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Analysis of oligonucleotides using capillary zone electrophoresis and electrospray mass spectrometry

An Willems UGent, Dieter Deforce UGent and Jan Van Bocxlaer UGent (2008) Capillary electrophoresis. In Methods in Molecular Biology 384. p.401-414
abstract
This chapter illustrates the usefulness of capillary zone electrophoresis (CZE) coupled to high-resolution electrospray ionization quadrupole time-of-flight mass spectrometry for the single-step desalting, and separation, as well as characterization of oligonucleotides in the framework of quality control after synthesis. Separation is performed using a 25 mM ammonium carbonate buffer supplemented with 0.2 mM trans-1,2-diaminocyclohexane-N, N, N′, N′ id (CDTA) (pH 9.7). During the electrophoretic process, sodium and potassium ions are removed from the polyanionic backbone of the oligonucleotides by exchange of these ions with ammonium ions or by chelation on CDTA, thus eliminating a sample preparation step. A sample stacking procedure used to concentrate the samples on the CZE capillary is described. After analysis, the obtained spectrum is deconvoluted to the zero charge spectrum to yield the molecular mass of the oligonucleotide. A misincorporation of one nucleotide can be detected by a difference in mass.
Please use this url to cite or link to this publication:
author
organization
year
type
bookChapter
publication status
published
subject
book title
Capillary electrophoresis
editor
Philippe Schmitt-Kopplin
series title
Methods in Molecular Biology
volume
384
pages
401 - 414
publisher
Humana Press
place of publication
New York, NY, USA
ISBN
9781597453769
9781588295392
DOI
10.1007/978-1-59745-376-9_14
language
English
UGent publication?
yes
classification
B2
id
671376
handle
http://hdl.handle.net/1854/LU-671376
date created
2009-05-29 09:45:59
date last changed
2011-10-05 13:05:41
@incollection{671376,
  abstract     = {This chapter illustrates the usefulness of capillary zone electrophoresis (CZE) coupled to high-resolution electrospray ionization quadrupole time-of-flight mass spectrometry for the single-step desalting, and separation, as well as characterization of oligonucleotides in the framework of quality control after synthesis. Separation is performed using a 25 mM ammonium carbonate buffer supplemented with 0.2 mM trans-1,2-diaminocyclohexane-N, N, N{\textquotesingle}, N{\textquotesingle} id (CDTA) (pH 9.7). During the electrophoretic process, sodium and potassium ions are removed from the polyanionic backbone of the oligonucleotides by exchange of these ions with ammonium ions or by chelation on CDTA, thus eliminating a sample preparation step. A sample stacking procedure used to concentrate the samples on the CZE capillary is described. After analysis, the obtained spectrum is deconvoluted to the zero charge spectrum to yield the molecular mass of the oligonucleotide. A misincorporation of one nucleotide can be detected by a difference in mass.},
  author       = {Willems, An and Deforce, Dieter and Van Bocxlaer, Jan},
  booktitle    = {Capillary electrophoresis},
  editor       = {Schmitt-Kopplin, Philippe},
  isbn         = {9781597453769},
  language     = {eng},
  pages        = {401--414},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Analysis of oligonucleotides using capillary zone electrophoresis and electrospray mass spectrometry},
  url          = {http://dx.doi.org/10.1007/978-1-59745-376-9\_14},
  volume       = {384},
  year         = {2008},
}

Chicago
Willems, An, Dieter Deforce, and Jan Van Bocxlaer. 2008. “Analysis of Oligonucleotides Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry.” In Capillary Electrophoresis, ed. Philippe Schmitt-Kopplin, 384:401–414. New York, NY, USA: Humana Press.
APA
Willems, An, Deforce, D., & Van Bocxlaer, J. (2008). Analysis of oligonucleotides using capillary zone electrophoresis and electrospray mass spectrometry. In P. Schmitt-Kopplin (Ed.), Capillary electrophoresis (Vol. 384, pp. 401–414). New York, NY, USA: Humana Press.
Vancouver
1.
Willems A, Deforce D, Van Bocxlaer J. Analysis of oligonucleotides using capillary zone electrophoresis and electrospray mass spectrometry. In: Schmitt-Kopplin P, editor. Capillary electrophoresis. New York, NY, USA: Humana Press; 2008. p. 401–14.
MLA
Willems, An, Dieter Deforce, and Jan Van Bocxlaer. “Analysis of Oligonucleotides Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry.” Capillary Electrophoresis. Ed. Philippe Schmitt-Kopplin. Vol. 384. New York, NY, USA: Humana Press, 2008. 401–414. Print.