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In vitro fertilizing capacity of frozen-thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation

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Abstract
Contents Barriers to the use of density gradient centrifugation for preparing animal spermatozoa for artificial insemination (AI) include the scarcity of animal-specific formulations and the daunting prospect of processing large volumes of ejaculate in small aliquots (1.5 ml extended semen). Recently, new colloid formulations have been tested in vitro in a modified procedure, centrifugation on a single layer of colloid. The present study investigated the fertilizing ability during in vitro fertilization (IVF) of frozen-thawed bovine spermatozoa following centrifugation through a single layer of glycerolpropylsilane (GS)-coated silica colloid with a species-specific formulation (patent applied for; treatment, T). Controls (C) included centrifugation through gradients of either the same colloid (C1) or Percolltrade mark (C2). Sperm recovery surpassed 50% for both C1-C2 and T (n.s.). Mean values of various parameters of computerized analysis of sperm motility did not differ between T and C1 (n.s.), and only the proportions of path straightness and linearity were lower in T vs C2 (p < 0.05). In T, the mean (+/-SD) percentages of fertilization rate, blastocyst development rate and the total number of blastomeres were 58.1 +/- 23.3%, 24.5 +/- 14.3% and 94.6 +/- 23.4%, respectively. The proportions did not differ significantly from controls (C1/C2). Therefore, centrifugation through a single layer of colloid offers an alternative method to density gradient centrifugation for selection of viable, potentially fertile frozen-thawed bull spermatozoa. This single-layer technique is gentle, versatile and convenient because it facilitates scaling-up the process of sperm preparation to allow larger numbers of spermatozoa (for instance, whole ejaculates) to be processed for AI.
Keywords
SEMEN, SPERM, PARAMETERS, PERCOLL(R), QUALITY

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MLA
Thys, Mirjan, et al. “In Vitro Fertilizing Capacity of Frozen-Thawed Bull Spermatozoa Selected by Single-Layer (Glycidoxypropyltrimethoxysilane) Silane-Coated Silica Colloidal Centrifugation.” REPRODUCTION IN DOMESTIC ANIMALS, vol. 44, no. 3, 2009, pp. 390–94, doi:10.1111/j.1439-0531.2008.01081.x.
APA
Thys, M., Vandaele, L., Morrell, J. M., Mestach, J., Van Soom, A., Hoogewijs, M., & Rodriguez-Martinez, H. (2009). In vitro fertilizing capacity of frozen-thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation. REPRODUCTION IN DOMESTIC ANIMALS, 44(3), 390–394. https://doi.org/10.1111/j.1439-0531.2008.01081.x
Chicago author-date
Thys, Mirjan, Leen Vandaele, Jane M Morrell, Johanna Mestach, Ann Van Soom, Maarten Hoogewijs, and Heriberto Rodriguez-Martinez. 2009. “In Vitro Fertilizing Capacity of Frozen-Thawed Bull Spermatozoa Selected by Single-Layer (Glycidoxypropyltrimethoxysilane) Silane-Coated Silica Colloidal Centrifugation.” REPRODUCTION IN DOMESTIC ANIMALS 44 (3): 390–94. https://doi.org/10.1111/j.1439-0531.2008.01081.x.
Chicago author-date (all authors)
Thys, Mirjan, Leen Vandaele, Jane M Morrell, Johanna Mestach, Ann Van Soom, Maarten Hoogewijs, and Heriberto Rodriguez-Martinez. 2009. “In Vitro Fertilizing Capacity of Frozen-Thawed Bull Spermatozoa Selected by Single-Layer (Glycidoxypropyltrimethoxysilane) Silane-Coated Silica Colloidal Centrifugation.” REPRODUCTION IN DOMESTIC ANIMALS 44 (3): 390–394. doi:10.1111/j.1439-0531.2008.01081.x.
Vancouver
1.
Thys M, Vandaele L, Morrell JM, Mestach J, Van Soom A, Hoogewijs M, et al. In vitro fertilizing capacity of frozen-thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation. REPRODUCTION IN DOMESTIC ANIMALS. 2009;44(3):390–4.
IEEE
[1]
M. Thys et al., “In vitro fertilizing capacity of frozen-thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation,” REPRODUCTION IN DOMESTIC ANIMALS, vol. 44, no. 3, pp. 390–394, 2009.
@article{674522,
  abstract     = {{Contents Barriers to the use of density gradient centrifugation for preparing animal spermatozoa for artificial insemination (AI) include the scarcity of animal-specific formulations and the daunting prospect of processing large volumes of ejaculate in small aliquots (1.5 ml extended semen). Recently, new colloid formulations have been tested in vitro in a modified procedure, centrifugation on a single layer of colloid. The present study investigated the fertilizing ability during in vitro fertilization (IVF) of frozen-thawed bovine spermatozoa following centrifugation through a single layer of glycerolpropylsilane (GS)-coated silica colloid with a species-specific formulation (patent applied for; treatment, T). Controls (C) included centrifugation through gradients of either the same colloid (C1) or Percolltrade mark (C2). Sperm recovery surpassed 50% for both C1-C2 and T (n.s.). Mean values of various parameters of computerized analysis of sperm motility did not differ between T and C1 (n.s.), and only the proportions of path straightness and linearity were lower in T vs C2 (p < 0.05). In T, the mean (+/-SD) percentages of fertilization rate, blastocyst development rate and the total number of blastomeres were 58.1 +/- 23.3%, 24.5 +/- 14.3% and 94.6 +/- 23.4%, respectively. The proportions did not differ significantly from controls (C1/C2). Therefore, centrifugation through a single layer of colloid offers an alternative method to density gradient centrifugation for selection of viable, potentially fertile frozen-thawed bull spermatozoa. This single-layer technique is gentle, versatile and convenient because it facilitates scaling-up the process of sperm preparation to allow larger numbers of spermatozoa (for instance, whole ejaculates) to be processed for AI.}},
  author       = {{Thys, Mirjan and Vandaele, Leen and Morrell, Jane M and Mestach, Johanna and Van Soom, Ann and Hoogewijs, Maarten and Rodriguez-Martinez, Heriberto}},
  issn         = {{0936-6768}},
  journal      = {{REPRODUCTION IN DOMESTIC ANIMALS}},
  keywords     = {{SEMEN,SPERM,PARAMETERS,PERCOLL(R),QUALITY}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{390--394}},
  title        = {{In vitro fertilizing capacity of frozen-thawed bull spermatozoa selected by single-layer (glycidoxypropyltrimethoxysilane) silane-coated silica colloidal centrifugation}},
  url          = {{http://dx.doi.org/10.1111/j.1439-0531.2008.01081.x}},
  volume       = {{44}},
  year         = {{2009}},
}

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