plant and Cell Physiology 50 (6): 1142–1155.
Chicago author-date (all authors)
Vandenborre, Gianni, Otto Miersch, Bettina Hause, Guy Smagghe, Claus Wasternack, and Els Van Damme. 2009. “Spodoptera Littoralis-induced Lectin Expression in Tobacco.” Plant and Cell Physiology 50 (6): 1142–1155.
Vancouver
1.
Vandenborre G, Miersch O, Hause B, Smagghe G, Wasternack C, Van Damme E. Spodoptera littoralis-induced lectin expression in tobacco. PLANT AND CELL PHYSIOLOGY. 2009;50(6):1142–55.
IEEE
[1]
G. Vandenborre, O. Miersch, B. Hause, G. Smagghe, C. Wasternack, and E. Van Damme, “Spodoptera littoralis-induced lectin expression in tobacco,” PLANT AND CELL PHYSIOLOGY, vol. 50, no. 6, pp. 1142–1155, 2009.
@article{681522,
  abstract     = {The induced defense response in plants towards herbivores is mainly regulated by jasmonates and leads to the accumulation of so-called jasmonate-induced proteins. Recently, a jasmonate (JA) inducible lectin called Nicotiana tabacum agglutinin or NICTABA was discovered in tobacco (N. tabacum cv Samsun) leaves. Tobacco plants also accumulate the lectin after insect attack by caterpillars. To study the functional role of NICTABA, the accumulation of the JA precursor 12-oxophytodienoic acid (OPDA), JA as well as different JA metabolites were analyzed in tobacco leaves after herbivory by larvae of the cotton leafworm (Spodoptera littoralis) and correlated with NICTABA accumulation. It was shown that OPDA, JA as well as its methyl ester can trigger NICTABA accumulation. However, hydroxylation of JA and its subsequent sulfation and glucosylation results in inactive compounds that have lost the capacity to induce NICTABA gene expression. The expression profile of NICTABA after caterpillar feeding was recorded in local as well as in systemic leaves, and compared to the expression of several genes encoding defense proteins, and genes encoding a tobacco systemin and the allene oxide cyclase, an enzyme in JA biosynthesis. Furthermore, the accumulation of NICTABA was quantified after S. littoralis herbivory and immunofluorescence microscopy was used to study the localization of NICTABA in the tobacco leaf.},
  author       = {Vandenborre, Gianni and Miersch, Otto and Hause, Bettina and Smagghe, Guy and Wasternack, Claus and Van Damme, Els},
  issn         = {0032-0781},
  journal      = {PLANT AND CELL PHYSIOLOGY},
  keywords     = {12-OXO-PHYTODIENOIC ACID,HERBIVORE RESISTANCE,GENE-EXPRESSION,WOUND RESPONSE,NICOTIANA-ATTENUATA,JASMONIC ACID,ALLENE OXIDE CYCLASE,Nictaba,Nicotiana tabacum,Plant insect interaction,Lectin,Agglutinin,Carbohydrate binding,INHIBITOR PRODUCTION,OXYLIPIN SIGNATURE,DEFENSE RESPONSES},
  language     = {eng},
  number       = {6},
  pages        = {1142--1155},
  title        = {Spodoptera littoralis-induced lectin expression in tobacco},
  url          = {http://dx.doi.org/10.1093/pcp/pcp065},
  volume       = {50},
  year         = {2009},
}

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