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The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour

Christel Moons (UGent) , Kathelijne Peremans (UGent) , Simon Vermeire (UGent) , Eva Vandermeulen (UGent) , Andre Dobbeleir, Katleen Hermans (UGent) , Frank Odberg (UGent) and Kurt Audenaert (UGent)
Author
Organization
Abstract
Functional molecular imaging is becoming increasingly popular for in vivo research on small animals, because it has a number of scientific advantages over ex vivo methods. The molecular parameters themselves can be used in other areas of investigation also, such as monitoring of the dopaminergic and serotonergic involvement in the development of stereotypic behaviour. In Single Photon Emission Computed Tomography (SPELT), a radioactive substance with specific affinity for a certain molecular target is injected intravenously and after a period of time the radioactivity that is not washed out from the region of interest, is measured. A relative measure of quantification, i.e., the binding index (BI), can then be calculated. This paper aims to introduce a broad readership to one possible application of SPELT by presenting preliminary data about the dopamine transporter (DAT) status in the Mongolian gerbil (Meriones unguiculatus). (99m)Technetium-labelled Ethylcysteinate Dimer tracer (24.42 +/- 5.92 MBq) was injected in the femoral vein of four gerbils to provide brain perfusion images that allow anatomical identification of DAT-rich regions that were imaged in another four gerbils using I-123-labelled FP-CIT tracer (44.33 +/- 11.66 MBq). Furthermore, the optimal scan time for FP-CIT was established in one gerbil. The study was successful in obtaining brain perfusion images as well as demonstrating regional binding of FP-CIT to the basal ganglia, DAT-rich areas in the brain. The optimal scan time for DAT-imaging was 4 1/2 hours. Our preliminary data suggest the Mongolian gerbil is a suitable model for combining SPELT and behavioural observations in the investigation of stereotypic behaviour.
Keywords
MICE, ANESTHESIA, PINHOLE, GERBIL MERIONES-UNGUICULATUS, SMALL ANIMAL SPECT, MONGOLIAN GERBIL, TRANSPORTER BLOCKADE, RATS, PARKINSONS-DISEASE, RECEPTOR

Citation

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MLA
Moons, Christel et al. “The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour.” SCANDINAVIAN JOURNAL OF LABORATORY ANIMAL SCIENCE 35.4 (2008): 221–229. Print.
APA
Moons, C., Peremans, K., Vermeire, S., Vandermeulen, E., Dobbeleir, A., Hermans, K., Odberg, F., et al. (2008). The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour. SCANDINAVIAN JOURNAL OF LABORATORY ANIMAL SCIENCE, 35(4), 221–229.
Chicago author-date
Moons, Christel, Kathelijne Peremans, Simon Vermeire, Eva Vandermeulen, Andre Dobbeleir, Katleen Hermans, Frank Odberg, and Kurt Audenaert. 2008. “The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour.” Scandinavian Journal of Laboratory Animal Science 35 (4): 221–229.
Chicago author-date (all authors)
Moons, Christel, Kathelijne Peremans, Simon Vermeire, Eva Vandermeulen, Andre Dobbeleir, Katleen Hermans, Frank Odberg, and Kurt Audenaert. 2008. “The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour.” Scandinavian Journal of Laboratory Animal Science 35 (4): 221–229.
Vancouver
1.
Moons C, Peremans K, Vermeire S, Vandermeulen E, Dobbeleir A, Hermans K, et al. The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour. SCANDINAVIAN JOURNAL OF LABORATORY ANIMAL SCIENCE. Denmark: SCANDINAVIAN FEDERATION LABORATORY ANIMAL SCIENCE; 2008;35(4):221–9.
IEEE
[1]
C. Moons et al., “The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour,” SCANDINAVIAN JOURNAL OF LABORATORY ANIMAL SCIENCE, vol. 35, no. 4, pp. 221–229, 2008.
@article{672418,
  abstract     = {Functional molecular imaging is becoming increasingly popular for in vivo research on small animals, because it has a number of scientific advantages over ex vivo methods. The molecular parameters themselves can be used in other areas of investigation also, such as monitoring of the dopaminergic and serotonergic involvement in the development of stereotypic behaviour. In Single Photon Emission Computed Tomography (SPELT), a radioactive substance with specific affinity for a certain molecular target is injected intravenously and after a period of time the radioactivity that is not washed out from the region of interest, is measured. A relative measure of quantification, i.e., the binding index (BI), can then be calculated. This paper aims to introduce a broad readership to one possible application of SPELT by presenting preliminary data about the dopamine transporter (DAT) status in the Mongolian gerbil (Meriones unguiculatus). (99m)Technetium-labelled Ethylcysteinate Dimer tracer (24.42 +/- 5.92 MBq) was injected in the femoral vein of four gerbils to provide brain perfusion images that allow anatomical identification of DAT-rich regions that were imaged in another four gerbils using I-123-labelled FP-CIT tracer (44.33 +/- 11.66 MBq). Furthermore, the optimal scan time for FP-CIT was established in one gerbil. The study was successful in obtaining brain perfusion images as well as demonstrating regional binding of FP-CIT to the basal ganglia, DAT-rich areas in the brain. The optimal scan time for DAT-imaging was 4 1/2 hours. Our preliminary data suggest the Mongolian gerbil is a suitable model for combining SPELT and behavioural observations in the investigation of stereotypic behaviour.},
  author       = {Moons, Christel and Peremans, Kathelijne and Vermeire, Simon and Vandermeulen, Eva and Dobbeleir, Andre and Hermans, Katleen and Odberg, Frank and Audenaert, Kurt},
  issn         = {0901-3393},
  journal      = {SCANDINAVIAN JOURNAL OF LABORATORY ANIMAL SCIENCE},
  keywords     = {MICE,ANESTHESIA,PINHOLE,GERBIL MERIONES-UNGUICULATUS,SMALL ANIMAL SPECT,MONGOLIAN GERBIL,TRANSPORTER BLOCKADE,RATS,PARKINSONS-DISEASE,RECEPTOR},
  language     = {eng},
  number       = {4},
  pages        = {221--229},
  publisher    = {SCANDINAVIAN FEDERATION LABORATORY ANIMAL SCIENCE},
  title        = {The Use of HiSPECT to Investigate Dopaminergic Involvement in the Development of Stereotypic Behaviour},
  volume       = {35},
  year         = {2008},
}

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