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Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed

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Abstract
Ergot alkaloids are mycotoxins which can be found in food based on cereal-crops, due to a contamination of plants by fungi of the genus Claviceps. The ingestion of ergot contaminated cereal crops can lead to a severe poisoning known as ergotism. For food and feed safety purposes, the extraction of ergot alkaloids from ergot contaminated flour was investigated. For the specific recognition of ergot alkaloids, DNA aptamer ligands specially selected for ergot alkaloids were grafted onto silica gel in order to construct a specific solid phase extraction system. The aptamer-functionalized silica gels were used to extract ergot alkaloids from a contaminated rye feed sample. The presence of ergot alkaloids eluted from the aptamer-functionalized silica gels was analyzed using LC-QTOF-MS. By using this simple system, it was possible to specifically extract ergosine, ergokryptine and ergocornine from an ergot contaminated rye feed sample. This aptamer-based extraction tool shows the applicability of aptamers for the specific extraction of toxins or natural compounds from turbid matrices in a one-step procedure.
Keywords
Ergokryptine, Ergocornine, Ergosine, Single-Stranded Nucleic Acid Aptamer, Aptamer-Functionalized Silica Gel

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MLA
Rouah-Martin, Elsa et al. “Aptamer-based Extraction of Ergot Alkaloids from Ergot Contaminated Rye Feed.” ADVANCES IN BIOSCIENCE AND BIOTECHNOLOGY 5.8 (2014): 692–698. Print.
APA
Rouah-Martin, E., Maho, W., Mehta, J., De Saeger, S., Covaci, A., Van Dorst, B., Blust, R., et al. (2014). Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed. ADVANCES IN BIOSCIENCE AND BIOTECHNOLOGY, 5(8), 692–698.
Chicago author-date
Rouah-Martin, Elsa, Walid Maho, Jaytry Mehta, Sarah De Saeger, Adrian Covaci, Bieke Van Dorst, Ronny Blust, and Johan Robbens. 2014. “Aptamer-based Extraction of Ergot Alkaloids from Ergot Contaminated Rye Feed.” Advances in Bioscience and Biotechnology 5 (8): 692–698.
Chicago author-date (all authors)
Rouah-Martin, Elsa, Walid Maho, Jaytry Mehta, Sarah De Saeger, Adrian Covaci, Bieke Van Dorst, Ronny Blust, and Johan Robbens. 2014. “Aptamer-based Extraction of Ergot Alkaloids from Ergot Contaminated Rye Feed.” Advances in Bioscience and Biotechnology 5 (8): 692–698.
Vancouver
1.
Rouah-Martin E, Maho W, Mehta J, De Saeger S, Covaci A, Van Dorst B, et al. Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed. ADVANCES IN BIOSCIENCE AND BIOTECHNOLOGY. 2014;5(8):692–8.
IEEE
[1]
E. Rouah-Martin et al., “Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed,” ADVANCES IN BIOSCIENCE AND BIOTECHNOLOGY, vol. 5, no. 8, pp. 692–698, 2014.
@article{6717487,
  abstract     = {Ergot alkaloids are mycotoxins which can be found in food based on cereal-crops, due to a contamination of plants by fungi of the genus Claviceps. The ingestion of ergot contaminated cereal crops can lead to a severe poisoning known as ergotism. For food and feed safety purposes, the extraction of ergot alkaloids from ergot contaminated flour was investigated. For the specific recognition of ergot alkaloids, DNA aptamer ligands specially selected for ergot alkaloids were grafted onto silica gel in order to construct a specific solid phase extraction system. The aptamer-functionalized silica gels were used to extract ergot alkaloids from a contaminated rye feed sample. The presence of ergot alkaloids eluted from the aptamer-functionalized silica gels was analyzed using LC-QTOF-MS. By using this simple system, it was possible to specifically extract ergosine, ergokryptine and ergocornine from an ergot contaminated rye feed sample. This aptamer-based extraction tool shows the applicability of aptamers for the specific extraction of toxins or natural compounds from turbid matrices in a one-step procedure.},
  author       = {Rouah-Martin, Elsa and Maho, Walid and Mehta, Jaytry and De Saeger, Sarah and Covaci, Adrian and Van Dorst, Bieke and Blust, Ronny and Robbens, Johan},
  issn         = {2156-8456},
  journal      = {ADVANCES IN BIOSCIENCE AND BIOTECHNOLOGY},
  keywords     = {Ergokryptine,Ergocornine,Ergosine,Single-Stranded Nucleic Acid Aptamer,Aptamer-Functionalized Silica Gel},
  language     = {eng},
  number       = {8},
  pages        = {692--698},
  title        = {Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed},
  url          = {http://dx.doi.org/10.4236/abb.2014.58082},
  volume       = {5},
  year         = {2014},
}

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