Ghent University Academic Bibliography

Advanced

Study of the gastrointestinal biotransformation of zearalenone in a Caco-2 cell culture system with liquid chromatographic methods

Annelore Schaut, Sarah De Saeger UGent, T. Sergent, Yves Schneider, Yvan Larondelle, Luc Pussemier and Carlos Van Peteghem UGent (2008) JOURNAL OF APPLIED TOXICOLOGY. 28(8). p.966-973
abstract
A high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) was developed and validated for the detection of zearalenone (ZON), alpha-zearalenon (alpha-ZOL) and beta-zearalenol (beta-ZOL) in in vitro biological samples. Furthermore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the detection of ZON, alpha-ZOL, beta-ZOL, alpha-zearalanol (alpha-ZAL) and beta-zearalanol (beta-ZAL) in in vitro biological samples. Zearalanone (ZAN) was used as internal standard in both methods. The limit of detection/limit of quantitation (LOD/LOQ) values of ZON, alpha-ZOL and beta-ZOL were 2/7, 2/7 and 4/13 mu g 1(-1), respectively, for the HPLC-FLD method. For the LC-MS/MS method LOD/LOQ values for ZON, alpha-ZOL, beta-ZOL, alpha-ZAL and beta-ZAL were 6/20, 5/17, 4/14, 9/30 and 6/19 mu g 1(-1), respectively. Within-day and between-day precision were less then 11 and 14%, respectively for the HPLC-FLD method, and both less then 20% for the LC-MS/MS method. The recovery of ZON and its metabolities ranged between 73 and 89% for the HPLC-FLD method and between 69 and 112% for the LC-MS/MS method. The methods were used for the detection of the compounds in in vitro biological samples, obtained with human intestinal Caco-2 cells culture experiments. The 8-days post-confluent Caco-2 cells were treated with ZON or a mixture of ZON and imazalil (IMA). After an incubation time of 24 h the samples were analyzed with the HPLC-FLD method. Neither ZON nor its derivatives were detected in the samples. The disappearance of ZON could possibly point out the formation of phase II metabolities like glucuronide conjugates. There, samples were pretreated with beta-glucuronidase before LC-MS/MS analysis. The LC-MS/MS results showed that ZON, alpha-ZOL and beta-ZOL could only be detected in the beta-glucuronidase pretreated samples. This confirmed the formation of glucuronide conjugates and the hydroxylation of ZON during the incubation with Caco-2 cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
analytical methodology, biotransformation, zearalenone, imazalil, Caco-2, glucuronidation
journal title
JOURNAL OF APPLIED TOXICOLOGY
J. Appl. Toxicol.
editor
Marianne Bailleul UGent
volume
28
issue
8
pages
966 - 973
Web of Science type
Article
Web of Science id
000261114100005
JCR category
TOXICOLOGY
JCR impact factor
2.127 (2008)
JCR rank
42/74 (2008)
JCR quartile
3 (2008)
ISSN
0260-437X
DOI
10.1002/jat.1362
language
English
UGent publication?
yes
classification
A1
id
680923
handle
http://hdl.handle.net/1854/LU-680923
date created
2009-06-05 16:05:36
date last changed
2009-06-08 10:23:34
@article{680923,
  abstract     = {A high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) was developed and validated for the detection of zearalenone (ZON), alpha-zearalenon (alpha-ZOL) and beta-zearalenol (beta-ZOL) in in vitro biological samples. Furthermore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the detection of ZON, alpha-ZOL, beta-ZOL, alpha-zearalanol (alpha-ZAL) and beta-zearalanol (beta-ZAL) in in vitro biological samples. Zearalanone (ZAN) was used as internal standard in both methods. The limit of detection/limit of quantitation (LOD/LOQ) values of ZON, alpha-ZOL and beta-ZOL were 2/7, 2/7 and 4/13 mu g 1(-1), respectively, for the HPLC-FLD method. For the LC-MS/MS method LOD/LOQ values for ZON, alpha-ZOL, beta-ZOL, alpha-ZAL and beta-ZAL were 6/20, 5/17, 4/14, 9/30 and 6/19 mu g 1(-1), respectively. Within-day and between-day precision were less then 11 and 14\%, respectively for the HPLC-FLD method, and both less then 20\% for the LC-MS/MS method. The recovery of ZON and its metabolities ranged between 73 and 89\% for the HPLC-FLD method and between 69 and 112\% for the LC-MS/MS method. The methods were used for the detection of the compounds in in vitro biological samples, obtained with human intestinal Caco-2 cells culture experiments. The 8-days post-confluent Caco-2 cells were treated with ZON or a mixture of ZON and imazalil (IMA). After an incubation time of 24 h the samples were analyzed with the HPLC-FLD method. Neither ZON nor its derivatives were detected in the samples. The disappearance of ZON could possibly point out the formation of phase II metabolities like glucuronide conjugates. There, samples were pretreated with beta-glucuronidase before LC-MS/MS analysis. The LC-MS/MS results showed that ZON, alpha-ZOL and beta-ZOL could only be detected in the beta-glucuronidase pretreated samples. This confirmed the formation of glucuronide conjugates and the hydroxylation of ZON during the incubation with Caco-2 cells.},
  author       = {Schaut, Annelore and De Saeger, Sarah and Sergent, T. and Schneider, Yves and Larondelle, Yvan and Pussemier, Luc and Van Peteghem, Carlos},
  editor       = {Bailleul, Marianne},
  issn         = {0260-437X},
  journal      = {JOURNAL OF APPLIED TOXICOLOGY},
  keyword      = {analytical methodology,biotransformation,zearalenone,imazalil,Caco-2,glucuronidation},
  language     = {eng},
  number       = {8},
  pages        = {966--973},
  title        = {Study of the gastrointestinal biotransformation of zearalenone in a Caco-2 cell culture system with liquid chromatographic methods},
  url          = {http://dx.doi.org/10.1002/jat.1362},
  volume       = {28},
  year         = {2008},
}

Chicago
Schaut, Annelore, Sarah De Saeger, T. Sergent, Yves Schneider, Yvan Larondelle, Luc Pussemier, and Carlos Van Peteghem. 2008. “Study of the Gastrointestinal Biotransformation of Zearalenone in a Caco-2 Cell Culture System with Liquid Chromatographic Methods.” Ed. Marianne Bailleul. Journal of Applied Toxicology 28 (8): 966–973.
APA
Schaut, A., De Saeger, S., Sergent, T., Schneider, Y., Larondelle, Y., Pussemier, L., & Van Peteghem, C. (2008). Study of the gastrointestinal biotransformation of zearalenone in a Caco-2 cell culture system with liquid chromatographic methods. (M. Bailleul, Ed.)JOURNAL OF APPLIED TOXICOLOGY, 28(8), 966–973.
Vancouver
1.
Schaut A, De Saeger S, Sergent T, Schneider Y, Larondelle Y, Pussemier L, et al. Study of the gastrointestinal biotransformation of zearalenone in a Caco-2 cell culture system with liquid chromatographic methods. Bailleul M, editor. JOURNAL OF APPLIED TOXICOLOGY. 2008;28(8):966–73.
MLA
Schaut, Annelore, Sarah De Saeger, T. Sergent, et al. “Study of the Gastrointestinal Biotransformation of Zearalenone in a Caco-2 Cell Culture System with Liquid Chromatographic Methods.” Ed. Marianne Bailleul. JOURNAL OF APPLIED TOXICOLOGY 28.8 (2008): 966–973. Print.